Restriction map for
pFN21AE3584
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BbeI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsiEI BsiHKAI
BsmI BsoBI BspDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeI MfeI MluNI
MscI MseI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 144 CGCG CG^CG
AccIII 1157 TCCGGA T^CCGGA
AcsI 333 AAATTT [GA]^AATT[CT]
AlwNI 1093 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 333 AAATTT [GA]^AATT[CT]
Asp700I 475 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 382 GGATCC G^GATCC
BanII 1109 GAGCCC G[GA]GC[CT]^C
BbvCI 1081 GCTGAGG CCTCAGC(-5/-2)
BcgI 213 CGATGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1245 GTATCC GTATCC(6/5)
BclI 257 TGATCA T^GATCA
BsaBI 280 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1157 TCCGGA [AT]^CCGG[AT]
BseAI 1157 TCCGGA T^CCGGA
BsiWI 1190 CGTACG C^GTACG
BsmBI 1056 CGTCTC CGTCTC(1/5)
Bsp1407I 518 TGTACA T^GTACA
BspEI 1157 TCCGGA T^CCGGA
BspHI 941 TCATGA T^CATGA
BspLU11I 515 ACATGT A^CATGT
BsrBI 1114 CCGCTC CCGCTC(-3/-3)
BsrGI 518 TGTACA T^GTACA
BstAPI 616 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 144 CGCG CG^CG
DraIII 85 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 1113 CCCGC CCCGC(4/6)
FbaI 257 TGATCA T^GATCA
MamI 280 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 143 ACGCGT A^CGCGT
MroI 1157 TCCGGA T^CCGGA
MvnI 144 CGCG CG^CG
NspI 515 ACATGT [GA]CATG^[CT]
PpuMI 491 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1128 CAGCTG CAG^CTG
RcaI 941 TCATGA T^CATGA
SexAI 767 ACCTGGT A^CC[AT]GGT
SfcI 56 CTATAG C^T[GA][CT]AG
SspBI 518 TGTACA T^GTACA
TfiI 180 GATTC G^A[AT]TC
XmnI 475 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BbrPI 418 CACGTG CAC^GTG
1279 CACGTG
BbsI 232 GAAGAC GAAGAC(2/6)
715 GAAGAC
BglII 477 AGATCT A^GATCT
732 AGATCT
BmyI 1109 GAGCCC G[AGT]GC[ACT]^C
1123 GGGCAC
BpuAI 232 GAAGAC GAAGAC(2/6)
715 GAAGAC
BsaI 502 GAGACC GGTCTC(1/5)
745 GAGACC
BseRI 976 GAGGAG GAGGAG(10/8)
997 GAGGAG
BsgI 751 CTGCAC GTGCAG(16/14)
1150 CTGCAC
Bsp1286I 1109 GAGCCC G[AGT]GC[ACT]^C
1123 GGGCAC
BspMI 590 ACCTGC ACCTGC(4/8)
103 GCAGGT
BsrDI 219 CATTGC GCAATG(2/0)
173 GCAATG
BstXI 677 CCACGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
965 CCAATGATCTGG
DdeI 25 CTCAG C^T[ACGT]AG
1082 CTGAG
DraII 491 AGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
DsaI 441 CCATGG C^C[GA][CT]GG
1271 CCATGG
EarI 399 GAAGAG CTCTTC(1/4)
669 GAAGAG
EcoO109I 491 AGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
HincII 703 GTCAAC GT[CT]^[GA]AC
1225 GTCAAC
HindII 703 GTCAAC GT[CT]^[GA]AC
1225 GTCAAC
Ksp632I 399 GAAGAG CTCTTC(1/4)
669 GAAGAG
MspA1I 1006 CAGCGG C[AC]G^C[GT]G
1128 CAGCTG
NcoI 441 CCATGG C^CATGG
1271 CCATGG
PmaCI 418 CACGTG CAC^GTG
1279 CACGTG
PmlI 418 CACGTG CAC^GTG
1279 CACGTG
Tsp45I 895 GTCAC ^GT[GC]AC
1292 GTCAC
XcmI 441 CCATGGATCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1278 CCACGTGTGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanI BbvI BpmI BsaAI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI XhoII