(Circular) (Six-base) MAPSORT of: pFN21AB6150.seq Check: 55 from: 1 to: 6625 pFN21AB6150 6625 bp With 182 enzymes: SgfI * March 28, 2012 12:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3813 4435 278 Size: 53 83 186 936 2277 622 2468 Fragments arranged by size: 2468 2277 936 622 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 2664 2880 3702 4688 6072 6340 1632 Size: 894 138 216 822 986 1384 268 1917 Fragments arranged by size: 1917 1384 986 894 822 268 216 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 2203 5524 6501 1203 Size: 1000 3321 977 1327 Fragments arranged by size: 3321 1327 1000 977 AgeI A'CCGG_T Cuts at: 2120 5456 2120 Size: 3336 3289 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AlwNI CAG_nnn'CTG Cuts at: 4368 5940 4368 Size: 1572 5053 ApaI G_GGCC'C Cuts at: 3672 3672 Size: 6625 ApaLI G'TGCA_C Cuts at: 3300 4682 5838 3300 Size: 1382 1156 4087 Fragments arranged by size: 4087 1382 1156 ApoI r'AATT_y Cuts at: 1493 2315 3419 3527 3632 3934 4160 4312 6421 Size: 822 1104 108 105 302 226 152 2109 Cuts at: 6421 1493 Size: 1697 Fragments arranged by size: 2109 1697 1104 822 302 226 152 108 105 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2028 3950 1870 Size: 73 85 1922 4545 Fragments arranged by size: 4545 1922 85 73 AvrII C'CTAG_G Cuts at: 3616 3616 Size: 6625 BamHI G'GATC_C Cuts at: 3307 3955 4322 3307 Size: 648 367 5610 Fragments arranged by size: 5610 648 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3946 5401 618 Size: 325 217 627 2159 1455 1842 Fragments arranged by size: 2159 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2100 3030 3672 3727 3944 1391 Size: 709 930 642 55 217 4072 Fragments arranged by size: 4072 930 709 642 217 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3618 928 Size: 971 1719 3935 Fragments arranged by size: 3935 1719 971 BbvCI CC'TCA_GC Cuts at: 2040 2040 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2146 4517 5727 2146 Size: 2371 1210 3044 Fragments arranged by size: 3044 2371 1210 BclI T'GATC_A Cuts at: 1548 2611 3591 1548 Size: 1063 980 4582 Fragments arranged by size: 4582 1063 980 BfrBI ATG'CAT Cuts at: 2891 3094 2891 Size: 203 6422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 3024 3726 4042 3024 Size: 702 316 5607 Fragments arranged by size: 5607 702 316 Bme1580I G_kGCm'C Cuts at: 1790 3304 3672 4686 5842 1790 Size: 1514 368 1014 1156 2573 Fragments arranged by size: 2573 1514 1156 1014 368 BmgBI CAC'GTC Cuts at: 1318 2963 1318 Size: 1645 4980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2863 2908 4356 Size: 416 53 83 399 24 1446 45 1448 Cuts at: 4356 5314 442 Size: 958 1753 Fragments arranged by size: 1753 1448 1446 958 416 399 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3044 3044 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2823 3557 5285 1239 Size: 1584 734 1728 2579 Fragments arranged by size: 2579 1728 1584 734 Bpu10I CC'TnA_GC Cuts at: 2040 2493 2040 Size: 453 6172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2087 3732 4750 5615 5913 2087 Size: 1645 1018 865 298 2799 Fragments arranged by size: 2799 1645 1018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5288 882 Size: 617 3789 2219 Fragments arranged by size: 3789 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3881 4416 493 Size: 711 2677 535 2702 Fragments arranged by size: 2702 2677 711 535 BsaBI GATnn'nnATC Cuts at: 2610 3596 6619 2610 Size: 986 3023 2616 Fragments arranged by size: 3023 2616 986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3810 4432 4814 5449 Size: 53 83 186 936 2277 622 382 635 Cuts at: 5449 275 Size: 1451 Fragments arranged by size: 2277 1451 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2120 5058 5456 5730 5877 6288 2120 Size: 2938 398 274 147 411 2457 Fragments arranged by size: 2938 2457 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6400 Fragments arranged by size: 6400 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 1186 Size: 904 5721 BseYI C'CCAG_C Cuts at: 2429 3188 5828 6324 2429 Size: 759 2640 496 2730 Fragments arranged by size: 2730 2640 759 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3262 1163 Size: 573 1526 4526 Fragments arranged by size: 4526 1526 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2118 3030 3304 3727 4686 4771 5842 2100 Size: 18 912 274 423 959 85 1071 2883 Fragments arranged by size: 2883 1071 959 912 423 274 85 18 BsmI GAATG_Cn' Cuts at: 2350 2893 4136 4229 2350 Size: 543 1243 93 4746 Fragments arranged by size: 4746 1243 543 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3488 3488 Size: 6625 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2118 3030 3304 3672 3727 3944 Size: 399 310 18 912 274 368 55 217 Cuts at: 3944 4686 4771 5842 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 912 742 399 368 310 274 217 85 55 18 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 2292 4514 6244 2292 Size: 2222 1730 2673 Fragments arranged by size: 2673 2222 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 3980 844 Size: 2082 1054 3489 Fragments arranged by size: 3489 2082 1054 BsrBI CCG'CTC Cuts at: 1394 1823 4512 1394 Size: 429 2689 3507 Fragments arranged by size: 3507 2689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2554 3063 5120 5294 65 Size: 1183 1306 509 2057 174 1396 Fragments arranged by size: 2057 1396 1306 1183 509 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 3549 5269 5456 1700 Size: 110 310 1429 1720 187 2869 Fragments arranged by size: 2869 1720 1429 310 187 110 BsrGI T'GTAC_A Cuts at: 96 2522 96 Size: 2426 4199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2111 4685 5697 2111 Size: 2574 1012 3039 Fragments arranged by size: 3039 2574 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2091 2228 3185 794 Size: 1297 137 957 4234 Fragments arranged by size: 4234 1297 957 137 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 2594 2594 Size: 6625 BstYI r'GATC_y Cuts at: 1972 3307 3955 4322 4707 4724 6176 6262 6274 Size: 1335 648 367 385 17 1452 86 12 Cuts at: 6274 6620 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1335 648 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3086 3086 Size: 6625 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 3718 4141 4935 4955 3718 Size: 423 794 20 5388 Fragments arranged by size: 5388 794 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 2782 3929 2782 Size: 1147 5478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 4072 4961 Size: 54 1425 50 275 18 219 2023 889 Cuts at: 4961 6590 8 Size: 1629 43 Fragments arranged by size: 2023 1629 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 3480 3816 3891 4555 1353 Size: 1757 370 336 75 664 3423 Fragments arranged by size: 3423 1757 664 370 336 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2839 5212 5586 5732 387 Size: 1064 138 1250 2373 374 146 1280 Fragments arranged by size: 2373 1280 1250 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2098 3028 3725 2098 Size: 930 697 4998 Fragments arranged by size: 4998 930 697 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2526 2664 2823 2880 3557 3702 4688 Size: 393 894 138 159 57 677 145 986 Cuts at: 4688 5285 6072 6340 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 986 894 787 677 597 393 268 159 145 138 57 EcoNI CCTnn'n_nnAGG Cuts at: 3620 3620 Size: 6625 EcoO109I rG'GnC_Cy Cuts at: 3668 3668 Size: 6625 EcoRI G'AATT_C Cuts at: 3934 4312 3934 Size: 378 6247 EcoRV GAT'ATC Cuts at: 3130 3130 Size: 6625 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 5772 1678 Size: 4094 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3035 3067 3114 3119 3146 Size: 32 135 32 1463 32 47 5 27 Cuts at: 3146 3151 5266 5298 5340 5372 1373 Size: 5 2115 32 42 32 2626 Fragments arranged by size: 2626 2115 1463 135 47 42 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3969 4215 669 Size: 988 281 867 1164 246 3079 Fragments arranged by size: 3079 1164 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 2774 3284 748 Size: 2026 510 4089 Fragments arranged by size: 4089 2026 510 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 3322 4224 3322 Size: 902 5723 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2188 2347 2829 5739 5923 1305 Size: 883 159 482 2910 184 2007 Fragments arranged by size: 2910 2007 883 482 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4803 Fragments arranged by size: 4803 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4583 4942 5101 518 Size: 648 3417 359 159 2042 Fragments arranged by size: 3417 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2399 2429 4041 4718 5866 6111 1606 Size: 793 30 1612 677 1148 245 2120 Fragments arranged by size: 2120 1612 1148 793 677 245 30 NaeI GCC'GGC Cuts at: 3551 3551 Size: 6625 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 NdeI CA'TA_TG Cuts at: 387 3032 387 Size: 2645 3980 NgoMIV G'CCGG_C Cuts at: 3549 3549 Size: 6625 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NsiI A_TGCA'T Cuts at: 2893 3096 2893 Size: 203 6422 NspI r_CATG'y Cuts at: 1132 1229 2207 3983 5528 1132 Size: 97 978 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 978 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 2203 5524 2203 Size: 3321 3304 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6625 PfoI T'CCnGG_A Cuts at: 3573 3573 Size: 6625 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PspOMI G'GGCC_C Cuts at: 3668 3668 Size: 6625 PstI C_TGCA'G Cuts at: 830 2250 3977 830 Size: 1420 1727 3478 Fragments arranged by size: 3478 1727 1420 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2399 2429 2399 Size: 30 6595 SacI G_AGCT'C Cuts at: 2100 3030 3727 2100 Size: 930 697 4998 Fragments arranged by size: 4998 930 697 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 3891 3891 Size: 6625 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 2576 4873 6585 2576 Size: 2297 1712 2616 Fragments arranged by size: 2616 2297 1712 SfcI C'TryA_G Cuts at: 826 1045 2246 3973 5108 5789 5980 826 Size: 219 1201 1727 1135 681 191 1471 Fragments arranged by size: 1727 1471 1201 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 2030 3952 1872 Size: 158 1922 4545 Fragments arranged by size: 4545 1922 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2102 3747 4729 5630 5892 820 Size: 197 926 159 1645 982 901 262 1553 Fragments arranged by size: 1645 1553 982 926 901 262 197 159 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 2922 1517 Size: 1405 5220 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 3616 4064 513 Size: 552 939 228 1384 448 3074 Fragments arranged by size: 3074 1384 939 552 448 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 4652 4669 4822 5007 5452 325 Size: 1255 906 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 906 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2242 2522 2574 Size: 274 80 33 51 1443 265 280 52 Cuts at: 2574 2863 4871 6583 96 Size: 289 2008 1712 138 Fragments arranged by size: 2008 1712 1443 289 280 274 265 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3426 3458 6585 6617 3426 Size: 32 3127 32 3434 Fragments arranged by size: 3434 3127 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 2941 3961 2941 Size: 1020 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3452 3452 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 2028 3950 1870 Size: 158 1922 4545 Fragments arranged by size: 4545 1922 158 XmnI GAAnn'nnTTC Cuts at: 4754 4754 Size: 6625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3811 4433 276 Size: 53 83 186 936 2277 622 2468 Fragments arranged by size: 2468 2277 936 622 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BsiWI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI