Restriction map for
pFN21AE5125
(Circular) (Six-base) MAPSORT of: pFN21AE5125.seq Check: 7062 from: 1 to: 4999
pFN21AE5125 4999 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 2211
Size: 4999
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2809 278
Size: 53 83 186 936 1273 2468
Fragments arranged by size:
2468 1273 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2342 1937
Size: 405 4594
Acc65I G'GTAC_C
Cuts at: 2320 2320
Size: 4999
AclI AA'CG_TT
Cuts at: 1563 3126 3499 1563
Size: 1563 373 3063
Fragments arranged by size:
3063 1563 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 4446 4714 1632
Size: 1430 1384 268 1917
Fragments arranged by size:
1917 1430 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4802
AflIII A'CryG_T
Cuts at: 1203 3898 4875 1203
Size: 2695 977 1327
Fragments arranged by size:
2695 1327 977
AgeI A'CCGG_T
Cuts at: 3830 3830
Size: 4999
AhdI GACnn_n'nnGTC
Cuts at: 3728 3728
Size: 4999
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4999
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4999
AlwNI CAG_nnn'CTG
Cuts at: 2742 4314 2742
Size: 1572 3427
ApaLI G'TGCA_C
Cuts at: 2174 3056 4212 2174
Size: 882 1156 2961
Fragments arranged by size:
2961 1156 882
ApoI r'AATT_y
Cuts at: 1493 2308 2534 2686 4795 1493
Size: 815 226 152 2109 1697
Fragments arranged by size:
2109 1697 815 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3553 160
Size: 872 2521 1606
Fragments arranged by size:
2521 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4999
AvaI C'yCGr_G
Cuts at: 1870 1943 2324 1870
Size: 73 381 4545
Fragments arranged by size:
4545 381 73
BamHI G'GATC_C
Cuts at: 2329 2696 2329
Size: 367 4632
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2320 3775 618
Size: 325 217 627 230 303 1455 1842
Fragments arranged by size:
1842 1455 627 325 303 230 217
BanII G_rGCy'C
Cuts at: 1391 2318 1391
Size: 927 4072
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 4999
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3190 3224 1903
Size: 34 1253 34 3678
Fragments arranged by size:
3678 1253 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2891 4101 2891
Size: 1210 3789
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4999
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3610 136
Size: 107 122 71 631 605 1938 1525
Fragments arranged by size:
1938 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4994 4994
Size: 4999
BlpI GC'TnA_GC
Cuts at: 2416 2416
Size: 4999
Bme1580I G_kGCm'C
Cuts at: 1790 2178 3060 4216 1790
Size: 388 882 1156 2573
Fragments arranged by size:
2573 1156 882 388
BmgBI CAC'GTC
Cuts at: 1318 2179 1318
Size: 861 4138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2730 3688 442
Size: 416 53 83 399 24 1313 958 1753
Fragments arranged by size:
1753 1313 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3659 1239
Size: 2420 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3124 3989 4287 3124
Size: 865 298 3836
Fragments arranged by size:
3836 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 3662 882
Size: 617 543 1620 2219
Fragments arranged by size:
2219 1620 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 2790 493
Size: 711 1586 2702
Fragments arranged by size:
2702 1586 711
BsaBI GATnn'nnATC
Cuts at: 4993 4993
Size: 4999
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2806 3188 3823
Size: 53 83 186 936 485 788 382 635
Cuts at: 3823 275
Size: 1451
Fragments arranged by size:
1451 936 788 635 485 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3432 3830 4104 4251 4662 3432
Size: 398 274 147 411 3769
Fragments arranged by size:
3769 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4774
Fragments arranged by size:
4774 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4999
BseYI C'CCAG_C
Cuts at: 2052 2079 4202 4698 2052
Size: 27 2123 496 2353
Fragments arranged by size:
2353 2123 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2449 3210 4238 1540
Size: 275 186 448 761 1028 2301
Fragments arranged by size:
2301 1028 761 448 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 3060 3145 4216 2178
Size: 882 85 1071 2961
Fragments arranged by size:
2961 1071 882 85
BsmI GAATG_Cn'
Cuts at: 2510 2603 2510
Size: 93 4906
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2318 3060 3145 4216 1391
Size: 399 388 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 140 85
BspEI T'CCGG_A
Cuts at: 4662 4662
Size: 4999
BspHI T'CATG_A
Cuts at: 2227 2888 4618 2227
Size: 661 1730 2608
Fragments arranged by size:
2608 1730 661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2354 844
Size: 1367 143 3489
Fragments arranged by size:
3489 1367 143
BsrBI CCG'CTC
Cuts at: 1394 1823 2886 1394
Size: 429 1063 3507
Fragments arranged by size:
3507 1063 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3494 3668 65
Size: 1183 2246 174 1396
Fragments arranged by size:
2246 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 3643 3830 1700
Size: 110 225 1608 187 2869
Fragments arranged by size:
2869 1608 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4999
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4999
BssSI C'ACGA_G
Cuts at: 3059 4071 3059
Size: 1012 3987
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4999
BstBI TT'CG_AA
Cuts at: 3803 3803
Size: 4999
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4999
BstYI r'GATC_y
Cuts at: 1972 2329 2696 3081 3098 4550 4636 4648 4994
Size: 357 367 385 17 1452 86 12 346
Cuts at: 4994 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 357 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 487
Size: 683 56 818 3442
Fragments arranged by size:
3442 818 683 56
BtsI GCAGTG_nn'
Cuts at: 2515 3309 3329 2515
Size: 794 20 4185
Fragments arranged by size:
4185 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4964
DraI TTT'AAA
Cuts at: 2303 2303
Size: 4999
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4006 809
Size: 507 2690 1802
Fragments arranged by size:
2690 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2446 3335
Size: 54 1425 50 275 18 207 409 889
Cuts at: 3335 4964 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 409 275 207 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2446 1537
Size: 275 634 4090
Fragments arranged by size:
4090 634 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2929 1353
Size: 729 847 3423
Fragments arranged by size:
3423 847 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2014 3586 3960 4106 387
Size: 1064 138 425 1572 374 146 1280
Fragments arranged by size:
1572 1280 1064 425 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3062 3659 4446 4714 1239
Size: 393 1430 597 787 268 1524
Fragments arranged by size:
1524 1430 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2076 2076
Size: 4999
EcoRI G'AATT_C
Cuts at: 2308 2686 2308
Size: 378 4621
FspI TGC'GCA
Cuts at: 3505 3505
Size: 4999
HaeII r_GCGC'y
Cuts at: 1678 2021 4146 1678
Size: 343 2125 2531
Fragments arranged by size:
2531 2125 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3640 3672 3714 3746 1373
Size: 32 135 32 2068 32 42 32 2626
Fragments arranged by size:
2626 2068 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2343 2589 669
Size: 988 281 405 246 3079
Fragments arranged by size:
3079 988 405 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4999
HpaI GTT'AAC
Cuts at: 2589 2589
Size: 4999
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 4999
KpnI G_GTAC'C
Cuts at: 2324 2324
Size: 4999
MfeI C'AATT_G
Cuts at: 2598 2598
Size: 4999
MluI A'CGCG_T
Cuts at: 4875 4875
Size: 4999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4113 4297 1305
Size: 2808 184 2007
Fragments arranged by size:
2808 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3177
Fragments arranged by size:
3177 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2957 3316 3475 518
Size: 648 1005 786 359 159 2042
Fragments arranged by size:
2042 1005 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2415 3092 4240 4485 1606
Size: 809 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 245
NaeI GCC'GGC
Cuts at: 2037 2037
Size: 4999
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 4999
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4999
NgoMIV G'CCGG_C
Cuts at: 2035 2035
Size: 4999
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4999
NotI GC'GGCC_GC
Cuts at: 2446 2446
Size: 4999
NspI r_CATG'y
Cuts at: 1132 1229 2357 3902 1132
Size: 97 1128 1545 2229
Fragments arranged by size:
2229 1545 1128 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4999
PciI A'CATG_T
Cuts at: 3898 3898
Size: 4999
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4999
PmeI GTTT'AAAC
Cuts at: 2303 2303
Size: 4999
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 4581 4613 3068
Size: 32 1481 32 3454
Fragments arranged by size:
3454 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2076
Size: 4999
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4999
PsiI TTA'TAA
Cuts at: 2569 2569
Size: 4999
PstI C_TGCA'G
Cuts at: 830 2351 830
Size: 1521 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4999
SalI G'TCGA_C
Cuts at: 1936 2341 1936
Size: 405 4594
SbfI CC_TGCA'GG
Cuts at: 2351 2351
Size: 4999
ScaI AGT'ACT
Cuts at: 3247 4959 3247
Size: 1712 3287
SexAI A'CCwGG_T
Cuts at: 2067 2067
Size: 4999
SfcI C'TryA_G
Cuts at: 826 1045 2347 3482 4163 4354 826
Size: 219 1302 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 4999
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4999
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4999
SmaI CCC'GGG
Cuts at: 1872 2326 1872
Size: 454 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3103 4004 4266 820
Size: 197 926 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2790 493
Size: 2297 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4999
SphI G_CATG'C
Cuts at: 1132 2357 1132
Size: 1225 3774
SspI AAT'ATT
Cuts at: 5 52 2923 5
Size: 47 2871 2081
Fragments arranged by size:
2871 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4999
StyI C'CwwG_G
Cuts at: 513 1065 2438 513
Size: 552 1373 3074
Fragments arranged by size:
3074 1373 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3026 3043 3196 3381 3826 325
Size: 1255 1446 17 153 185 445 1498
Fragments arranged by size:
1498 1446 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3245 4957 96
Size: 274 80 33 51 1443 1268 1712 138
Fragments arranged by size:
1712 1443 1268 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4959 4991 4959
Size: 32 4967
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4999
XbaI T'CTAG_A
Cuts at: 2335 2335
Size: 4999
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4999
XmaI C'CCGG_G
Cuts at: 1870 2324 1870
Size: 454 4545
XmnI GAAnn'nnTTC
Cuts at: 3128 3128
Size: 4999
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2807 276
Size: 53 83 186 936 1273 2468
Fragments arranged by size:
2468 1273 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI XcmI