Restriction map for
pFN21AE1555
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeIII MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MunI NaeI
NarI NciI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 151 GTATAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AcsI 371 GAATTT [GA]^AATT[CT]
Aor51HI 313 AGCGCT AGC^GCT
ApoI 371 GAATTT [GA]^AATT[CT]
AseI 382 ATTAAT AT^TAAT
AsnI 382 ATTAAT AT^TAAT
BmyI 46 GTGCCC G[AGT]GC[ACT]^C
BpmI 261 CTGGAG CTGGAG(16/14)
BsaBI 251 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 184 GTCTC GTCTC(1/5)
BsmFI 289 GGGAC GGGAC(10/14)
Bsp1286I 46 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 305 TGTACA T^GTACA
BsrGI 305 TGTACA T^GTACA
BsrI 295 ACTGG ACTGG(1/-1)
BssKI 260 CCTGG ^CC[ACGT]GG
Bst1107I 151 GTATAC GTA^TAC
BstNI 260 CCTGG CC^[AT]GG
BstUI 60 CGCG CG^CG
BstZ17I 151 GTATAC GTA^TAC
Cac8I 18 GCCCGC GC[ACGT]^[ACGT]GC
DdeI 350 CTGAG C^T[ACGT]AG
Eco47III 313 AGCGCT AGC^GCT
EcoRII 260 CCTGG ^CC[AT]GG
EcoT14I 128 CCATGG C^C[AT][AT]GG
HaeII 313 AGCGCT [GA]GCGC^[CT]
HaeIII 3 GGCC GG^CC
HinfI 256 GAATC G^A[ACGT]TC
MaeII 44 ACGT A^CGT
MamI 251 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 383 TTAA T^TAA
MvaI 260 CCTGG CC^[AT]GG
MvnI 60 CGCG CG^CG
NcoI 128 CCATGG C^CATGG
NspI 333 ACATGC [GA]CATG^[CT]
PshBI 382 ATTAAT AT^TAAT
ScrFI 260 CCTGG CC^[ACGT]GG
SspBI 305 TGTACA T^GTACA
StyI 128 CCATGG C^C[AT][AT]GG
TfiI 256 GAATC G^A[AT]TC
Tru9I 383 TTAA T^TAA
TspRI 294 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 285 CCACGGGACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 28 AGCT AG^CT
101 AGCT
AvaI 51 CTCGGG C^[CT]CG[GA]G
97 CCCGAG
BsoBI 51 CTCGGG C^[CT]CG[GA]G
97 CCCGAG
CfoI 61 GCGC GCG^C
314 GCGC
DsaI 128 CCATGG C^C[GA][CT]GG
285 CCACGG
FauI 15 CCCGC CCCGC(4/6)
19 CCCGC
HhaI 61 GCGC GCG^C
314 GCGC
HinP1I 61 GCGC G^CGC
314 GCGC
MboII 89 TCTTC GAAGA(8/7)
401 TCTTC
MwoI 9 GCCCGACCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
406 GCCTTCATCGC
NlaIV 289 GGGACC GG[ACGT]^[ACGT]CC
363 GGACCC
SfaNI 299 GCATC GCATC(5/9)
446 GCATC
Tsp509I 372 AATT ^AATT
385 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AfaI AvaII BbvI BsaJI
BstF5I Cfr13I Fnu4HI FokI HphI
ItaI MnlI NlaIII RsaI Sau96I
TseI