(Circular) (Six-base) MAPSORT of: pFN21AE3584.seq Check: 9766 from: 1 to: 6004 pFN21AE3584 6004 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2604 2604 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2256 2423 4067 5451 5719 1632 Size: 462 162 167 1644 1384 268 1917 Fragments arranged by size: 1917 1644 1384 462 268 167 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2148 2424 2520 3285 4903 5880 1203 Size: 945 276 96 765 1618 977 1327 Fragments arranged by size: 1618 1327 977 945 765 276 96 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2167 3039 1184 Size: 983 872 4149 Fragments arranged by size: 4149 983 872 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2411 2443 2411 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 3103 3747 5319 3103 Size: 644 1572 3788 Fragments arranged by size: 3788 1572 644 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2338 3313 3539 3691 5800 1493 Size: 845 975 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 845 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3329 1870 Size: 73 1386 4545 Fragments arranged by size: 4545 1386 73 BamHI G'GATC_C Cuts at: 2387 3334 3701 2387 Size: 947 367 4690 Fragments arranged by size: 4690 947 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2136 3030 3325 4780 Size: 325 217 627 264 85 894 295 1455 Cuts at: 4780 618 Size: 1842 Fragments arranged by size: 1842 1455 894 627 325 295 264 217 85 BanII G_rGCy'C Cuts at: 1391 3118 3323 1391 Size: 1727 205 4072 Fragments arranged by size: 4072 1727 205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2727 928 Size: 971 345 483 4205 Fragments arranged by size: 4205 971 483 345 BbvCI CC'TCA_GC Cuts at: 3087 3087 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2207 2241 4195 4229 1903 Size: 34 270 34 1954 34 3678 Fragments arranged by size: 3678 1954 270 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3261 3896 5106 3261 Size: 635 1210 4159 Fragments arranged by size: 4159 1210 635 BclI T'GATC_A Cuts at: 1548 2262 1548 Size: 714 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2482 2737 5999 2482 Size: 255 3262 2487 Fragments arranged by size: 3262 2487 255 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 3132 4065 5221 1790 Size: 1342 933 1156 2573 Fragments arranged by size: 2573 1342 1156 933 BmgBI CAC'GTC Cuts at: 1318 2731 2803 1318 Size: 1413 72 4519 Fragments arranged by size: 4519 1413 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 3735 4693 Size: 416 53 83 399 24 684 1634 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1634 958 684 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3137 3137 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 2337 2880 2999 4664 1239 Size: 996 102 543 119 1665 2579 Fragments arranged by size: 2579 1665 996 543 119 102 Bpu10I CC'TnA_GC Cuts at: 3087 3087 Size: 6004 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 2744 4667 882 Size: 617 1002 243 1923 2219 Fragments arranged by size: 2219 1923 1002 617 243 BsaAI yAC'GTr Cuts at: 493 1204 2425 2685 3195 3223 3286 3795 493 Size: 711 1221 260 510 28 63 509 2702 Fragments arranged by size: 2702 1221 711 510 509 260 63 28 BsaBI GATnn'nnATC Cuts at: 2289 5998 2289 Size: 3709 2295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3162 4437 4835 5109 5256 5667 3162 Size: 1275 398 274 147 411 3499 Fragments arranged by size: 3499 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2310 2340 3170 3200 1178 Size: 30 165 30 907 30 830 30 3982 Fragments arranged by size: 3982 907 830 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2996 3017 1186 Size: 1810 21 4173 Fragments arranged by size: 4173 1810 21 BseYI C'CCAG_C Cuts at: 2921 5207 5703 2921 Size: 2286 496 3222 Fragments arranged by size: 3222 2286 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2741 3140 1163 Size: 573 1005 399 4027 Fragments arranged by size: 4027 1005 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4065 4150 5221 4065 Size: 85 1071 4848 Fragments arranged by size: 4848 1071 85 BsiWI C'GTAC_G Cuts at: 3195 3195 Size: 6004 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 3067 3067 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3118 3132 3323 4065 4150 5221 1391 Size: 399 1328 14 191 742 85 1071 2174 Fragments arranged by size: 2174 1328 1071 742 399 191 85 14 BspEI T'CCGG_A Cuts at: 3162 5667 3162 Size: 2505 3499 BspHI T'CATG_A Cuts at: 2946 3893 5623 2946 Size: 947 1730 3327 Fragments arranged by size: 3327 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2604 3359 844 Size: 1255 505 755 3489 Fragments arranged by size: 3489 1255 755 505 BsrBI CCG'CTC Cuts at: 1394 1823 3121 3891 1394 Size: 429 1298 770 3507 Fragments arranged by size: 3507 1298 770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 2223 4499 4673 65 Size: 1183 937 38 2276 174 1396 Fragments arranged by size: 2276 1396 1183 937 174 38 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 2523 96 Size: 2427 3577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2627 794 Size: 1833 4171 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2977 2689 Size: 288 5716 BstYI r'GATC_y Cuts at: 1972 2387 2450 2482 2737 3006 3334 3701 4086 Size: 415 63 32 255 269 328 367 385 Cuts at: 4086 4103 5555 5641 5653 5999 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 415 385 367 346 328 269 255 86 63 32 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 3276 513 Size: 552 939 442 830 3241 Fragments arranged by size: 3241 939 830 552 442 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2376 487 Size: 683 56 1150 4115 Fragments arranged by size: 4115 1150 683 56 BtsI GCAGTG_nn' Cuts at: 2379 3169 3520 4314 4334 2379 Size: 790 351 794 20 4049 Fragments arranged by size: 4049 794 790 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DraIII CAC_nnn'GTG Cuts at: 2095 2095 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969 Size: 54 1425 50 275 18 1621 889 1629 Cuts at: 5969 8 Size: 43 Fragments arranged by size: 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 2669 3934 1353 Size: 1046 270 1265 3423 Fragments arranged by size: 3423 1265 1046 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2235 2256 2337 2423 2880 2999 Size: 393 462 141 21 81 86 457 119 Cuts at: 2999 4067 4664 5451 5719 1239 Size: 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 787 597 462 457 393 268 141 119 86 81 21 EcoO109I rG'GnC_Cy Cuts at: 2497 3148 2497 Size: 651 5353 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 2564 2596 2663 Size: 32 135 32 863 32 97 32 67 Cuts at: 2663 2695 4645 4677 4719 4751 1373 Size: 32 1950 32 42 32 2626 Fragments arranged by size: 2626 1950 863 135 97 67 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2710 3232 3348 3594 669 Size: 988 281 772 522 116 246 3079 Fragments arranged by size: 3079 988 772 522 281 246 116 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 2148 5880 2148 Size: 3732 2272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 2840 2945 3021 3962 4321 4480 Size: 648 1012 662 105 76 941 359 159 Cuts at: 4480 518 Size: 2042 Fragments arranged by size: 2042 1012 941 662 648 359 159 105 76 MspA1I CmG'CkG Cuts at: 1606 3013 3135 3420 4097 5245 5490 1606 Size: 1407 122 285 677 1148 245 2120 Fragments arranged by size: 2120 1407 1148 677 285 245 122 NcoI C'CATG_G Cuts at: 513 1065 2004 2446 3276 513 Size: 552 939 442 830 3241 Fragments arranged by size: 3241 939 830 552 442 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2524 3362 4907 1132 Size: 97 1295 838 1545 2229 Fragments arranged by size: 2229 1545 1295 838 97 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 5311 PciI A'CATG_T Cuts at: 2520 4903 2520 Size: 2383 3621 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5102 PfoI T'CCnGG_A Cuts at: 2251 2251 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PmlI CAC'GTG Cuts at: 2425 3286 2425 Size: 861 5143 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2497 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 3356 830 Size: 2526 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 3135 3135 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SexAI A'CCwGG_T Cuts at: 2772 2772 Size: 6004 SfcI C'TryA_G Cuts at: 826 1045 2061 3352 4487 5168 5359 826 Size: 219 1016 1291 1135 681 191 1471 Fragments arranged by size: 1471 1291 1135 1016 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2004 2446 3276 3292 3443 513 Size: 552 939 442 830 16 151 3074 Fragments arranged by size: 3074 939 830 552 442 151 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2083 4031 4048 4201 4386 4831 325 Size: 1255 503 1948 17 153 185 445 1498 Fragments arranged by size: 1948 1498 1255 503 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 4250 5962 Size: 274 80 33 51 1443 546 1727 1712 Cuts at: 5962 96 Size: 138 Fragments arranged by size: 1727 1712 1443 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2325 2357 3272 3304 5964 5996 2325 Size: 32 915 32 2660 32 2333 Fragments arranged by size: 2660 2333 915 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2453 3290 2453 Size: 837 5167 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 2484 4133 2484 Size: 1649 4355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI