################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AhdI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII BalI BanI BbeI BbrPI BcgI BfrI BglI BglII BsaI BseAI BsiEI BsiWI BsmFI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CfoI ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MluI MluNI MroI MscI MslI NarI NcoI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AatII 1804 GACGTC GACGT^C AccII 11 CGCG CG^CG AcyI 1804 GACGTC G[GA]^CG[CT]C AflIII 198 ACATGT A^C[GA][CT]GT AgeI 115 ACCGGT A^CCGGT Alw44I 1295 GTGCAC G^TGCAC AocI 1080 CCTCAGG CC^T[ACGT]AGG ApaI 1663 GGGCCC GGGCC^C ApaLI 1295 GTGCAC G^TGCAC AvaI 23 CCCGGG C^[CT]CG[GA]G AvrII 1611 CCTAGG C^CTAGG BamHI 1302 GGATCC G^GATCC BbsI 1606 GAAGAC GAAGAC(2/6) BbvCI 34 GCTGAGG CCTCAGC(-5/-2) BciVI 147 GGATAC GTATCC(6/5) BlnI 1611 CCTAGG C^CTAGG BpuAI 1606 GAAGAC GAAGAC(2/6) BsaAI 1874 CACGTA [CT]AC^GT[GA] BsaHI 1804 GACGTC G[GA]^CG[CT]C BsaWI 115 ACCGGT [AT]^CCGG[AT] BseRI 94 CTCCTC GAGGAG(10/8) BsgI 1236 GTGCAG GTGCAG(16/14) BsmBI 1489 GAGACG CGTCTC(1/5) BsoBI 23 CCCGGG C^[CT]CG[GA]G Bsp1407I 517 TGTACA T^GTACA BspHI 287 TCATGA T^CATGA BspLU11I 198 ACATGT A^CATGT BspMI 930 GCAGGT ACCTGC(4/8) BsrGI 517 TGTACA T^GTACA BssSI 106 CTCGTG CACGAG(-5/-1) BstUI 11 CGCG CG^CG BstXI 582 CCAGTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1302 GGATCC [GA]^GATC[CT] Bsu36I 1080 CCTCAGG CC^T[ACGT]AGG DraI 775 TTTAAA TTT^AAA DraII 1662 GGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 44 TGGCCG [CT]^GGCC[GA] Eco81I 1080 CCTCAGG CC^T[ACGT]AGG EcoNI 1611 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1662 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 1123 GATATC GAT^ATC Hin1I 1804 GACGTC G[GA]^CG[CT]C HincII 798 GTTGAC GT[CT]^[GA]AC HindII 798 GTTGAC GT[CT]^[GA]AC MfeI 1317 CAATTG C^AATTG MflI 1302 GGATCC [GA]^GATC[CT] MunI 1317 CAATTG C^AATTG MvnI 11 CGCG CG^CG NaeI 1544 GCCGGC GCC^GGC NdeI 1026 CATATG CA^TATG NgoMIV 1544 GCCGGC G^CCGGC NspI 198 ACATGT [GA]CATG^[CT] PinAI 115 ACCGGT A^CCGGT PstI 241 CTGCAG CTGCA^G RcaI 287 TCATGA T^CATGA SapI 1891 GAAGAGC GCTCTTC(1/4) ScaI 569 AGTACT AGT^ACT SfcI 241 CTGCAG C^T[GA][CT]AG SmaI 23 CCCGGG CCC^GGG SspBI 517 TGTACA T^GTACA StuI 915 AGGCCT AGG^CCT XbaI 936 TCTAGA T^CTAGA XcmI 1440 CCAAATTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1302 GGATCC [GA]^GATC[CT] XmaCI 23 CCCGGG C^CCGGG XmaI 23 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BclI 606 TGATCA T^GATCA 1586 TGATCA BlpI 1018 GCTGAGC GC^T[ACGT]AGC 1720 GCTCAGC BpmI 833 CTCCAG CTGGAG(16/14) 1567 CTCCAG Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC 1720 GCTCAGC BsaBI 601 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1587 GATCAACATC BsmAI 979 GAGAC GTCTC(1/5) 1489 GAGAC BsmI 339 GAATGC GAATGC(1/-1) 882 GAATGC BsrDI 550 CATTGC GCAATG(2/0) 1051 GCAATG BsrFI 115 ACCGGT [GA]^CCGG[CT] 1544 GCCGGC CelII 1018 GCTGAGC GC^T[ACGT]AGC 1720 GCTCAGC Cfr10I 115 ACCGGT [GA]^CCGG[CT] 1544 GCCGGC EcoT14I 227 CCAAGG C^C[AT][AT]GG 1611 CCTAGG EcoT22I 884 ATGCAT ATGCA^T 1087 ATGCAT FauI 12 GCGGG CCCGC(4/6) 73 GCGGG FbaI 606 TGATCA T^GATCA 1586 TGATCA HindIII 769 AAGCTT A^AGCTT 1279 AAGCTT MamI 601 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1587 GATCAACATC MspA1I 392 CAGCTG C[AC]G^C[GT]G 422 CAGCTG NsiI 884 ATGCAT ATGCA^T 1087 ATGCAT PvuII 392 CAGCTG CAG^CTG 422 CAGCTG SmlI 97 CTCAAG C^T[CT][GA]AG 1742 CTCAAG StyI 227 CCAAGG C^C[AT][AT]GG 1611 CCTAGG TaqI 131 TCGA T^CGA 977 TCGA TthHB8I 131 TCGA T^CGA 977 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BanII BbvI BcnI BfaI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI SacI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI