Restriction map for
pFN21AE4538
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AspEI AspI AvaI
AvrII BbeI BbrPI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
HgaI Hin1I HincII HindII HpaI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1676 GGTACC G^GTACC
AccI 1528 GTCTAC GT^[AC][GT]AC
AccIII 812 TCCGGA T^CCGGA
AseI 1714 ATTAAT AT^TAAT
AsnI 1714 ATTAAT AT^TAAT
Asp718I 1676 GGTACC G^GTACC
AviII 308 TGCGCA TGC^GCA
BalI 779 TGGCCA TGG^CCA
BamHI 1752 GGATCC G^GATCC
BanI 1676 GGTACC G^G[CT][GA]CC
BbsI 607 GAAGAC GAAGAC(2/6)
BbvCI 967 GCTGAGG CCTCAGC(-5/-2)
BcgI 1568 GCAAGCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1444 CCGGG CC^[GC]GG
BpuAI 607 GAAGAC GAAGAC(2/6)
BsaAI 1454 CACGTA [CT]AC^GT[GA]
BsaBI 1120 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 812 TCCGGA [AT]^CCGG[AT]
BseAI 812 TCCGGA T^CCGGA
BsgI 237 GTGCAG GTGCAG(16/14)
BsmI 1502 GCATTC GAATGC(1/-1)
BspEI 812 TCCGGA T^CCGGA
DraIII 231 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 806 CCAAGG C^C[AT][AT]GG
EcoT22I 1312 ATGCAT ATGCA^T
FspI 308 TGCGCA TGC^GCA
KpnI 1676 GGTACC GGTAC^C
MaeII 1455 ACGT A^CGT
MamI 1120 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 779 TGGCCA TGG^CCA
MroI 812 TCCGGA T^CCGGA
MscI 779 TGGCCA TGG^CCA
NciI 1444 CCGGG CC^[GC]GG
NsiI 1312 ATGCAT ATGCA^T
PshBI 1714 ATTAAT AT^TAAT
PstI 43 CTGCAG CTGCA^G
PvuII 670 CAGCTG CAG^CTG
SapI 451 GAAGAGC GCTCTTC(1/4)
ScaI 908 AGTACT AGT^ACT
SfcI 43 CTGCAG C^T[GA][CT]AG
SspI 640 AATATT AAT^ATT
StyI 806 CCAAGG C^C[AT][AT]GG
Tsp45I 478 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 398 AAATTC [GA]^AATT[CT]
1279 AAATTC
AflIII 249 ACATGT A^C[GA][CT]GT
474 ACATGT
AlwNI 355 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1574 CAGTCGCTG
Aor51HI 819 AGCGCT AGC^GCT
1396 AGCGCT
ApoI 398 AAATTC [GA]^AATT[CT]
1279 AAATTC
Asp700I 459 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1109 GAAAAGCTTC
AspHI 733 GAGCTC G[AT]GC[AT]^C
1648 GAGCTC
BanII 733 GAGCTC G[GA]GC[CT]^C
1648 GAGCTC
BsiHKAI 733 GAGCTC G[AT]GC[AT]^C
1648 GAGCTC
BspDI 1127 ATCGAT AT^CGAT
1316 ATCGAT
BspLU11I 249 ACATGT A^CATGT
474 ACATGT
BstF5I 1662 CATCC GGATG(2/0)
1851 CATCC
BstYI 1119 AGATCC [GA]^GATC[CT]
1752 GGATCC
ClaI 1127 ATCGAT AT^CGAT
1316 ATCGAT
EaeI 779 TGGCCA [CT]^GGCC[GA]
1490 CGGCCA
Eco47III 819 AGCGCT AGC^GCT
1396 AGCGCT
FokI 1662 CATCC GGATG(9/13)
1851 CATCC
HaeII 819 AGCGCT [GA]GCGC^[CT]
1396 AGCGCT
HapII 813 CCGG C^CGG
1444 CCGG
HpaII 813 CCGG C^CGG
1444 CCGG
HphI 1266 GGTGA GGTGA(8/7)
60 TCACC
MflI 1119 AGATCC [GA]^GATC[CT]
1752 GGATCC
MseI 1021 TTAA T^TAA
1715 TTAA
MslI 312 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CACCATTATG
MspA1I 103 CAGCGG C[AC]G^C[GT]G
670 CAGCTG
MspI 813 CCGG C^CGG
1444 CCGG
NspI 249 ACATGT [GA]CATG^[CT]
474 ACATGT
PleI 191 GACTC GAGTC(4/5)
28 GAGTC
SacI 733 GAGCTC GAGCT^C
1648 GAGCTC
TfiI 90 GATTC G^A[AT]TC
1832 GAATC
Tru9I 1021 TTAA T^TAA
1715 TTAA
XhoII 1119 AGATCC [GA]^GATC[CT]
1752 GGATCC
XmnI 459 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1109 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HhaI HinP1I
HindIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TspRI TthHB8I