(Circular) (Six-base) MAPSORT of: pFN21AE5302.seq Check: 9181 from: 1 to: 5536 pFN21AE5302 5536 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2647 2647 Size: 5536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2052 2857 2052 Size: 805 4731 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3599 4983 5251 1632 Size: 1967 1384 268 1917 Fragments arranged by size: 1967 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 2352 4435 5412 1203 Size: 1149 2083 977 1327 Fragments arranged by size: 2083 1327 1149 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 3925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 2653 2819 3279 4851 2653 Size: 166 460 1572 3338 Fragments arranged by size: 3338 1572 460 166 ApaI G_GGCC'C Cuts at: 2035 2273 2035 Size: 238 5298 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2845 3071 3223 5332 1493 Size: 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2163 2556 2861 1870 Size: 73 91 129 393 305 4545 Fragments arranged by size: 4545 393 305 129 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2509 2542 2509 Size: 33 5503 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 2142 2489 2552 2857 Size: 325 217 627 265 90 347 63 305 Cuts at: 2857 4312 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 347 325 305 265 217 90 63 BanII G_rGCy'C Cuts at: 1391 2035 2170 2273 2471 2699 2772 2855 1391 Size: 644 135 103 198 228 73 83 4072 Fragments arranged by size: 4072 644 228 198 135 103 83 73 BbeI G_GCGC'C Cuts at: 2556 2556 Size: 5536 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2186 2220 3727 3761 1903 Size: 34 249 34 1507 34 3678 Fragments arranged by size: 3678 1507 249 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2585 4147 Size: 107 122 71 631 605 367 546 1562 Cuts at: 4147 136 Size: 1525 Fragments arranged by size: 1562 1525 631 605 546 367 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2035 2273 3597 4753 1790 Size: 245 238 1324 1156 2573 Fragments arranged by size: 2573 1324 1156 245 238 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2832 1056 Size: 1776 3760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 4196 1239 Size: 863 2094 2579 Fragments arranged by size: 2579 2094 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2591 3661 4526 4824 2591 Size: 1070 865 298 3303 Fragments arranged by size: 3303 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2553 3343 3725 Size: 53 83 186 936 509 511 790 382 Cuts at: 3725 4360 275 Size: 635 1451 Fragments arranged by size: 1451 936 790 635 511 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2606 1186 Size: 1117 303 4116 Fragments arranged by size: 4116 1117 303 BseYI C'CCAG_C Cuts at: 2062 2824 4739 5235 2062 Size: 762 1915 496 2363 Fragments arranged by size: 2363 1915 762 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2419 2801 1163 Size: 573 396 287 382 3898 Fragments arranged by size: 3898 573 396 382 287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 2277 2986 3747 4775 1540 Size: 275 186 101 175 709 761 1028 2301 Fragments arranged by size: 2301 1028 761 709 275 186 175 101 BsiHKAI G_wGCw'C Cuts at: 3597 3682 4753 3597 Size: 85 1071 4380 Fragments arranged by size: 4380 1071 85 BsmI GAATG_Cn' Cuts at: 3047 3140 3047 Size: 93 5443 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2170 2273 2471 2699 2772 2855 Size: 399 245 135 103 198 228 73 83 Cuts at: 2855 3597 3682 4753 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 245 228 198 135 103 85 83 73 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 2529 2598 3425 5155 2529 Size: 69 827 1730 2910 Fragments arranged by size: 2910 1730 827 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2040 2647 2891 844 Size: 1196 607 244 3489 Fragments arranged by size: 3489 1196 607 244 BsrBI CCG'CTC Cuts at: 1394 1823 2081 2176 3423 1394 Size: 429 258 95 1247 3507 Fragments arranged by size: 3507 1247 429 258 95 BsrDI GCAATG_nn' Cuts at: 65 1248 2574 4031 4205 65 Size: 1183 1326 1457 174 1396 Fragments arranged by size: 1457 1396 1326 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2486 2806 4180 4367 1700 Size: 110 676 320 1374 187 2869 Fragments arranged by size: 2869 1374 676 320 187 110 BsrGI T'GTAC_A Cuts at: 96 2355 96 Size: 2259 3277 BssHII G'CGCG_C Cuts at: 1925 2215 1925 Size: 290 5246 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 2259 2442 794 Size: 1465 183 3888 Fragments arranged by size: 3888 1465 183 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 2380 1184 Size: 1196 4340 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2717 2191 Size: 526 5010 BstYI r'GATC_y Cuts at: 1972 2866 3233 3618 3635 5087 5173 5185 5531 Size: 894 367 385 17 1452 86 12 346 Cuts at: 5531 1972 Size: 1977 Fragments arranged by size: 1977 1452 894 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2545 2545 Size: 5536 BtgI C'CryG_G Cuts at: 513 1065 2385 2673 2700 513 Size: 552 1320 288 27 3349 Fragments arranged by size: 3349 1320 552 288 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 3052 3846 3866 3052 Size: 794 20 4722 Fragments arranged by size: 4722 794 20 ClaI AT'CG_AT Cuts at: 1554 2277 1554 Size: 723 4813 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2557 2592 529 Size: 35 1993 35 3473 Fragments arranged by size: 3473 1993 35 35 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DraIII CAC_nnn'GTG Cuts at: 2442 2442 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 2394 2983 Size: 54 1425 50 275 18 269 295 589 Cuts at: 2983 3872 5501 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 589 295 275 269 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2099 2983 1537 Size: 275 287 884 4090 Fragments arranged by size: 4090 884 287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 3466 1353 Size: 968 1145 3423 Fragments arranged by size: 3423 1145 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2659 4123 4497 4643 387 Size: 1064 138 1070 1464 374 146 1280 Fragments arranged by size: 1464 1280 1070 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 3599 4196 4983 5251 1239 Size: 393 470 1497 597 787 268 1524 Fragments arranged by size: 1524 1497 787 597 470 393 268 EcoO109I rG'GnC_Cy Cuts at: 2031 2066 2031 Size: 35 5501 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 2556 2807 4683 1678 Size: 878 251 1876 2531 Fragments arranged by size: 2531 1876 878 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2216 2248 4177 Size: 32 135 32 580 32 32 32 1929 Cuts at: 4177 4209 4251 4283 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1929 580 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KasI G'GCGC_C Cuts at: 2552 2552 Size: 5536 KpnI G_GTAC'C Cuts at: 2056 2861 2056 Size: 805 4731 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 10 Size: 54 1425 343 564 3150 Fragments arranged by size: 3150 1425 564 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2351 2777 3494 3853 4012 518 Size: 648 1023 162 426 717 359 159 2042 Fragments arranged by size: 2042 1023 717 648 426 359 162 159 MspA1I CmG'CkG Cuts at: 1606 2098 2110 2140 2387 2952 3629 4777 5022 Size: 492 12 30 247 565 677 1148 245 Cuts at: 5022 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 565 492 247 245 30 12 NaeI GCC'GGC Cuts at: 2488 2808 2488 Size: 320 5216 NarI GG'CG_CC Cuts at: 2553 2553 Size: 5536 NcoI C'CATG_G Cuts at: 513 1065 2673 2700 513 Size: 552 1608 27 3349 Fragments arranged by size: 3349 1608 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NgoMIV G'CCGG_C Cuts at: 2486 2806 2486 Size: 320 5216 NheI G'CTAG_C Cuts at: 1052 2828 1052 Size: 1776 3760 NotI GC'GGCC_GC Cuts at: 2099 2983 2099 Size: 884 4652 NspI r_CATG'y Cuts at: 1132 1229 2356 2503 2894 4439 1132 Size: 97 1127 147 391 1545 2229 Fragments arranged by size: 2229 1545 1127 391 147 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 2352 4435 2352 Size: 2083 3453 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PspOMI G'GGCC_C Cuts at: 2031 2269 2031 Size: 238 5298 PstI C_TGCA'G Cuts at: 830 2139 2658 2888 830 Size: 1309 519 230 3478 Fragments arranged by size: 3478 1309 519 230 PvuI CG_AT'CG Cuts at: 2001 2277 2001 Size: 276 5260 SacII CC_GC'GG Cuts at: 2388 2388 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2658 2888 2658 Size: 230 5306 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SexAI A'CCwGG_T Cuts at: 2438 2438 Size: 5536 SfcI C'TryA_G Cuts at: 826 1045 2135 2654 2793 2884 4019 4700 4891 Size: 219 1090 519 139 91 1135 681 191 Cuts at: 4891 826 Size: 1471 Fragments arranged by size: 1471 1135 1090 681 519 219 191 139 91 SfoI GGC'GCC Cuts at: 2554 2554 Size: 5536 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2036 2165 2558 2863 1872 Size: 164 129 393 305 4545 Fragments arranged by size: 4545 393 305 164 129 SmlI C'TyrA_G Cuts at: 820 1017 1943 2606 3640 4541 4803 820 Size: 197 926 663 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 663 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2503 2894 1132 Size: 1371 391 3774 Fragments arranged by size: 3774 1371 391 SspI AAT'ATT Cuts at: 5 52 2019 3460 5 Size: 47 1967 1441 2081 Fragments arranged by size: 2081 1967 1441 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5536 StyI C'CwwG_G Cuts at: 513 1065 2673 2700 2975 513 Size: 552 1608 27 275 3074 Fragments arranged by size: 3074 1608 552 275 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3563 3580 3733 3918 4363 325 Size: 1255 1983 17 153 185 445 1498 Fragments arranged by size: 1983 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 2415 3782 Size: 274 80 33 51 1443 378 60 1367 Cuts at: 3782 5494 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1367 378 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2034 2163 2556 2861 1870 Size: 164 129 393 305 4545 Fragments arranged by size: 4545 393 305 164 129 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI