################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NciI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 144 CGCG CG^CG AcyI 12 GGCGCC G[GA]^CG[CT]C AfaI 193 GTAC GT^AC Alw44I 169 GTGCAC G^TGCAC ApaLI 169 GTGCAC G^TGCAC AspHI 169 GTGCAC G[AT]GC[AT]^C AvaII 71 GGTCC G^G[AT]CC BanI 12 GGCGCC G^G[CT][GA]CC BbeI 12 GGCGCC GGCGC^C BbvI 27 GCAGC GCAGC(8/12) BmyI 169 GTGCAC G[AGT]GC[ACT]^C BsaHI 12 GGCGCC G[GA]^CG[CT]C BsaI 43 GAGACC GGTCTC(1/5) BsiHKAI 169 GTGCAC G[AT]GC[AT]^C BsmAI 43 GAGAC GTCTC(1/5) Bsp1286I 169 GTGCAC G[AGT]GC[ACT]^C BspHI 222 TCATGA T^CATGA BspMI 197 ACCTGC ACCTGC(4/8) BsrFI 30 GCCGGC [GA]^CCGG[CT] BstUI 144 CGCG CG^CG Cac8I 30 GCCGGC GC[ACGT]^[ACGT]GC CfoI 13 GCGC GCG^C Cfr10I 30 GCCGGC [GA]^CCGG[CT] Cfr13I 71 GGTCC G^G[ACGT]CC DraII 70 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 32 CGGCCA [CT]^GGCC[GA] EarI 82 GAAGAG CTCTTC(1/4) EcoO109I 70 AGGTCCC [GA]G^G[ACGT]CC[CT] Fnu4HI 27 GCAGC GC^[ACGT]GC HaeII 12 GGCGCC [GA]GCGC^[CT] HapII 31 CCGG C^CGG HhaI 13 GCGC GCG^C Hin1I 12 GGCGCC G[GA]^CG[CT]C HinP1I 13 GCGC G^CGC HpaII 31 CCGG C^CGG HphI 66 GGTGA GGTGA(8/7) ItaI 27 GCAGC GC^[ACGT]GC KasI 12 GGCGCC G^GCGCC Ksp632I 82 GAAGAG CTCTTC(1/4) MaeII 173 ACGT A^CGT MboII 82 GAAGA GAAGA(8/7) MseI 113 TTAA T^TAA MslI 162 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 31 CCGG C^CGG MvnI 144 CGCG CG^CG NaeI 30 GCCGGC GCC^GGC NarI 12 GGCGCC GG^CGCC NgoMIV 30 GCCGGC G^CCGGC PpuMI 70 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 222 TCATGA T^CATGA RsaI 193 GTAC GT^AC Sau96I 71 GGTCC G^G[ACGT]CC SexAI 62 ACCAGGT A^CC[AT]GGT SfoI 12 GGCGCC GGC^GCC Tru9I 113 TTAA T^TAA TseI 27 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 77 AGCT AG^CT 281 AGCT BsmFI 148 GGGAC GGGAC(10/14) 72 GTCCC BssKI 63 CCAGG ^CC[ACGT]GG 135 CCAGG BstNI 63 CCAGG CC^[AT]GG 135 CCAGG EcoRII 63 CCAGG ^CC[AT]GG 135 CCAGG FauI 8 GCGGG CCCGC(4/6) 145 GCGGG HaeIII 33 GGCC GG^CC 117 GGCC MvaI 63 CCAGG CC^[AT]GG 135 CCAGG MwoI 13 GCGCCCTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 273 GCATGATGAGC NlaIII 223 CATG CATG^ 274 CATG NlaIV 12 GGCGCC GG[ACGT]^[ACGT]CC 71 GGTCCC ScrFI 63 CCAGG CC^[ACGT]GG 135 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### AciI MnlI