(Circular) (Six-base) MAPSORT of: pFN21AE5228.seq Check: 3455 from: 1 to: 5413 pFN21AE5228 5413 bp With 182 enzymes: SgfI * April 26, 2013 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2300 2300 Size: 5413 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3476 4860 5128 1632 Size: 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 2507 1166 Size: 1341 4072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2272 3156 4728 2272 Size: 884 1572 2957 Fragments arranged by size: 2957 1572 884 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2533 2738 1870 Size: 73 590 205 4545 Fragments arranged by size: 4545 590 205 73 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 4189 618 Size: 325 217 627 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 BanII G_rGCy'C Cuts at: 1391 2732 1391 Size: 1341 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2056 2453 928 Size: 971 145 12 397 3888 Fragments arranged by size: 3888 971 397 145 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 2646 1548 Size: 1098 4315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 3474 4630 1790 Size: 1684 1156 2573 Fragments arranged by size: 2573 1684 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 3144 4102 Size: 416 53 83 399 24 843 884 958 Cuts at: 4102 442 Size: 1753 Fragments arranged by size: 1753 958 884 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 2165 2464 4073 1239 Size: 858 68 299 1609 2579 Fragments arranged by size: 2579 1609 858 299 68 Bpu10I CC'TnA_GC Cuts at: 2669 2669 Size: 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 3538 4403 4701 2498 Size: 1040 865 298 3210 Fragments arranged by size: 3210 1040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 2651 5407 2651 Size: 2756 2657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 3220 3602 4237 Size: 53 83 186 936 888 799 382 635 Cuts at: 4237 275 Size: 1451 Fragments arranged by size: 1451 936 888 799 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2694 3846 4244 4518 4665 5076 2694 Size: 1152 398 274 147 411 3031 Fragments arranged by size: 3031 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2582 2612 1178 Size: 30 165 30 1179 30 3979 Fragments arranged by size: 3979 1179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5413 BseYI C'CCAG_C Cuts at: 2149 2275 4616 5112 2149 Size: 126 2341 496 2450 Fragments arranged by size: 2450 2341 496 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2005 1163 Size: 573 269 4571 Fragments arranged by size: 4571 573 269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2514 3474 3559 4630 2514 Size: 960 85 1071 3297 Fragments arranged by size: 3297 1071 960 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 BsmBI CGTCTCn'nnnn_ Cuts at: 2453 2453 Size: 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2514 2732 3474 3559 4630 1391 Size: 399 724 218 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 724 399 218 85 BspEI T'CCGG_A Cuts at: 2694 5076 2694 Size: 2382 3031 BspHI T'CATG_A Cuts at: 2217 2649 3302 5032 2217 Size: 432 653 1730 2598 Fragments arranged by size: 2598 1730 653 432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2300 2768 844 Size: 1205 251 468 3489 Fragments arranged by size: 3489 1205 468 251 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2622 3908 4082 65 Size: 1183 1374 1286 174 1396 Fragments arranged by size: 1396 1374 1286 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2327 4057 4244 1700 Size: 110 517 1730 187 2869 Fragments arranged by size: 2869 1730 517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 2456 3473 4485 2456 Size: 1017 1012 3384 Fragments arranged by size: 3384 1017 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2028 794 Size: 1234 4179 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2440 2011 Size: 429 4984 BstYI r'GATC_y Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408 Size: 771 367 385 17 1452 86 12 346 Cuts at: 5408 1972 Size: 1977 Fragments arranged by size: 1977 1452 771 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2406 513 Size: 552 939 402 3520 Fragments arranged by size: 3520 939 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2185 487 Size: 683 56 959 3715 Fragments arranged by size: 3715 959 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 2586 1554 Size: 1032 4381 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DraIII CAC_nnn'GTG Cuts at: 2028 2028 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2860 3749 Size: 54 1425 50 275 18 492 538 889 Cuts at: 3749 5378 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 538 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2564 2600 3343 1353 Size: 698 513 36 743 3423 Fragments arranged by size: 3423 743 698 513 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 4000 4374 4520 387 Size: 1064 138 510 1901 374 146 1280 Fragments arranged by size: 1901 1280 1064 510 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2165 2464 3476 4073 4860 5128 Size: 393 465 68 299 1012 597 787 268 Cuts at: 5128 1239 Size: 1524 Fragments arranged by size: 1524 1012 787 597 465 393 299 268 68 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2561 2399 Size: 162 5251 EcoO109I rG'GnC_Cy Cuts at: 2392 2392 Size: 5413 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 2214 4560 1678 Size: 536 2346 2531 Fragments arranged by size: 2531 2346 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2582 2614 4054 Size: 32 135 32 498 32 480 32 1440 Cuts at: 4054 4086 4128 4160 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1440 498 480 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 2757 3003 669 Size: 988 281 409 410 246 3079 Fragments arranged by size: 3079 988 410 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2204 2507 3371 3730 3889 518 Size: 648 843 195 303 864 359 159 2042 Fragments arranged by size: 2042 864 843 648 359 303 195 159 MspA1I CmG'CkG Cuts at: 1606 2829 3506 4654 4899 1606 Size: 1223 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 245 NaeI GCC'GGC Cuts at: 2329 2329 Size: 5413 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NgoMIV G'CCGG_C Cuts at: 2327 2327 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PfoI T'CCnGG_A Cuts at: 2256 2600 2256 Size: 344 5069 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SapI GCTCTTCn'nnn_ Cuts at: 2051 2051 Size: 5413 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2396 2761 3896 4577 4768 826 Size: 219 1351 365 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 365 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3517 4418 4680 820 Size: 197 926 570 1004 901 262 1553 Fragments arranged by size: 1553 1004 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 2433 2852 513 Size: 552 939 360 69 419 3074 Fragments arranged by size: 3074 939 552 419 360 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 3440 3457 3610 3795 4240 325 Size: 1255 501 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3659 5371 96 Size: 274 80 33 51 1443 1682 1712 138 Fragments arranged by size: 1712 1682 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 5373 5405 2252 Size: 32 3089 32 2260 Fragments arranged by size: 3089 2260 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 2378 2450 3542 2378 Size: 72 1092 4249 Fragments arranged by size: 4249 1092 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI