(Linear) (Six-base) MAPSORT of: KIEE4489.seq Check: 5785 from: 1 to: 1533 KIEE4489.orf With 182 enzymes: SgfI * March 22, 2012 18:11 .. AccI GT'mk_AC Cuts at: 0 659 957 982 1533 Size: 659 298 25 551 Fragments arranged by size: 659 551 298 25 Acc65I G'GTAC_C Cuts at: 0 948 1533 Size: 948 585 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 932 1495 1533 Size: 290 642 563 38 Fragments arranged by size: 642 563 290 38 AflIII A'CryG_T Cuts at: 0 873 977 1533 Size: 873 104 556 Fragments arranged by size: 873 556 104 AgeI A'CCGG_T Cuts at: 0 61 363 1533 Size: 61 302 1170 Fragments arranged by size: 1170 302 61 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 103 135 1533 Size: 103 32 1398 Fragments arranged by size: 1398 103 32 AlwNI CAG_nnn'CTG Cuts at: 0 96 151 1533 Size: 96 55 1382 Fragments arranged by size: 1382 96 55 ApoI r'AATT_y Cuts at: 0 563 675 847 1357 1504 1533 Size: 563 112 172 510 147 29 Fragments arranged by size: 563 510 172 147 112 29 AvaI C'yCGr_G Cuts at: 0 43 83 1514 1533 Size: 43 40 1431 19 Fragments arranged by size: 1431 43 40 19 BanI G'GyrC_C Cuts at: 0 948 1533 Size: 948 585 BanII G_rGCy'C Cuts at: 0 84 1533 Size: 84 1449 BbsI GAAGACnn'nnnn_ Cuts at: 0 449 1533 Size: 449 1084 BbvCI CC'TCA_GC Cuts at: 0 1498 1533 Size: 1498 35 BglII A'GATC_T Cuts at: 0 614 1127 1533 Size: 614 513 406 Fragments arranged by size: 614 513 406 Bme1580I G_kGCm'C Cuts at: 0 59 1533 Size: 59 1474 BmrI ACTGGGnnnn_n' Cuts at: 0 353 1533 Size: 353 1180 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1502 1533 Size: 1502 31 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 1533 Size: 173 1360 Bpu10I CC'TnA_GC Cuts at: 0 1391 1498 1533 Size: 1391 107 35 Fragments arranged by size: 1391 107 35 BsaWI w'CCGG_w Cuts at: 0 61 228 363 1533 Size: 61 167 135 1170 Fragments arranged by size: 1170 167 135 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 103 133 1533 Size: 103 30 1400 Fragments arranged by size: 1400 103 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 812 1533 Size: 812 721 BseYI C'CCAG_C Cuts at: 0 122 583 1012 1533 Size: 122 461 429 521 Fragments arranged by size: 521 461 429 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 1533 Size: 1132 401 BsiHKAI G_wGCw'C Cuts at: 0 84 1495 1533 Size: 84 1411 38 Fragments arranged by size: 1411 84 38 Bsp1286I G_dGCh'C Cuts at: 0 59 84 1495 1533 Size: 59 25 1411 38 Fragments arranged by size: 1411 59 38 25 BsrDI GCAATG_nn' Cuts at: 0 1385 1533 Size: 1385 148 BsrFI r'CCGG_y Cuts at: 0 61 69 363 1533 Size: 61 8 294 1170 Fragments arranged by size: 1170 294 61 8 BstYI r'GATC_y Cuts at: 0 614 1127 1533 Size: 614 513 406 Fragments arranged by size: 614 513 406 BstZ17I GTA'TAC Cuts at: 0 983 1533 Size: 983 550 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 28 1533 Size: 28 1505 ClaI AT'CG_AT Cuts at: 0 530 1533 Size: 530 1003 EaeI y'GGCC_r Cuts at: 0 437 524 1533 Size: 437 87 1009 Fragments arranged by size: 1009 437 87 EarI CTCTTCn'nnn_ Cuts at: 0 938 1471 1533 Size: 938 533 62 Fragments arranged by size: 938 533 62 EcoICRI GAG'CTC Cuts at: 0 82 1533 Size: 82 1451 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 290 932 1495 1533 Size: 173 117 642 563 38 Fragments arranged by size: 642 563 173 117 38 EcoO109I rG'GnC_Cy Cuts at: 0 424 1396 1533 Size: 424 972 137 Fragments arranged by size: 972 424 137 EcoRV GAT'ATC Cuts at: 0 456 1080 1533 Size: 456 624 453 Fragments arranged by size: 624 456 453 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 466 1345 1533 Size: 466 879 188 Fragments arranged by size: 879 466 188 HaeII r_GCGC'y Cuts at: 0 7 97 1533 Size: 7 90 1436 Fragments arranged by size: 1436 90 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 165 197 594 626 1533 Size: 165 32 397 32 907 Fragments arranged by size: 907 397 165 32 32 HincII GTy'rAC Cuts at: 0 30 958 1533 Size: 30 928 575 Fragments arranged by size: 928 575 30 KpnI G_GTAC'C Cuts at: 0 952 1533 Size: 952 581 MfeI C'AATT_G Cuts at: 0 683 1533 Size: 683 850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1296 1533 Size: 88 1208 237 Fragments arranged by size: 1208 237 88 MslI CAynn'nnrTG Cuts at: 0 1247 1533 Size: 1247 286 MspA1I CmG'CkG Cuts at: 0 844 1146 1533 Size: 844 302 387 Fragments arranged by size: 844 387 302 NaeI GCC'GGC Cuts at: 0 71 1533 Size: 71 1462 NgoMIV G'CCGG_C Cuts at: 0 69 1533 Size: 69 1464 NspI r_CATG'y Cuts at: 0 877 981 1533 Size: 877 104 552 Fragments arranged by size: 877 552 104 PciI A'CATG_T Cuts at: 0 873 977 1533 Size: 873 104 556 Fragments arranged by size: 873 556 104 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 1533 Size: 103 32 1398 Fragments arranged by size: 1398 103 32 PpuMI rG'GwC_Cy Cuts at: 0 424 1396 1533 Size: 424 972 137 Fragments arranged by size: 972 424 137 PshAI GACnn'nnGTC Cuts at: 0 422 1533 Size: 422 1111 PstI C_TGCA'G Cuts at: 0 1367 1533 Size: 1367 166 PvuII CAG'CTG Cuts at: 0 844 1146 1533 Size: 844 302 387 Fragments arranged by size: 844 387 302 SacI G_AGCT'C Cuts at: 0 84 1533 Size: 84 1449 SalI G'TCGA_C Cuts at: 0 956 1533 Size: 956 577 SexAI A'CCwGG_T Cuts at: 0 951 1533 Size: 951 582 SfcI C'TryA_G Cuts at: 0 663 1363 1533 Size: 663 700 170 Fragments arranged by size: 700 663 170 SgrAI Cr'CCGG_yG Cuts at: 0 61 363 1533 Size: 61 302 1170 Fragments arranged by size: 1170 302 61 SmlI C'TyrA_G Cuts at: 0 1514 1533 Size: 1514 19 SpeI A'CTAG_T Cuts at: 0 168 1533 Size: 168 1365 StyI C'CwwG_G Cuts at: 0 275 1533 Size: 275 1258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 691 1533 Size: 691 842 TatI w'GTAC_w Cuts at: 0 432 1533 Size: 432 1101 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1277 1309 1533 Size: 1277 32 224 Fragments arranged by size: 1277 224 32 Tth111I GACn'n_nGTC Cuts at: 0 766 1533 Size: 766 767 XhoI C'TCGA_G Cuts at: 0 1514 1533 Size: 1514 19 XmnI GAAnn'nnTTC Cuts at: 0 506 917 1533 Size: 506 411 616 Fragments arranged by size: 616 506 411 Enzymes that do cut: AccI Acc65I AcuI AflIII AgeI AloI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BstYI BstZ17I BtgZI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I NaeI NgoMIV NspI PciI PpiI PpuMI PshAI PstI PvuII SacI SalI SexAI SfcI SgrAI SmlI SpeI StyI TaqII TatI TstI Tth111I XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KasI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI SfiI SfoI SgfI SmaI SnaBI SphI SrfI SspI StuI SwaI XbaI XcmI XmaI ZraI