################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BseRI BsiEI BsiWI BsmI Bsp1407I BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MaeI MaeIII MfeI MflI MluI MluNI MscI MseI MspA1I MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 159 CGCG CG^CG AccIII 689 TCCGGA T^CCGGA AcyI 415 GACGCC G[GA]^CG[CT]C AlwNI 262 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 505 GTGCTC G[AT]GC[AT]^C AvaI 528 CTCGGG C^[CT]CG[GA]G AvaII 348 GGACC G^G[AT]CC BclI 641 TGATCA T^GATCA BcnI 596 CCGGG CC^[GC]GG BmyI 505 GTGCTC G[AGT]GC[ACT]^C BsaBI 642 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 415 GACGCC G[GA]^CG[CT]C BsaWI 689 TCCGGA [AT]^CCGG[AT] BseAI 689 TCCGGA T^CCGGA BsgI 15 CTGCAC GTGCAG(16/14) BsiHKAI 505 GTGCTC G[AT]GC[AT]^C BsmBI 454 GAGACG CGTCTC(1/5) BsoBI 528 CTCGGG C^[CT]CG[GA]G Bsp1286I 505 GTGCTC G[AGT]GC[ACT]^C BspDI 580 ATCGAT AT^CGAT BspEI 689 TCCGGA T^CCGGA BsrDI 618 CATTGC GCAATG(2/0) BsrFI 322 GCCGGC [GA]^CCGG[CT] BsrI 261 CCAGT ACTGG(1/-1) BssSI 451 CACGAG CACGAG(-5/-1) BstAPI 17 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 159 CGCG CG^CG BstXI 428 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 322 GCCGGC [GA]^CCGG[CT] ClaI 580 ATCGAT AT^CGAT DraII 386 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 18 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 401 CCGTGG C^C[GA][CT]GG EaeI 317 TGGCCG [CT]^GGCC[GA] EcoO109I 386 AGGCCCT [GA]G^G[ACGT]CC[CT] FbaI 641 TGATCA T^GATCA HaeII 205 GGCGCT [GA]GCGC^[CT] Hin1I 415 GACGCC G[GA]^CG[CT]C HincII 340 GTCAAC GT[CT]^[GA]AC HindII 340 GTCAAC GT[CT]^[GA]AC HinfI 256 GAATC G^A[ACGT]TC MaeII 277 ACGT A^CGT MamI 642 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 689 TCCGGA T^CCGGA MvnI 159 CGCG CG^CG NaeI 322 GCCGGC GCC^GGC NciI 596 CCGGG CC^[GC]GG NgoMIV 322 GCCGGC G^CCGGC SapI 39 GCTCTTC GCTCTTC(1/4) SfcI 391 CTACAG C^T[GA][CT]AG SmlI 508 CTCAAG C^T[CT][GA]AG TfiI 256 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 81 GATCC GGATC(4/5) 660 GATCC Asp700I 369 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 441 GAAGACCTTC BsmAI 149 GAGAC GTCTC(1/5) 454 GAGAC BsmFI 274 GGGAC GGGAC(10/14) 524 GTCCC BspHI 212 TCATGA T^CATGA 644 TCATGA BspMI 35 ACCTGC ACCTGC(4/8) 286 ACCTGC CfoI 160 GCGC GCG^C 206 GCGC DdeI 501 CTCAG C^T[ACGT]AG 664 CTGAG EcoNI 390 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 552 CCTCTTCAAGG EcoT14I 359 CCAAGG C^C[AT][AT]GG 428 CCAAGG FauI 114 CCCGC CCCGC(4/6) 157 CCCGC HgaI 415 GACGC GACGC(5/10) 456 GACGC HhaI 160 GCGC GCG^C 206 GCGC HinP1I 160 GCGC G^CGC 206 GCGC HphI 438 GGTGA GGTGA(8/7) 284 TCACC MslI 195 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 498 CACCTCAGTG RcaI 212 TCATGA T^CATGA 644 TCATGA StyI 359 CCAAGG C^C[AT][AT]GG 428 CCAAGG TaqI 581 TCGA T^CGA 653 TCGA Tsp509I 52 AATT ^AATT 517 AATT TthHB8I 581 TCGA T^CGA 653 TCGA XmnI 369 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 441 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI BbsI BbvI BpmI BpuAI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI