(Circular) (Six-base) MAPSORT of: pFN21AE1044.seq Check: 5196 from: 1 to: 6451 pFN21AE1044 6451 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4261 278 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3794 1937 Size: 1857 4594 Acc65I G'GTAC_C Cuts at: 3772 3772 Size: 6451 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2408 2421 3588 4514 5898 6166 1632 Size: 698 78 13 1167 926 1384 268 1917 Fragments arranged by size: 1917 1384 1167 926 698 268 78 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 5350 6327 1203 Size: 4147 977 1327 Fragments arranged by size: 4147 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3054 1184 Size: 1870 4581 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3134 3166 3134 Size: 32 6419 AlwNI CAG_nnn'CTG Cuts at: 3188 4194 5766 3188 Size: 1006 1572 3873 Fragments arranged by size: 3873 1572 1006 ApaI G_GGCC'C Cuts at: 3339 3339 Size: 6451 ApaLI G'TGCA_C Cuts at: 4508 5664 4508 Size: 1156 5295 ApoI r'AATT_y Cuts at: 1493 2012 2061 2885 3116 3646 3760 3986 4138 Size: 519 49 824 231 530 114 226 152 Cuts at: 4138 6247 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 824 530 519 231 226 152 114 49 AseI AT'TA_AT Cuts at: 160 1032 5005 160 Size: 872 3973 1606 Fragments arranged by size: 3973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 3651 3776 1870 Size: 73 1708 125 4545 Fragments arranged by size: 4545 1708 125 73 BamHI G'GATC_C Cuts at: 3781 4148 3781 Size: 367 6084 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 3429 3772 5227 618 Size: 325 217 627 546 1096 343 1455 1842 Fragments arranged by size: 1842 1455 1096 627 546 343 325 217 BanII G_rGCy'C Cuts at: 1391 2901 3339 3770 1391 Size: 1510 438 431 4072 Fragments arranged by size: 4072 1510 438 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2396 3285 928 Size: 971 497 889 4094 Fragments arranged by size: 4094 971 889 497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4642 4676 1903 Size: 34 2705 34 3678 Fragments arranged by size: 3678 2705 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4343 5553 4343 Size: 1210 5241 BclI T'GATC_A Cuts at: 1548 1548 Size: 6451 BfrBI ATG'CAT Cuts at: 2459 2459 Size: 6451 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2554 3265 5062 Size: 107 122 71 631 605 882 711 1797 Cuts at: 5062 136 Size: 1525 Fragments arranged by size: 1797 1525 882 711 631 605 122 107 71 BglII A'GATC_T Cuts at: 2383 6446 2383 Size: 4063 2388 BlpI GC'TnA_GC Cuts at: 3461 3868 3461 Size: 407 6044 Bme1580I G_kGCm'C Cuts at: 1790 2255 2338 3339 3534 3585 4512 5668 1790 Size: 465 83 1001 195 51 927 1156 2573 Fragments arranged by size: 2573 1156 1001 927 465 195 83 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 5140 442 Size: 416 53 83 399 24 2765 958 1753 Fragments arranged by size: 2765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2752 2812 5111 1239 Size: 1513 60 2299 2579 Fragments arranged by size: 2579 2299 1513 60 Bpu10I CC'TnA_GC Cuts at: 3071 3071 Size: 6451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3222 4576 5441 5739 3222 Size: 1354 865 298 3934 Fragments arranged by size: 3934 1354 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2841 3236 3279 5114 882 Size: 617 1342 395 43 1835 2219 Fragments arranged by size: 2219 1835 1342 617 395 43 BsaAI yAC'GTr Cuts at: 493 1204 2951 4242 493 Size: 711 1747 1291 2702 Fragments arranged by size: 2702 1747 1291 711 BsaBI GATnn'nnATC Cuts at: 3409 6445 3409 Size: 3036 3415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4258 4640 5275 275 Size: 53 83 186 936 2725 382 635 1451 Fragments arranged by size: 2725 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4884 5282 5556 5703 6114 4884 Size: 398 274 147 411 5221 Fragments arranged by size: 5221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 3134 3164 3622 Size: 30 165 30 831 30 870 30 458 Cuts at: 3622 3652 1178 Size: 30 3977 Fragments arranged by size: 3977 870 831 458 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2498 2909 3107 3155 3543 3758 3761 Size: 1069 243 411 198 48 388 215 3 Cuts at: 3761 1186 Size: 3876 Fragments arranged by size: 3876 1069 411 388 243 215 198 48 3 BseYI C'CCAG_C Cuts at: 3181 3231 3356 3437 3722 5654 6150 3181 Size: 50 125 81 285 1932 496 3482 Fragments arranged by size: 3482 1932 496 285 125 81 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2877 1163 Size: 573 1141 4737 Fragments arranged by size: 4737 1141 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3901 4662 5690 1540 Size: 275 186 1900 761 1028 2301 Fragments arranged by size: 2301 1900 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2901 4512 4597 5668 2901 Size: 1611 85 1071 3684 Fragments arranged by size: 3684 1611 1071 85 BsmI GAATG_Cn' Cuts at: 3962 4055 3962 Size: 93 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2338 2901 3339 3534 3585 3770 Size: 399 465 83 563 438 195 51 185 Cuts at: 3770 4512 4597 5668 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 465 438 399 195 185 85 83 51 BspEI T'CCGG_A Cuts at: 6114 6114 Size: 6451 BspHI T'CATG_A Cuts at: 4340 6070 4340 Size: 1730 4721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2201 3806 844 Size: 1357 1605 3489 Fragments arranged by size: 3489 1605 1357 BsrBI CCG'CTC Cuts at: 1394 1823 4338 1394 Size: 429 2515 3507 Fragments arranged by size: 3507 2515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2374 4946 5120 65 Size: 1183 1126 2572 174 1396 Fragments arranged by size: 2572 1396 1183 1126 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5095 5282 1700 Size: 110 3285 187 2869 Fragments arranged by size: 3285 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 4511 5523 4511 Size: 1012 5439 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6451 BstBI TT'CG_AA Cuts at: 5255 5255 Size: 6451 BstEII G'GTnAC_C Cuts at: 1184 2188 3299 3655 1184 Size: 1004 1111 356 3980 Fragments arranged by size: 3980 1111 1004 356 BstXI CCAn_nnnn'nTGG Cuts at: 2822 2995 2822 Size: 173 6278 BstYI r'GATC_y Cuts at: 1972 2091 2383 3781 4148 4533 4550 6002 6088 Size: 119 292 1398 367 385 17 1452 86 Cuts at: 6088 6100 6446 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1398 385 367 346 292 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4960 Fragments arranged by size: 4960 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5712 Fragments arranged by size: 5712 683 56 BtsI GCAGTG_nn' Cuts at: 3186 3967 4761 4781 3186 Size: 781 794 20 4856 Fragments arranged by size: 4856 794 781 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2683 2718 529 Size: 35 2119 35 4262 Fragments arranged by size: 4262 2119 35 35 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DraIII CAC_nnn'GTG Cuts at: 3088 3088 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5458 809 Size: 507 4142 1802 Fragments arranged by size: 4142 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3898 4787 6416 Size: 54 1425 50 275 18 2068 889 1629 Cuts at: 6416 8 Size: 43 Fragments arranged by size: 2068 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3898 1537 Size: 275 2086 4090 Fragments arranged by size: 4090 2086 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2703 2986 3132 3729 3735 4381 1353 Size: 860 490 283 146 597 6 646 3423 Fragments arranged by size: 3423 860 646 597 490 283 146 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3508 5038 5412 5558 387 Size: 1064 138 1919 1530 374 146 1280 Fragments arranged by size: 1919 1530 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2899 2899 Size: 6451 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2408 2421 2752 2812 3588 4514 Size: 393 698 78 13 331 60 776 926 Cuts at: 4514 5111 5898 6166 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 926 787 776 698 597 393 331 268 78 60 13 EcoO109I rG'GnC_Cy Cuts at: 2556 3416 2556 Size: 860 5591 EcoRI G'AATT_C Cuts at: 3116 3646 3760 4138 3116 Size: 530 114 378 5429 Fragments arranged by size: 5429 530 378 114 FspI TGC'GCA Cuts at: 4957 4957 Size: 6451 HaeII r_GCGC'y Cuts at: 1678 5598 1678 Size: 3920 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 2911 2943 3535 Size: 32 135 32 511 32 796 32 592 Cuts at: 3535 3567 5092 5124 5166 5198 1373 Size: 32 1525 32 42 32 2626 Fragments arranged by size: 2626 1525 796 592 511 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3795 4041 669 Size: 988 281 1857 246 3079 Fragments arranged by size: 3079 1857 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6451 HpaI GTT'AAC Cuts at: 4041 4041 Size: 6451 KpnI G_GTAC'C Cuts at: 3776 3776 Size: 6451 MfeI C'AATT_G Cuts at: 4050 4050 Size: 6451 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 5565 5749 1305 Size: 888 3372 184 2007 Fragments arranged by size: 3372 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4629 Fragments arranged by size: 4629 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 4409 4768 4927 518 Size: 648 948 2295 359 159 2042 Fragments arranged by size: 2295 2042 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2861 3133 3668 3867 4544 5692 5937 1606 Size: 1255 272 535 199 677 1148 245 2120 Fragments arranged by size: 2120 1255 1148 677 535 272 245 199 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4960 Fragments arranged by size: 4960 939 552 NdeI CA'TA_TG Cuts at: 387 2461 387 Size: 2074 4377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6451 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NsiI A_TGCA'T Cuts at: 2461 2461 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 3809 5354 1132 Size: 97 2580 1545 2229 Fragments arranged by size: 2580 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3527 3596 1348 Size: 2179 69 4203 Fragments arranged by size: 4203 2179 69 PciI A'CATG_T Cuts at: 5350 5350 Size: 6451 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6451 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PmlI CAC'GTG Cuts at: 2951 2951 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3134 3166 4520 4552 6033 6065 3134 Size: 32 1354 32 1481 32 3520 Fragments arranged by size: 3520 1481 1354 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6451 PsiI TTA'TAA Cuts at: 4021 4021 Size: 6451 PspOMI G'GGCC_C Cuts at: 3335 3335 Size: 6451 PstI C_TGCA'G Cuts at: 830 2176 3667 3803 830 Size: 1346 1491 136 3478 Fragments arranged by size: 3478 1491 1346 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 PvuII CAG'CTG Cuts at: 2861 3133 3668 2861 Size: 272 535 5644 Fragments arranged by size: 5644 535 272 SacI G_AGCT'C Cuts at: 2901 2901 Size: 6451 SalI G'TCGA_C Cuts at: 1936 3793 1936 Size: 1857 4594 SapI GCTCTTCn'nnn_ Cuts at: 2986 2986 Size: 6451 SbfI CC_TGCA'GG Cuts at: 3803 3803 Size: 6451 ScaI AGT'ACT Cuts at: 4699 6411 4699 Size: 1712 4739 SfcI C'TryA_G Cuts at: 826 1045 2172 3663 3799 4934 5615 5806 826 Size: 219 1127 1491 136 1135 681 191 1471 Fragments arranged by size: 1491 1471 1135 1127 681 219 191 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 3653 3778 1872 Size: 1781 125 4545 Fragments arranged by size: 4545 1781 125 SmlI C'TyrA_G Cuts at: 820 1017 1943 3237 4555 5456 5718 820 Size: 197 926 1294 1318 901 262 1553 Fragments arranged by size: 1553 1318 1294 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4242 493 Size: 3749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6451 SphI G_CATG'C Cuts at: 1132 3809 1132 Size: 2677 3774 SspI AAT'ATT Cuts at: 5 52 4375 5 Size: 47 4323 2081 Fragments arranged by size: 4323 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6451 StyI C'CwwG_G Cuts at: 513 1065 2004 3890 513 Size: 552 939 1886 3074 Fragments arranged by size: 3074 1886 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 3417 4478 4495 4648 4833 5278 Size: 1255 971 866 1061 17 153 185 445 Cuts at: 5278 325 Size: 1498 Fragments arranged by size: 1498 1255 1061 971 866 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4697 6409 96 Size: 274 80 33 51 1443 2720 1712 138 Fragments arranged by size: 2720 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 3622 3654 6411 6443 2234 Size: 32 1356 32 2757 32 2242 Fragments arranged by size: 2757 2242 1356 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6451 XbaI T'CTAG_A Cuts at: 2017 3787 2017 Size: 1770 4681 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 2152 Size: 6451 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6451 XmaI C'CCGG_G Cuts at: 1870 3651 3776 1870 Size: 1781 125 4545 Fragments arranged by size: 4545 1781 125 XmnI GAAnn'nnTTC Cuts at: 2139 4580 2139 Size: 2441 4010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4259 276 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI