################################## # NO CUT ENZYMES ( 213 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 226 CCGC CCGC(-3/-1) AlwNI 44 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 67 GGACC G^G[AT]CC BbsI 272 GTCTTC GAAGAC(2/6) BbvI 258 GCAGC GCAGC(8/12) BfaI 131 CTAG C^TAG BpuAI 272 GTCTTC GAAGAC(2/6) BsgI 256 GTGCAG GTGCAG(16/14) BsmAI 328 GAGAC GTCTC(1/5) BsmI 3 GAATGC GAATGC(1/-1) BspHI 81 TCATGA T^CATGA BspMI 261 GCAGGT ACCTGC(4/8) BstYI 19 GGATCT [GA]^GATC[CT] Cfr13I 67 GGACC G^G[ACGT]CC EcoRI 244 GAATTC G^AATTC Fnu4HI 258 GCAGC GC^[ACGT]GC HgaI 270 GCGTC GACGC(5/10) ItaI 258 GCAGC GC^[ACGT]GC MaeI 131 CTAG C^TAG MaeIII 46 GTTAC ^GT[ACGT]AC MflI 19 GGATCT [GA]^GATC[CT] MseI 353 TTAA T^TAA MwoI 261 GCAGGTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC RcaI 81 TCATGA T^CATGA Sau96I 67 GGACC G^G[ACGT]CC Tru9I 353 TTAA T^TAA TseI 258 GCAGC G^C[AT]GC XhoII 19 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### AlwI 358 GATCC GGATC(4/5) 19 GGATC BsrI 303 ACTGG ACTGG(1/-1) 43 CCAGT BstF5I 136 CATCC GGATG(2/0) 306 GGATG DdeI 23 CTGAG C^T[ACGT]AG 199 CTGAG FokI 136 CATCC GGATG(9/13) 306 GGATG NlaIII 82 CATG CATG^ 378 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AcsI AluI ApoI DpnII MboI MboII MnlI NdeII Sau3AI Tsp509I TspRI