(Linear) (Six-base) MAPSORT of: KIEE5125.seq  Check: 7323  from: 1  to: 294



KIEE5125.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     206     294 
   Size:        206      88
 
ApaLI G'TGCA_C

Cuts at:      0     169     294 
   Size:        169     125
 
BanI G'GyrC_C

Cuts at:      0      12     294 
   Size:         12     282
 
BbeI G_GCGC'C

Cuts at:      0      16     294 
   Size:         16     278
 
Bme1580I G_kGCm'C

Cuts at:      0     173     294 
   Size:        173     121
 
BmgBI CAC'GTC

Cuts at:      0     174     294 
   Size:        174     120
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37     294 
   Size:         37     257
 
BsaHI Gr'CG_yC

Cuts at:      0      13     294 
   Size:         13     281
 
BseYI C'CCAG_C

Cuts at:      0      47      74     294 
   Size:         47      27     220

  Fragments arranged by size: 

                 220      47      27
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     294 
   Size:        173     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     294 
   Size:        173     121
 
BspHI T'CATG_A

Cuts at:      0     222     294 
   Size:        222      72
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     206     294 
   Size:        206      88
 
BsrFI r'CCGG_y

Cuts at:      0      30     294 
   Size:         30     264
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      39     294 
   Size:         39     255
 
EaeI y'GGCC_r

Cuts at:      0      32     294 
   Size:         32     262
 
EarI CTCTTCn'nnn_

Cuts at:      0      77     294 
   Size:         77     217
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       9     294 
   Size:          9     285
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     294 
   Size:         71     223
 
HaeII r_GCGC'y

Cuts at:      0      16     294 
   Size:         16     278
 
KasI G'GCGC_C

Cuts at:      0      12     294 
   Size:         12     282
 
MslI CAynn'nnrTG

Cuts at:      0     166     294 
   Size:        166     128
 
NaeI GCC'GGC

Cuts at:      0      32     294 
   Size:         32     262
 
NarI GG'CG_CC

Cuts at:      0      13     294 
   Size:         13     281
 
NgoMIV G'CCGG_C

Cuts at:      0      30     294 
   Size:         30     264
 
PpuMI rG'GwC_Cy

Cuts at:      0      71     294 
   Size:         71     223
 
SexAI A'CCwGG_T

Cuts at:      0      62     294 
   Size:         62     232
 
SfoI GGC'GCC

Cuts at:      0      14     294 
   Size:         14     280
 

 Enzymes that do cut:

     AarI    ApaLI     BanI     BbeI Bme1580I    BmgBI     BsaI    BsaHI
    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI    BtgZI     EaeI
     EarI     EciI EcoO109I    HaeII     KasI     MslI     NaeI     NarI
   NgoMIV    PpuMI    SexAI     SfoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI