(Circular) (Six-base) MAPSORT of: pFN21AE4438.seq Check: 9057 from: 1 to: 6070 pFN21AE4438 6070 bp With 182 enzymes: SgfI * March 28, 2012 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 6070 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2013 2294 4133 5517 5785 1632 Size: 381 281 1839 1384 268 1917 Fragments arranged by size: 1917 1839 1384 381 281 268 AfeI AGC'GCT Cuts at: 3119 3119 Size: 6070 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 4969 5946 1203 Size: 3766 977 1327 Fragments arranged by size: 3766 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 2686 4799 2686 Size: 2113 3957 AleI CACnn'nnGTG Cuts at: 1166 2030 1166 Size: 864 5206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3032 3064 3032 Size: 32 6038 AlwNI CAG_nnn'CTG Cuts at: 3813 5385 3813 Size: 1572 4498 ApaLI G'TGCA_C Cuts at: 2444 2914 4127 5283 2444 Size: 470 1213 1156 3231 Fragments arranged by size: 3231 1213 1156 470 ApoI r'AATT_y Cuts at: 1493 2303 2660 3011 3379 3605 3757 5866 1493 Size: 810 357 351 368 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 368 357 351 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3395 1870 Size: 73 121 1331 4545 Fragments arranged by size: 4545 1331 121 73 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2719 3098 3391 4846 618 Size: 325 217 627 932 379 293 1455 1842 Fragments arranged by size: 1842 1455 932 627 379 325 293 217 BanII G_rGCy'C Cuts at: 1391 3389 1391 Size: 1998 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2416 2450 4261 4295 1903 Size: 34 479 34 1811 34 3678 Fragments arranged by size: 3678 1811 479 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 2448 2724 2918 3189 4131 5287 1790 Size: 658 276 194 271 942 1156 2573 Fragments arranged by size: 2573 1156 942 658 276 271 194 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2692 3801 4759 Size: 416 53 83 399 24 1275 1109 958 Cuts at: 4759 442 Size: 1753 Fragments arranged by size: 1753 1275 1109 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3044 3380 4730 1239 Size: 1805 336 1350 2579 Fragments arranged by size: 2579 1805 1350 336 Bpu10I CC'TnA_GC Cuts at: 2519 2519 Size: 6070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4195 5060 5358 4195 Size: 865 298 4907 Fragments arranged by size: 4907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4733 882 Size: 617 3234 2219 Fragments arranged by size: 3234 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2731 3861 493 Size: 711 1188 339 1130 2702 Fragments arranged by size: 2702 1188 1130 711 339 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3877 4259 4894 275 Size: 53 83 186 936 2344 382 635 1451 Fragments arranged by size: 2344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2834 2864 1178 Size: 30 165 30 1431 30 4384 Fragments arranged by size: 4384 1431 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2192 2780 3100 3326 1186 Size: 826 180 588 320 226 3930 Fragments arranged by size: 3930 826 588 320 226 180 BseYI C'CCAG_C Cuts at: 2035 2403 2647 2712 2723 3114 5273 5769 2035 Size: 368 244 65 11 391 2159 496 2336 Fragments arranged by size: 2336 2159 496 391 368 244 65 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2683 2726 1163 Size: 573 947 43 4507 Fragments arranged by size: 4507 947 573 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2448 2532 2918 4131 4216 5287 2448 Size: 84 386 1213 85 1071 3231 Fragments arranged by size: 3231 1213 1071 386 85 84 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2448 2532 2724 2918 3189 3389 4131 Size: 399 658 84 192 194 271 200 742 Cuts at: 4131 4216 5287 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 399 271 200 194 192 85 84 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 3072 3425 844 Size: 1467 761 353 3489 Fragments arranged by size: 3489 1467 761 353 BsrBI CCG'CTC Cuts at: 1394 1823 2895 3169 3364 3957 1394 Size: 429 1072 274 195 593 3507 Fragments arranged by size: 3507 1072 593 429 274 195 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2116 4130 5142 2116 Size: 2014 1012 3044 Fragments arranged by size: 3044 2014 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2181 2622 3400 3767 4152 4169 5621 5707 Size: 209 441 778 367 385 17 1452 86 Cuts at: 5707 5719 6065 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 778 441 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2512 513 Size: 552 1447 4071 Fragments arranged by size: 4071 1447 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2538 487 Size: 683 56 1312 4019 Fragments arranged by size: 4019 1312 683 56 BtsI GCAGTG_nn' Cuts at: 3586 4380 4400 3586 Size: 794 20 5256 Fragments arranged by size: 5256 794 20 ClaI AT'CG_AT Cuts at: 1554 2205 1554 Size: 651 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2610 3517 4406 Size: 54 1425 50 275 18 780 907 889 Cuts at: 4406 6035 8 Size: 1629 43 Fragments arranged by size: 1629 1425 907 889 780 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4000 1353 Size: 2647 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 2311 4657 5031 5177 387 Size: 1064 138 549 173 2346 374 146 1280 Fragments arranged by size: 2346 1280 1064 549 374 173 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2294 3044 3380 4133 4730 5517 Size: 393 381 281 750 336 753 597 787 Cuts at: 5517 5785 1239 Size: 268 1524 Fragments arranged by size: 1524 787 753 750 597 393 381 336 281 268 EcoNI CCTnn'n_nnAGG Cuts at: 3140 3140 Size: 6070 EcoO109I rG'GnC_Cy Cuts at: 2688 2688 Size: 6070 EcoRI G'AATT_C Cuts at: 2660 3011 3379 3757 2660 Size: 351 368 378 4973 Fragments arranged by size: 4973 378 368 351 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 3121 5217 1678 Size: 1443 2096 2531 Fragments arranged by size: 2531 2096 1443 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 4711 4743 4785 Size: 32 135 32 1100 32 2007 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 2007 1100 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 5184 5368 1305 Size: 984 2895 184 2007 Fragments arranged by size: 2895 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 10 Size: 54 1425 343 780 3468 Fragments arranged by size: 3468 1425 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2390 2705 4028 4387 4546 518 Size: 648 864 360 315 1323 359 159 2042 Fragments arranged by size: 2042 1323 864 648 360 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2241 2407 2716 3486 4163 5311 5556 1606 Size: 635 166 309 770 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 635 309 245 166 NcoI C'CATG_G Cuts at: 513 1065 2512 513 Size: 552 1447 4071 Fragments arranged by size: 4071 1447 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2905 3428 4973 1132 Size: 97 1676 523 1545 2229 Fragments arranged by size: 2229 1676 1545 523 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 2052 1108 Size: 944 5126 PfoI T'CCnGG_A Cuts at: 2655 2655 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PmlI CAC'GTG Cuts at: 2392 2731 2392 Size: 339 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2688 2688 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 3074 1613 Size: 1461 4609 PsiI TTA'TAA Cuts at: 2478 3640 2478 Size: 1162 4908 PstI C_TGCA'G Cuts at: 830 3422 830 Size: 2592 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2241 2407 2716 2241 Size: 166 309 5595 Fragments arranged by size: 5595 309 166 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SanDI GG'GwC_CC Cuts at: 2688 2688 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 2875 3418 4553 5234 5425 826 Size: 219 1830 543 1135 681 191 1471 Fragments arranged by size: 1830 1471 1135 681 543 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2066 3397 1872 Size: 194 1331 4545 Fragments arranged by size: 4545 1331 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 4174 5075 5337 820 Size: 197 926 2231 901 262 1553 Fragments arranged by size: 2231 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 2739 3036 1517 Size: 1222 297 4551 Fragments arranged by size: 4551 1222 297 StyI C'CwwG_G Cuts at: 513 1065 2512 3509 513 Size: 552 1447 997 3074 Fragments arranged by size: 3074 1447 997 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2088 3229 4097 4114 4267 4452 4897 Size: 1255 508 1141 868 17 153 185 445 Cuts at: 4897 325 Size: 1498 Fragments arranged by size: 1498 1255 1141 868 508 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4316 6028 96 Size: 274 80 33 51 1443 2339 1712 138 Fragments arranged by size: 2339 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 2980 3406 2980 Size: 426 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2064 3395 1870 Size: 194 1331 4545 Fragments arranged by size: 4545 1331 194 XmnI GAAnn'nnTTC Cuts at: 2597 2870 4199 2597 Size: 273 1329 4468 Fragments arranged by size: 4468 1329 273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI