(Circular) (Six-base) MAPSORT of: pFN21AE4538.seq Check: 2803 from: 1 to: 6565 pFN21AE4538 6565 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3534 3908 1937 Size: 1597 374 4594 Fragments arranged by size: 4594 1597 374 Acc65I G'GTAC_C Cuts at: 3681 3886 3681 Size: 205 6360 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 2207 2387 2784 3105 3380 3697 3776 Size: 561 14 180 397 321 275 317 79 Cuts at: 3776 4628 6012 6280 1632 Size: 852 1384 268 1917 Fragments arranged by size: 1917 1384 852 561 397 321 317 275 268 180 79 14 AfeI AGC'GCT Cuts at: 2826 3403 2826 Size: 577 5988 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 2254 2479 5464 6441 1203 Size: 1051 225 2985 977 1327 Fragments arranged by size: 2985 1327 1051 977 225 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 5410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AlwNI CAG_nnn'CTG Cuts at: 2365 3584 4308 5880 2365 Size: 1219 724 1572 3050 Fragments arranged by size: 3050 1572 1219 724 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 2403 3284 3874 4100 4252 6361 1493 Size: 910 881 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 910 881 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 3720 5119 160 Size: 872 2688 1399 1606 Fragments arranged by size: 2688 1606 1399 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 3890 1870 Size: 73 1947 4545 Fragments arranged by size: 4545 1947 73 BamHI G'GATC_C Cuts at: 3757 3895 4262 3757 Size: 138 367 6060 Fragments arranged by size: 6060 367 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3681 3886 5341 618 Size: 325 217 627 1894 205 1455 1842 Fragments arranged by size: 1894 1842 1455 627 325 217 205 BanII G_rGCy'C Cuts at: 1391 2742 3657 3884 1391 Size: 1351 915 227 4072 Fragments arranged by size: 4072 1351 915 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2619 928 Size: 971 720 4874 Fragments arranged by size: 4874 971 720 BbvCI CC'TCA_GC Cuts at: 2973 2973 Size: 6565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3562 3596 4756 4790 1903 Size: 34 1625 34 1160 34 3678 Fragments arranged by size: 3678 1625 1160 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4457 5667 4457 Size: 1210 5355 BclI T'GATC_A Cuts at: 1548 1548 Size: 6565 BfrBI ATG'CAT Cuts at: 3319 3319 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5176 136 Size: 107 122 71 631 605 3504 1525 Fragments arranged by size: 3504 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 2448 4626 5782 1790 Size: 658 2178 1156 2573 Fragments arranged by size: 2573 2178 1156 658 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6565 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 4296 5254 Size: 416 53 83 399 24 1509 1370 958 Cuts at: 5254 442 Size: 1753 Fragments arranged by size: 1753 1509 1370 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3203 3203 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2981 3109 3173 3671 3686 5225 1239 Size: 1742 128 64 498 15 1539 2579 Fragments arranged by size: 2579 1742 1539 498 128 64 15 Bpu10I CC'TnA_GC Cuts at: 2973 2973 Size: 6565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4690 5555 5853 4690 Size: 865 298 5402 Fragments arranged by size: 5402 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5228 882 Size: 617 3729 2219 Fragments arranged by size: 3729 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3461 4356 493 Size: 711 2257 895 2702 Fragments arranged by size: 2702 2257 895 711 BsaBI GATnn'nnATC Cuts at: 3129 6559 3129 Size: 3430 3135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4372 4754 5389 275 Size: 53 83 186 936 2839 382 635 1451 Fragments arranged by size: 2839 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2817 4998 5396 5670 5817 6228 2817 Size: 2181 398 274 147 411 3154 Fragments arranged by size: 3154 2181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3516 3546 1178 Size: 30 165 30 2113 30 4197 Fragments arranged by size: 4197 2113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2045 2534 3162 3396 1186 Size: 837 22 489 628 234 4355 Fragments arranged by size: 4355 837 628 489 234 22 BseYI C'CCAG_C Cuts at: 2447 5768 6264 2447 Size: 3321 496 2748 Fragments arranged by size: 3321 2748 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2263 1163 Size: 573 527 5465 Fragments arranged by size: 5465 573 527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2742 3657 4626 4711 5782 2742 Size: 915 969 85 1071 3525 Fragments arranged by size: 3525 1071 969 915 85 BsmI GAATG_Cn' Cuts at: 3507 4076 4169 3507 Size: 569 93 5903 Fragments arranged by size: 5903 569 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2448 2742 3657 3884 4626 4711 5782 Size: 399 658 294 915 227 742 85 1071 Cuts at: 5782 1391 Size: 2174 Fragments arranged by size: 2174 1071 915 742 658 399 294 227 85 BspEI T'CCGG_A Cuts at: 2817 6228 2817 Size: 3411 3154 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3920 844 Size: 3076 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4452 1394 Size: 429 2629 3507 Fragments arranged by size: 3507 2629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5060 5234 65 Size: 1183 3812 174 1396 Fragments arranged by size: 3812 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5209 5396 1700 Size: 110 3399 187 2869 Fragments arranged by size: 3399 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 4625 5637 4625 Size: 1012 5553 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6565 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6565 BstYI r'GATC_y Cuts at: 1972 3124 3757 3895 4262 4647 4664 6116 6202 Size: 1152 633 138 367 385 17 1452 86 Cuts at: 6202 6214 6560 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1152 633 385 367 346 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6013 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5826 Fragments arranged by size: 5826 683 56 BtsI GCAGTG_nn' Cuts at: 2712 4081 4875 4895 2712 Size: 1369 794 20 4382 Fragments arranged by size: 4382 1369 794 20 ClaI AT'CG_AT Cuts at: 1554 3133 3322 1554 Size: 1579 189 4797 Fragments arranged by size: 4797 1579 189 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2880 2915 529 Size: 35 2316 35 4179 Fragments arranged by size: 4179 2316 35 35 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DraIII CAC_nnn'GTG Cuts at: 2241 2241 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5572 809 Size: 507 4256 1802 Fragments arranged by size: 4256 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2784 3495 4012 Size: 54 1425 50 275 18 954 711 517 Cuts at: 4012 4901 6530 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 954 889 711 517 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 2451 3210 4495 1353 Size: 816 282 759 1285 3423 Fragments arranged by size: 3423 1285 816 759 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 5152 5526 5672 387 Size: 1064 138 453 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 2740 3655 2740 Size: 915 5650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2207 2387 2784 2981 3105 3109 Size: 393 561 14 180 397 197 124 4 Cuts at: 3109 3173 3380 3671 3686 3697 3776 4628 5225 Size: 64 207 291 15 11 79 852 597 Cuts at: 5225 6012 6280 1239 Size: 787 268 1524 Fragments arranged by size: 1524 852 787 597 561 397 393 291 268 207 197 180 124 79 64 15 14 11 4 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3073 3073 Size: 6565 FspI TGC'GCA Cuts at: 2315 5071 2315 Size: 2756 3809 HaeII r_GCGC'y Cuts at: 1678 2828 3405 5712 1678 Size: 1150 577 2307 2531 Fragments arranged by size: 2531 2307 1150 577 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 3516 3548 5206 Size: 32 135 32 851 32 1061 32 1658 Cuts at: 5206 5238 5280 5312 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1658 1061 851 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3909 4155 669 Size: 988 281 1971 246 3079 Fragments arranged by size: 3079 1971 988 281 246 HindIII A'AGCT_T Cuts at: 748 2587 2952 3117 748 Size: 1839 365 165 4196 Fragments arranged by size: 4196 1839 365 165 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3685 3890 3685 Size: 205 6360 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2829 5679 5863 1305 Size: 1524 2850 184 2007 Fragments arranged by size: 2850 2007 1524 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 10 Size: 54 1425 343 954 3789 Fragments arranged by size: 3789 1425 954 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2792 4523 4882 5041 518 Size: 648 1155 471 1731 359 159 2042 Fragments arranged by size: 2042 1731 1155 648 471 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2677 3981 4658 5806 6051 1606 Size: 504 567 1304 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 677 567 504 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6013 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NsiI A_TGCA'T Cuts at: 3321 3321 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 2258 2483 3923 5468 1132 Size: 97 1029 225 1440 1545 2229 Fragments arranged by size: 2229 1545 1440 1029 225 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6565 PciI A'CATG_T Cuts at: 2254 2479 5464 2254 Size: 225 2985 3355 Fragments arranged by size: 3355 2985 225 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6565 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 6565 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2355 2387 2355 Size: 32 6533 PstI C_TGCA'G Cuts at: 830 2052 3917 830 Size: 1222 1865 3478 Fragments arranged by size: 3478 1865 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 2677 2677 Size: 6565 SacI G_AGCT'C Cuts at: 2742 3657 2742 Size: 915 5650 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SapI GCTCTTCn'nnn_ Cuts at: 2451 2451 Size: 6565 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 2915 4813 6525 2915 Size: 1898 1712 2955 Fragments arranged by size: 2955 1898 1712 SfcI C'TryA_G Cuts at: 826 1045 2048 3913 5048 5729 5920 826 Size: 219 1003 1865 1135 681 191 1471 Fragments arranged by size: 1865 1471 1135 1003 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 3892 1872 Size: 2020 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4669 5570 5832 820 Size: 197 926 2726 901 262 1553 Fragments arranged by size: 2726 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 2647 4489 5 Size: 47 2595 1842 2081 Fragments arranged by size: 2595 2081 1842 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6565 StyI C'CwwG_G Cuts at: 513 1065 2811 4004 513 Size: 552 1746 1193 3074 Fragments arranged by size: 3074 1746 1193 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 4592 4609 4762 4947 5392 325 Size: 1255 753 2259 17 153 185 445 1498 Fragments arranged by size: 2259 1498 1255 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2913 4811 6523 Size: 274 80 33 51 1443 936 1898 1712 Cuts at: 6523 96 Size: 138 Fragments arranged by size: 1898 1712 1443 936 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6525 6557 6525 Size: 32 6533 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 3890 1870 Size: 2020 4545 XmnI GAAnn'nnTTC Cuts at: 2468 3118 4694 2468 Size: 650 1576 4339 Fragments arranged by size: 4339 1576 650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI