(Circular) (Six-base) MAPSORT of: pFN21AE4489.seq Check: 7589 from: 1 to: 6238 pFN21AE4489 6238 bp With 182 enzymes: SgfI * March 28, 2012 13:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 2962 2987 3581 1937 Size: 727 298 25 594 4594 Fragments arranged by size: 4594 727 594 298 25 Acc65I G'GTAC_C Cuts at: 2953 3559 2953 Size: 606 5632 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2295 2937 3500 4301 5685 5953 1632 Size: 663 642 563 801 1384 268 1917 Fragments arranged by size: 1917 1384 801 663 642 563 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 2878 2982 5137 6114 1203 Size: 1675 104 2155 977 1327 Fragments arranged by size: 2155 1675 1327 977 104 AgeI A'CCGG_T Cuts at: 2066 2368 5069 2066 Size: 302 2701 3235 Fragments arranged by size: 3235 2701 302 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 2108 Size: 32 6206 AlwNI CAG_nnn'CTG Cuts at: 2101 2156 3981 5553 2101 Size: 55 1825 1572 2786 Fragments arranged by size: 2786 1825 1572 55 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2568 2680 2852 3362 3509 3547 3773 3925 Size: 1075 112 172 510 147 38 226 152 Cuts at: 3925 6034 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1075 510 226 172 152 147 112 38 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2088 3519 3563 1870 Size: 73 105 40 1431 44 4545 Fragments arranged by size: 4545 1431 105 73 44 40 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2953 3559 5014 618 Size: 325 217 627 1166 606 1455 1842 Fragments arranged by size: 1842 1455 1166 627 606 325 217 BanII G_rGCy'C Cuts at: 1391 2089 3557 1391 Size: 698 1468 4072 Fragments arranged by size: 4072 1468 698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 928 Size: 971 555 4712 Fragments arranged by size: 4712 971 555 BbvCI CC'TCA_GC Cuts at: 3503 3503 Size: 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2619 3132 6233 2619 Size: 513 3101 2624 Fragments arranged by size: 3101 2624 513 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 2064 4299 5455 1790 Size: 274 2235 1156 2573 Fragments arranged by size: 2573 2235 1156 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2358 3969 4927 Size: 416 53 83 399 24 941 1611 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1753 1611 958 941 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3507 3507 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 4898 1239 Size: 939 2720 2579 Fragments arranged by size: 2720 2579 939 Bpu10I CC'TnA_GC Cuts at: 3396 3503 3396 Size: 107 6131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4901 882 Size: 617 3402 2219 Fragments arranged by size: 3402 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 4427 5062 275 Size: 53 83 186 936 2512 382 635 1451 Fragments arranged by size: 2512 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2066 2233 2368 4671 5069 5343 5490 5901 2066 Size: 167 135 2303 398 274 147 411 2403 Fragments arranged by size: 2403 2303 411 398 274 167 147 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 1178 Size: 30 165 30 705 30 5278 Fragments arranged by size: 5278 705 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2817 1186 Size: 1631 4607 BseYI C'CCAG_C Cuts at: 2127 2588 3017 5441 5937 2127 Size: 461 429 2424 496 2428 Fragments arranged by size: 2428 2424 496 461 429 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3137 1163 Size: 573 1401 4264 Fragments arranged by size: 4264 1401 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2089 3500 4299 4384 5455 2089 Size: 1411 799 85 1071 2872 Fragments arranged by size: 2872 1411 1071 799 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2089 3500 3557 4299 4384 5455 Size: 399 274 25 1411 57 742 85 1071 Cuts at: 5455 1391 Size: 2174 Fragments arranged by size: 2174 1411 1071 742 399 274 85 57 25 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3593 844 Size: 2749 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 4733 4907 65 Size: 1183 2142 1343 174 1396 Fragments arranged by size: 2142 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2074 2368 4882 5069 1700 Size: 110 256 8 294 2514 187 2869 Fragments arranged by size: 2869 2514 294 256 187 110 8 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2619 3132 3568 3935 4320 4337 5789 5875 Size: 647 513 436 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 647 513 436 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2988 2988 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4747 Fragments arranged by size: 4747 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2033 487 Size: 683 56 807 4692 Fragments arranged by size: 4692 807 683 56 BtsI GCAGTG_nn' Cuts at: 3754 4548 4568 3754 Size: 794 20 5424 Fragments arranged by size: 5424 794 20 ClaI AT'CG_AT Cuts at: 1554 2535 1554 Size: 981 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2529 3685 Size: 54 1425 50 275 18 612 87 1156 Cuts at: 3685 4574 6203 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1156 889 612 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2943 3476 4168 1353 Size: 1590 533 692 3423 Fragments arranged by size: 3423 1590 692 533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2087 2087 Size: 6238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2295 2937 3500 4301 4898 5685 Size: 393 546 117 642 563 801 597 787 Cuts at: 5685 5953 1239 Size: 268 1524 Fragments arranged by size: 1524 801 787 642 597 563 546 393 268 117 EcoO109I rG'GnC_Cy Cuts at: 2429 3401 2429 Size: 972 5266 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 EcoRV GAT'ATC Cuts at: 2461 3085 2461 Size: 624 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2471 3350 2471 Size: 879 5359 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2012 2102 5385 1678 Size: 334 90 3283 2531 Fragments arranged by size: 3283 2531 334 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2170 2202 2599 2631 4879 Size: 32 135 32 598 32 397 32 2248 Cuts at: 4879 4911 4953 4985 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2248 598 397 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2035 2963 3582 3828 669 Size: 988 281 97 928 619 246 3079 Fragments arranged by size: 3079 988 928 619 281 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 2957 3563 2957 Size: 606 5632 MfeI C'AATT_G Cuts at: 2688 3837 2688 Size: 1149 5089 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2093 3301 5352 5536 1305 Size: 788 1208 2051 184 2007 Fragments arranged by size: 2051 2007 1208 788 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4416 Fragments arranged by size: 4416 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3252 4196 4555 4714 518 Size: 648 2086 944 359 159 2042 Fragments arranged by size: 2086 2042 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2849 3151 3654 4331 5479 5724 1606 Size: 1243 302 503 677 1148 245 2120 Fragments arranged by size: 2120 1243 1148 677 503 302 245 NaeI GCC'GGC Cuts at: 2076 2076 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4747 Fragments arranged by size: 4747 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 2882 2986 3539 3596 5141 1132 Size: 97 1653 104 553 57 1545 2229 Fragments arranged by size: 2229 1653 1545 553 104 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 2878 2982 5137 2878 Size: 104 2155 3979 Fragments arranged by size: 3979 2155 104 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 4307 4339 5820 5852 2108 Size: 32 2167 32 1481 32 2494 Fragments arranged by size: 2494 2167 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 3401 2429 Size: 972 5266 PshAI GACnn'nnGTC Cuts at: 1613 2427 1613 Size: 814 5424 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3372 3590 830 Size: 2542 218 3478 Fragments arranged by size: 3478 2542 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2849 3151 2849 Size: 302 5936 SacI G_AGCT'C Cuts at: 2089 2089 Size: 6238 SalI G'TCGA_C Cuts at: 1936 2961 3580 1936 Size: 1025 619 4594 Fragments arranged by size: 4594 1025 619 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SexAI A'CCwGG_T Cuts at: 2956 2956 Size: 6238 SfcI C'TryA_G Cuts at: 826 1045 2668 3368 3586 4721 5402 5593 826 Size: 219 1623 700 218 1135 681 191 1471 Fragments arranged by size: 1623 1471 1135 700 681 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 2066 2368 1700 Size: 366 302 5570 Fragments arranged by size: 5570 366 302 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3519 4342 5243 5505 820 Size: 197 926 1576 823 901 262 1553 Fragments arranged by size: 1576 1553 926 901 823 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 2173 152 Size: 2021 4217 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6238 StyI C'CwwG_G Cuts at: 513 1065 2004 2280 3677 513 Size: 552 939 276 1397 3074 Fragments arranged by size: 3074 1397 939 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 4265 4282 4435 4620 5065 325 Size: 1255 1116 1569 17 153 185 445 1498 Fragments arranged by size: 1569 1498 1255 1116 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2437 4484 6196 Size: 274 80 33 51 1443 460 2047 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 2047 1712 1443 460 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3282 3314 6198 6230 3282 Size: 32 2884 32 3290 Fragments arranged by size: 3290 2884 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2771 1316 Size: 1455 4783 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 3519 1943 Size: 1576 4662 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 2511 2922 4367 2511 Size: 411 1445 4382 Fragments arranged by size: 4382 1445 411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI