################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccIII AclI AcyI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SfiI SfoI SfuI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 948 GGTACC G^GTACC AccII 1009 CGCG CG^CG Asp718I 948 GGTACC G^GTACC AspI 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 948 GGTACC G^G[CT][GA]CC BanII 80 GAGCTC G[GA]GC[CT]^C BbsI 442 GAAGAC GAAGAC(2/6) BbvCI 1497 CCTCAGC CCTCAGC(-5/-2) BcnI 722 CCCGG CC^[GC]GG BpmI 152 CTGGAG CTGGAG(16/14) BpuAI 442 GAAGAC GAAGAC(2/6) BseRI 821 CTCCTC GAGGAG(10/8) BsgI 1147 CTGCAC GTGCAG(16/14) BsmFI 301 GGGAC GGGAC(10/14) BspDI 529 ATCGAT AT^CGAT BsrDI 1386 CATTGC GCAATG(2/0) Bst1107I 981 GTATAC GTA^TAC BstUI 1009 CGCG CG^CG BstZ17I 981 GTATAC GTA^TAC ClaI 529 ATCGAT AT^CGAT EcoT14I 275 CCAAGG C^C[AT][AT]GG HphI 1086 TCACC GGTGA(8/7) KpnI 948 GGTACC GGTAC^C MaeII 328 ACGT A^CGT MaeIII 974 GTTAC ^GT[ACGT]AC MfeI 683 CAATTG C^AATTG MslI 1243 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 683 CAATTG C^AATTG MvnI 1009 CGCG CG^CG NaeI 69 GCCGGC GCC^GGC NciI 722 CCCGG CC^[GC]GG NgoMIV 69 GCCGGC G^CCGGC PaeR7I 1514 CTCGAG C^TCGAG PflFI 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 418 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1363 CTGCAG CTGCA^G SacI 80 GAGCTC GAGCT^C SalI 956 GTCGAC G^TCGAC SexAI 951 ACCTGGT A^CC[AT]GGT SmlI 1514 CTCGAG C^T[CT][GA]AG SpeI 168 ACTAGT A^CTAGT StyI 275 CCAAGG C^C[AT][AT]GG Tth111I 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1514 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AflIII 873 ACATGT A^C[GA][CT]GT 977 ACATGT AgeI 61 ACCGGT A^CCGGT 363 ACCGGT AlwI 400 GGATC GGATC(4/5) 1239 GGATC AlwNI 91 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 146 CAGTATCTG Asp700I 502 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 913 GAAGCACTTC AspHI 80 GAGCTC G[AT]GC[AT]^C 1491 GAGCAC BfaI 169 CTAG C^TAG 551 CTAG BglII 614 AGATCT A^GATCT 1127 AGATCT BsiHKAI 80 GAGCTC G[AT]GC[AT]^C 1491 GAGCAC BsmAI 1423 GAGAC GTCTC(1/5) 1517 GAGAC BspLU11I 873 ACATGT A^CATGT 977 ACATGT BstYI 614 AGATCT [GA]^GATC[CT] 1127 AGATCT DraII 423 AGGTCCT [GA]G^G[ACGT]CC[CT] 1395 GGGTCCT EaeI 437 TGGCCG [CT]^GGCC[GA] 524 CGGCCA EarI 943 GAAGAG CTCTTC(1/4) 1476 GAAGAG EcoO109I 423 AGGTCCT [GA]G^G[ACGT]CC[CT] 1395 GGGTCCT EcoRV 454 GATATC GAT^ATC 1078 GATATC FauI 9 GCGGG CCCGC(4/6) 18 GCGGG HaeII 3 GGCGCT [GA]GCGC^[CT] 93 GGCGCT HincII 28 GTCAAC GT[CT]^[GA]AC 956 GTCGAC HindII 28 GTCAAC GT[CT]^[GA]AC 956 GTCGAC Ksp632I 943 GAAGAG CTCTTC(1/4) 1476 GAAGAG MaeI 169 CTAG C^TAG 551 CTAG MflI 614 AGATCT [GA]^GATC[CT] 1127 AGATCT MseI 188 TTAA T^TAA 1201 TTAA MspA1I 842 CAGCTG C[AC]G^C[GT]G 1144 CAGCTG NspI 873 ACATGT [GA]CATG^[CT] 977 ACATGT PinAI 61 ACCGGT A^CCGGT 363 ACCGGT PleI 1519 GACTC GAGTC(4/5) 155 GAGTC PpuMI 423 AGGTCCT [GA]G^G[AT]CC[CT] 1395 GGGTCCT PvuII 842 CAGCTG CAG^CTG 1144 CAGCTG SfaNI 15 GATGC GCATC(5/9) 316 GATGC SfcI 663 CTACAG C^T[GA][CT]AG 1363 CTGCAG SgrAI 60 CACCGGTG C[GA]^CCGG[CT]G 362 CACCGGTG Tru9I 188 TTAA T^TAA 1201 TTAA TspRI 666 CAGTG CA[GC]TG[ACGT][ACGT]^ 1413 CACTG XhoII 614 AGATCT [GA]^GATC[CT] 1127 AGATCT XmnI 502 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 913 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccI AciI AcsI AfaI AluI ApoI AvaI AvaII BbvI BmyI BsaJI BsaWI BsiYI BslI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp509I TthHB8I