(Circular) (Six-base) MAPSORT of: pFN21AE1016.seq Check: 4597 from: 1 to: 6025 pFN21AE1016 6025 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 3368 1937 Size: 1113 318 4594 Fragments arranged by size: 4594 1113 318 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 2721 4152 4525 1563 Size: 1158 1431 373 3063 Fragments arranged by size: 3063 1431 1158 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 2735 3155 4088 5472 5740 1632 Size: 424 679 420 933 1384 268 1917 Fragments arranged by size: 1917 1384 933 679 424 420 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2384 1184 Size: 1200 4825 AlwNI CAG_nnn'CTG Cuts at: 2079 2436 2553 2681 2871 2923 3173 3208 3768 Size: 357 117 128 190 52 250 35 560 Cuts at: 3768 5340 2079 Size: 1572 2764 Fragments arranged by size: 2764 1572 560 357 250 190 128 117 52 35 ApaLI G'TGCA_C Cuts at: 2369 4082 5238 2369 Size: 1713 1156 3156 Fragments arranged by size: 3156 1713 1156 ApoI r'AATT_y Cuts at: 1493 2707 3334 3560 3712 5821 1493 Size: 1214 627 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 627 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3350 1870 Size: 73 147 1260 4545 Fragments arranged by size: 4545 1260 147 73 BamHI G'GATC_C Cuts at: 2695 3355 3722 2695 Size: 660 367 4998 Fragments arranged by size: 4998 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2353 2523 2909 2964 3346 Size: 325 217 627 566 170 386 55 382 Cuts at: 3346 4801 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 566 386 382 325 217 170 55 BanII G_rGCy'C Cuts at: 1391 2627 3344 1391 Size: 1236 717 4072 Fragments arranged by size: 4072 1236 717 BbeI G_GCGC'C Cuts at: 2357 2913 2968 2357 Size: 556 55 5414 Fragments arranged by size: 5414 556 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 928 Size: 971 1122 3932 Fragments arranged by size: 3932 1122 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2757 4636 136 Size: 107 122 71 631 605 1085 1879 1525 Fragments arranged by size: 1879 1525 1085 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 2147 3442 2147 Size: 1295 4730 Bme1580I G_kGCm'C Cuts at: 1790 2373 2493 2526 4086 5242 1790 Size: 583 120 33 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 583 120 33 BmgBI CAC'GTC Cuts at: 1318 2630 1318 Size: 1312 4713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2557 3214 2557 Size: 657 5368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2641 2805 3047 4685 1239 Size: 1402 164 242 1638 2579 Fragments arranged by size: 2579 1638 1402 242 164 Bpu10I CC'TnA_GC Cuts at: 2346 2747 2346 Size: 401 5624 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4688 882 Size: 617 3189 2219 Fragments arranged by size: 3189 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2272 3816 493 Size: 711 1068 1544 2702 Fragments arranged by size: 2702 1544 1068 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2354 2910 2965 Size: 53 83 186 936 476 345 556 55 Cuts at: 2965 3832 4214 4849 275 Size: 867 382 635 1451 Fragments arranged by size: 1451 936 867 635 556 476 382 345 186 83 55 53 BsaWI w'CCGG_w Cuts at: 2559 4458 4856 5130 5277 5688 2559 Size: 1899 398 274 147 411 2896 Fragments arranged by size: 2896 1899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2614 2644 1178 Size: 30 165 30 1211 30 4559 Fragments arranged by size: 4559 1211 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2625 2855 3018 1186 Size: 1439 230 163 4193 Fragments arranged by size: 4193 1439 230 163 BseYI C'CCAG_C Cuts at: 2510 2882 5228 5724 2510 Size: 372 2346 496 2811 Fragments arranged by size: 2811 2346 496 372 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2449 2661 1163 Size: 573 713 212 4527 Fragments arranged by size: 4527 713 573 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2347 2373 2787 4086 4171 5242 2347 Size: 26 414 1299 85 1071 3130 Fragments arranged by size: 3130 1299 1071 414 85 26 BsmI GAATG_Cn' Cuts at: 2504 3536 3629 2504 Size: 1032 93 4900 Fragments arranged by size: 4900 1032 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2821 2821 Size: 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 2373 2493 2526 2627 2787 3344 Size: 399 557 26 120 33 101 160 557 Cuts at: 3344 4086 4171 5242 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 557 399 160 120 101 85 33 26 BspEI T'CCGG_A Cuts at: 2559 5688 2559 Size: 3129 2896 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3380 844 Size: 2536 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 3281 3912 1394 Size: 429 317 1141 631 3507 Fragments arranged by size: 3507 1141 631 429 317 BsrDI GCAATG_nn' Cuts at: 65 1248 2404 4520 4694 65 Size: 1183 1156 2116 174 1396 Fragments arranged by size: 2116 1396 1183 1156 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 4669 4856 1700 Size: 110 509 2350 187 2869 Fragments arranged by size: 2869 2350 509 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 2054 1925 Size: 129 5896 BssSI C'ACGA_G Cuts at: 2637 4085 5097 2637 Size: 1448 1012 3565 Fragments arranged by size: 3565 1448 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2436 2871 794 Size: 1642 435 3948 Fragments arranged by size: 3948 1642 435 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstXI CCAn_nnnn'nTGG Cuts at: 2619 2619 Size: 6025 BstYI r'GATC_y Cuts at: 1972 2695 3355 3722 4107 4124 5576 5662 5674 Size: 723 660 367 385 17 1452 86 12 Cuts at: 5674 6020 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 723 660 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2097 2970 2097 Size: 873 5152 BtgI C'CryG_G Cuts at: 513 1065 2004 2664 3179 3213 513 Size: 552 939 660 515 34 3325 Fragments arranged by size: 3325 939 660 552 515 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2223 2403 487 Size: 683 56 997 180 4109 Fragments arranged by size: 4109 997 683 180 56 BtsI GCAGTG_nn' Cuts at: 3541 4335 4355 3541 Size: 794 20 5211 Fragments arranged by size: 5211 794 20 ClaI AT'CG_AT Cuts at: 1554 2775 1554 Size: 1221 4804 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2875 2910 3021 3056 529 Size: 35 2311 35 111 35 3498 Fragments arranged by size: 3498 2311 111 35 35 35 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 2342 2342 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3025 5032 809 Size: 507 1709 2007 1802 Fragments arranged by size: 2007 1802 1709 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2259 2588 Size: 54 1425 50 275 18 327 102 329 Cuts at: 2588 3472 4361 5990 8 Size: 884 889 1629 43 Fragments arranged by size: 1629 1425 889 884 329 327 275 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2840 3955 1353 Size: 1487 1115 3423 Fragments arranged by size: 3423 1487 1115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2942 4612 4986 5132 387 Size: 1064 138 528 825 1670 374 146 1280 Fragments arranged by size: 1670 1280 1064 825 528 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2641 2735 2805 3047 3155 4088 Size: 393 424 585 94 70 242 108 933 Cuts at: 4088 4685 5472 5740 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 933 787 597 585 424 393 268 242 108 94 70 EcoNI CCTnn'n_nnAGG Cuts at: 2409 2531 2640 2409 Size: 122 109 5794 Fragments arranged by size: 5794 122 109 EcoO109I rG'GnC_Cy Cuts at: 2129 3122 2129 Size: 993 5032 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 EcoRV GAT'ATC Cuts at: 2773 2773 Size: 6025 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2965 2965 Size: 6025 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 2357 2913 2968 5172 1678 Size: 679 556 55 2204 2531 Fragments arranged by size: 2531 2204 679 556 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2614 2646 4666 4698 4740 Size: 32 135 32 1042 32 2020 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 2020 1042 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KasI G'GCGC_C Cuts at: 2353 2909 2964 2353 Size: 556 55 5414 Fragments arranged by size: 5414 556 55 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 5139 5323 1305 Size: 1662 2172 184 2007 Fragments arranged by size: 2172 2007 1662 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2590 10 Size: 54 1425 343 429 329 3445 Fragments arranged by size: 3445 1425 429 343 329 54 MslI CAynn'nnrTG Cuts at: 518 1166 2376 3983 4342 4501 518 Size: 648 1210 1607 359 159 2042 Fragments arranged by size: 2042 1607 1210 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2553 2741 2868 3181 3441 4118 5266 5511 Size: 947 188 127 313 260 677 1148 245 Cuts at: 5511 1606 Size: 2120 Fragments arranged by size: 2120 1148 947 677 313 260 245 188 127 NaeI GCC'GGC Cuts at: 2321 2321 Size: 6025 NarI GG'CG_CC Cuts at: 2354 2910 2965 2354 Size: 556 55 5414 Fragments arranged by size: 5414 556 55 NcoI C'CATG_G Cuts at: 513 1065 2004 3213 513 Size: 552 939 1209 3325 Fragments arranged by size: 3325 1209 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NgoMIV G'CCGG_C Cuts at: 2319 2319 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 3383 4928 1132 Size: 97 2154 1545 2229 Fragments arranged by size: 2229 2154 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PfoI T'CCnGG_A Cuts at: 2125 2125 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2129 3122 2129 Size: 993 5032 PshAI GACnn'nnGTC Cuts at: 1613 2813 1613 Size: 1200 4825 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PstI C_TGCA'G Cuts at: 830 2087 3258 3377 830 Size: 1257 1171 119 3478 Fragments arranged by size: 3478 1257 1171 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 2741 2868 2741 Size: 127 5898 SacII CC_GC'GG Cuts at: 3182 3182 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SanDI GG'GwC_CC Cuts at: 2129 3122 2129 Size: 993 5032 SapI GCTCTTCn'nnn_ Cuts at: 2840 2840 Size: 6025 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 2083 3051 3071 3254 3373 4508 5189 Size: 219 1038 968 20 183 119 1135 681 Cuts at: 5189 5380 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1038 968 681 219 191 183 119 20 SfoI GGC'GCC Cuts at: 2355 2911 2966 2355 Size: 556 55 5414 Fragments arranged by size: 5414 556 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2027 1517 Size: 510 5515 StyI C'CwwG_G Cuts at: 513 1065 2004 2949 3213 3464 513 Size: 552 939 945 264 251 3074 Fragments arranged by size: 3074 945 939 552 264 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4052 4069 4222 4407 4852 325 Size: 1255 2472 17 153 185 445 1498 Fragments arranged by size: 2472 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5985 6017 5985 Size: 32 5993 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2616 2920 2616 Size: 304 5721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI