Restriction map for
pFN21AE4538
(Linear) (Six-base) MAPSORT of: KIEE4538.seq Check: 878 from: 1 to: 1860
KIEE4538.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 0 1529 1860
Size: 1529 331
Acc65I G'GTAC_C
Cuts at: 0 1676 1860
Size: 1676 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 202 382 779 1100 1375 1692 1771
Size: 188 14 180 397 321 275 317 79
Cuts at: 1771 1860
Size: 89
Fragments arranged by size:
397 321 317 275 188 180 89 79
14
AfeI AGC'GCT
Cuts at: 0 821 1398 1860
Size: 821 577 462
Fragments arranged by size:
821 577 462
AflIII A'CryG_T
Cuts at: 0 249 474 1860
Size: 249 225 1386
Fragments arranged by size:
1386 249 225
AleI CACnn'nnGTG
Cuts at: 0 316 1860
Size: 316 1544
AlwNI CAG_nnn'CTG
Cuts at: 0 360 1579 1860
Size: 360 1219 281
Fragments arranged by size:
1219 360 281
ApoI r'AATT_y
Cuts at: 0 398 1279 1860
Size: 398 881 581
Fragments arranged by size:
881 581 398
AseI AT'TA_AT
Cuts at: 0 1715 1860
Size: 1715 145
BamHI G'GATC_C
Cuts at: 0 1752 1860
Size: 1752 108
BanI G'GyrC_C
Cuts at: 0 1676 1860
Size: 1676 184
BanII G_rGCy'C
Cuts at: 0 737 1652 1860
Size: 737 915 208
Fragments arranged by size:
915 737 208
BbsI GAAGACnn'nnnn_
Cuts at: 0 614 1860
Size: 614 1246
BbvCI CC'TCA_GC
Cuts at: 0 968 1860
Size: 968 892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1557 1591 1860
Size: 1557 34 269
Fragments arranged by size:
1557 269 34
BfrBI ATG'CAT
Cuts at: 0 1314 1860
Size: 1314 546
Bme1580I G_kGCm'C
Cuts at: 0 443 1860
Size: 443 1417
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1860
Size: 921 939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1198 1860
Size: 1198 662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1104 1168 1666 1681 1860
Size: 976 128 64 498 15 179
Fragments arranged by size:
976 498 179 128 64 15
Bpu10I CC'TnA_GC
Cuts at: 0 968 1860
Size: 968 892
BsaAI yAC'GTr
Cuts at: 0 1456 1860
Size: 1456 404
BsaBI GATnn'nnATC
Cuts at: 0 1124 1860
Size: 1124 736
BsaWI w'CCGG_w
Cuts at: 0 812 1860
Size: 812 1048
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1511 1541 1860
Size: 1511 30 319
Fragments arranged by size:
1511 319 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 40 529 1157 1391 1860
Size: 18 22 489 628 234 469
Fragments arranged by size:
628 489 469 234 22 18
BseYI C'CCAG_C
Cuts at: 0 442 1860
Size: 442 1418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1860
Size: 258 1602
BsiHKAI G_wGCw'C
Cuts at: 0 737 1652 1860
Size: 737 915 208
Fragments arranged by size:
915 737 208
BsmI GAATG_Cn'
Cuts at: 0 1502 1860
Size: 1502 358
Bsp1286I G_dGCh'C
Cuts at: 0 443 737 1652 1860
Size: 443 294 915 208
Fragments arranged by size:
915 443 294 208
BspEI T'CCGG_A
Cuts at: 0 812 1860
Size: 812 1048
BstYI r'GATC_y
Cuts at: 0 1119 1752 1860
Size: 1119 633 108
Fragments arranged by size:
1119 633 108
BtsI GCAGTG_nn'
Cuts at: 0 707 1860
Size: 707 1153
ClaI AT'CG_AT
Cuts at: 0 1128 1317 1860
Size: 1128 189 543
Fragments arranged by size:
1128 543 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 875 910 1860
Size: 875 35 950
Fragments arranged by size:
950 875 35
DraIII CAC_nnn'GTG
Cuts at: 0 236 1860
Size: 236 1624
EaeI y'GGCC_r
Cuts at: 0 779 1490 1860
Size: 779 711 370
Fragments arranged by size:
779 711 370
EarI CTCTTCn'nnn_
Cuts at: 0 164 446 1205 1860
Size: 164 282 759 655
Fragments arranged by size:
759 655 282 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1860
Size: 37 1823
EcoICRI GAG'CTC
Cuts at: 0 735 1650 1860
Size: 735 915 210
Fragments arranged by size:
915 735 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 202 382 779 976 1100 1104 1168
Size: 188 14 180 397 197 124 4 64
Cuts at: 1168 1375 1666 1681 1692 1771 1860
Size: 207 291 15 11 79 89
Fragments arranged by size:
397 291 207 197 188 180 124 89
79 64 15 14 11 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1068 1860
Size: 1068 792
FspI TGC'GCA
Cuts at: 0 310 1860
Size: 310 1550
HaeII r_GCGC'y
Cuts at: 0 823 1400 1860
Size: 823 577 460
Fragments arranged by size:
823 577 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 1511 1543 1860
Size: 418 32 1061 32 317
Fragments arranged by size:
1061 418 317 32 32
HindIII A'AGCT_T
Cuts at: 0 582 947 1112 1860
Size: 582 365 165 748
Fragments arranged by size:
748 582 365 165
KpnI G_GTAC'C
Cuts at: 0 1680 1860
Size: 1680 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1860
Size: 824 1036
MscI TGG'CCA
Cuts at: 0 781 1860
Size: 781 1079
MslI CAynn'nnrTG
Cuts at: 0 316 787 1860
Size: 316 471 1073
Fragments arranged by size:
1073 471 316
MspA1I CmG'CkG
Cuts at: 0 105 672 1860
Size: 105 567 1188
Fragments arranged by size:
1188 567 105
NsiI A_TGCA'T
Cuts at: 0 1316 1860
Size: 1316 544
NspI r_CATG'y
Cuts at: 0 253 478 1860
Size: 253 225 1382
Fragments arranged by size:
1382 253 225
PciI A'CATG_T
Cuts at: 0 249 474 1860
Size: 249 225 1386
Fragments arranged by size:
1386 249 225
PfoI T'CCnGG_A
Cuts at: 0 149 1860
Size: 149 1711
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 350 382 1860
Size: 350 32 1478
Fragments arranged by size:
1478 350 32
PstI C_TGCA'G
Cuts at: 0 47 1860
Size: 47 1813
PvuII CAG'CTG
Cuts at: 0 672 1860
Size: 672 1188
SacI G_AGCT'C
Cuts at: 0 737 1652 1860
Size: 737 915 208
Fragments arranged by size:
915 737 208
SapI GCTCTTCn'nnn_
Cuts at: 0 446 1860
Size: 446 1414
ScaI AGT'ACT
Cuts at: 0 910 1860
Size: 910 950
SfcI C'TryA_G
Cuts at: 0 43 1860
Size: 43 1817
SspI AAT'ATT
Cuts at: 0 642 1860
Size: 642 1218
StyI C'CwwG_G
Cuts at: 0 806 1860
Size: 806 1054
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 1860
Size: 328 1532
TatI w'GTAC_w
Cuts at: 0 908 1860
Size: 908 952
XmnI GAAnn'nnTTC
Cuts at: 0 463 1113 1860
Size: 463 650 747
Fragments arranged by size:
747 650 463
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApoI
AseI BamHI BanI BanII BbsI BbvCI BcgI BfrBI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BstYI BtsI ClaI CspCI DraIII EaeI EarI EciI
EcoICRI Eco57MI FalI FspI HaeII Hin4I HindIII KpnI
MmeI MscI MslI MspA1I NsiI NspI PciI PfoI
PsrI PstI PvuII SacI SapI ScaI SfcI SspI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BciVI BclI BglI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaHI BsiEI BsiWI BsmBI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI DraI DrdI EagI
EcoNI EcoO109I EcoRI EcoRV FseI FspAI HincII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI