Restriction map for
pFN21AE5302
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI BspEI BsrI
BssSI Bst1107I BstBI BstYI BstZ17I
CelII CpoI DraI DrdI Eam1105I
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI MaeII MamI MfeI
MflI MluI MroI MseI MunI
NdeI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuII RsrII SacI SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 47 GGTACC G^GTACC
AflIII 347 ACATGT A^C[GA][CT]GT
AlwI 528 GATCC GGATC(4/5)
AocI 539 CCTCAGG CC^T[ACGT]AGG
Asp718I 47 GGTACC G^GTACC
BalI 389 TGGCCA TGG^CCA
BbeI 547 GGCGCC GGCGC^C
BcgI 192 GCAGACGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 824 CTAG C^TAG
BpmI 112 CTCCAG CTGGAG(16/14)
Bsp1407I 350 TGTACA T^GTACA
BspDI 271 ATCGAT AT^CGAT
BspLU11I 347 ACATGT A^CATGT
BsrDI 570 CATTGC GCAATG(2/0)
BsrGI 350 TGTACA T^GTACA
BssHII 210 GCGCGC G^CGCGC
BstEII 375 GGTGACC G^GT[ACGT]ACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
Bsu36I 539 CCTCAGG CC^T[ACGT]AGG
ClaI 271 ATCGAT AT^CGAT
DraIII 432 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 94 CGGCCG C^GGCCG
EarI 310 CTCTTC CTCTTC(1/4)
EclXI 94 CGGCCG C^GGCCG
Eco52I 94 CGGCCG C^GGCCG
Eco81I 539 CCTCAGG CC^T[ACGT]AGG
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
HinfI 661 GAGTC G^A[ACGT]TC
KasI 547 GGCGCC G^GCGCC
KpnI 47 GGTACC GGTAC^C
Ksp632I 310 CTCTTC CTCTTC(1/4)
KspI 380 CCGCGG CCGC^GG
MaeI 824 CTAG C^TAG
MaeIII 376 GTGAC ^GT[ACGT]AC
MboII 311 TCTTC GAAGA(8/7)
MluNI 389 TGGCCA TGG^CCA
MscI 389 TGGCCA TGG^CCA
NarI 547 GGCGCC GG^CGCC
NheI 823 GCTAGC G^CTAGC
NotI 93 GCGGCCGC GC^GGCCGC
PleI 661 GAGTC GAGTC(4/5)
PvuI 269 CGATCG CGAT^CG
SacII 380 CCGCGG CCGC^GG
SbfI 648 CCTGCAGG CCTGCA^GG
SexAI 433 ACCTGGT A^CC[AT]GGT
SfoI 547 GGCGCC GGC^GCC
SmlI 601 CTCAAG C^T[CT][GA]AG
SphI 494 GCATGC GCATG^C
Sse8387I 648 CCTGCAGG CCTGCA^GG
SspBI 350 TGTACA T^GTACA
SspI 12 AATATT AAT^ATT
Tsp45I 376 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 36 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
AlwNI 643 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
809 CAGAACCTG
ApaI 26 GGGCCC GGGCC^C
264 GGGCCC
AvaII 678 GGACC G^G[AT]CC
735 GGTCC
BglI 28 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
574 GCCATCCAGGC
BsaHI 36 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
BseRI 307 CTCCTC GAGGAG(10/8)
586 GAGGAG
BsiEI 94 CGGCCG CG[GA][CT]^CG
269 CGATCG
BsmFI 677 GGGAC GGGAC(10/14)
817 GTCCC
BspHI 524 TCATGA T^CATGA
593 TCATGA
BspMI 44 GCAGGT ACCTGC(4/8)
651 GCAGGT
BsrBI 74 CCGCTC CCGCTC(-3/-3)
169 CCGCTC
BsrFI 481 GCCGGC [GA]^CCGG[CT]
801 GCCGGC
BstAPI 248 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
431 GCACCTGGTGC
BstXI 179 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
705 CCAGGAGTTTGG
Cfr10I 481 GCCGGC [GA]^CCGG[CT]
801 GCCGGC
DdeI 540 CTCAG C^T[ACGT]AG
720 CTTAG
DraII 25 GGGGCCC [GA]G^G[ACGT]CC[CT]
60 GGGGCCT
EaeI 94 CGGCCG [CT]^GGCC[GA]
389 TGGCCA
EcoO109I 25 GGGGCCC [GA]G^G[ACGT]CC[CT]
60 GGGGCCT
EcoT14I 668 CCATGG C^C[AT][AT]GG
695 CCATGG
HaeII 547 GGCGCC [GA]GCGC^[CT]
798 AGCGCC
HgaI 37 GCGTC GACGC(5/10)
257 GCGTC
Hin1I 36 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
NaeI 481 GCCGGC GCC^GGC
801 GCCGGC
NcoI 668 CCATGG C^CATGG
695 CCATGG
NgoMIV 481 GCCGGC G^CCGGC
801 GCCGGC
NspI 347 ACATGT [GA]CATG^[CT]
494 GCATGC
PstI 130 CTGCAG CTGCA^G
649 CTGCAG
RcaI 524 TCATGA T^CATGA
593 TCATGA
StyI 668 CCATGG C^C[AT][AT]GG
695 CCATGG
TaqI 272 TCGA T^CGA
659 TCGA
TthHB8I 272 TCGA T^CGA
659 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BsgI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DsaI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TseI XmaCI XmaI