(Linear) (Six-base) MAPSORT of: KIEE5302.seq Check: 8087 from: 1 to: 831 KIEE5302.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 642 831 Size: 642 189 Acc65I G'GTAC_C Cuts at: 0 47 831 Size: 47 784 AflIII A'CryG_T Cuts at: 0 347 831 Size: 347 484 AleI CACnn'nnGTG Cuts at: 0 772 831 Size: 772 59 AlwNI CAG_nnn'CTG Cuts at: 0 648 814 831 Size: 648 166 17 Fragments arranged by size: 648 166 17 ApaI G_GGCC'C Cuts at: 0 30 268 831 Size: 30 238 563 Fragments arranged by size: 563 238 30 AvaI C'yCGr_G Cuts at: 0 29 158 551 831 Size: 29 129 393 280 Fragments arranged by size: 393 280 129 29 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 504 537 831 Size: 504 33 294 Fragments arranged by size: 504 294 33 BanI G'GyrC_C Cuts at: 0 47 137 484 547 831 Size: 47 90 347 63 284 Fragments arranged by size: 347 284 90 63 47 BanII G_rGCy'C Cuts at: 0 30 165 268 466 694 767 831 Size: 30 135 103 198 228 73 64 Fragments arranged by size: 228 198 135 103 73 64 30 BbeI G_GCGC'C Cuts at: 0 551 831 Size: 551 280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 181 215 831 Size: 181 34 616 Fragments arranged by size: 616 181 34 BglI GCCn_nnn'nGGC Cuts at: 0 34 580 831 Size: 34 546 251 Fragments arranged by size: 546 251 34 Bme1580I G_kGCm'C Cuts at: 0 30 268 831 Size: 30 238 563 Fragments arranged by size: 563 238 30 BmtI G_CTAG'C Cuts at: 0 827 831 Size: 827 4 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 831 Size: 97 734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 831 Size: 586 245 BsaHI Gr'CG_yC Cuts at: 0 37 548 831 Size: 37 511 283 Fragments arranged by size: 511 283 37 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 298 601 831 Size: 298 303 230 Fragments arranged by size: 303 298 230 BseYI C'CCAG_C Cuts at: 0 57 819 831 Size: 57 762 12 Fragments arranged by size: 762 57 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 414 796 831 Size: 127 287 382 35 Fragments arranged by size: 382 287 127 35 BsiEI CG_ry'CG Cuts at: 0 97 272 831 Size: 97 175 559 Fragments arranged by size: 559 175 97 Bsp1286I G_dGCh'C Cuts at: 0 30 165 268 466 694 767 831 Size: 30 135 103 198 228 73 64 Fragments arranged by size: 228 198 135 103 73 64 30 BspHI T'CATG_A Cuts at: 0 524 593 831 Size: 524 69 238 Fragments arranged by size: 524 238 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 35 642 831 Size: 35 607 189 Fragments arranged by size: 607 189 35 BsrBI CCG'CTC Cuts at: 0 76 171 831 Size: 76 95 660 Fragments arranged by size: 660 95 76 BsrDI GCAATG_nn' Cuts at: 0 569 831 Size: 569 262 BsrFI r'CCGG_y Cuts at: 0 481 801 831 Size: 481 320 30 Fragments arranged by size: 481 320 30 BsrGI T'GTAC_A Cuts at: 0 350 831 Size: 350 481 BssHII G'CGCG_C Cuts at: 0 210 831 Size: 210 621 BstAPI GCAn_nnn'nTGC Cuts at: 0 254 437 831 Size: 254 183 394 Fragments arranged by size: 394 254 183 BstEII G'GTnAC_C Cuts at: 0 375 831 Size: 375 456 BstXI CCAn_nnnn'nTGG Cuts at: 0 186 712 831 Size: 186 526 119 Fragments arranged by size: 526 186 119 Bsu36I CC'TnA_GG Cuts at: 0 540 831 Size: 540 291 BtgI C'CryG_G Cuts at: 0 380 668 695 831 Size: 380 288 27 136 Fragments arranged by size: 380 288 136 27 ClaI AT'CG_AT Cuts at: 0 272 831 Size: 272 559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 552 587 831 Size: 552 35 244 Fragments arranged by size: 552 244 35 DraIII CAC_nnn'GTG Cuts at: 0 437 831 Size: 437 394 EaeI y'GGCC_r Cuts at: 0 94 389 831 Size: 94 295 442 Fragments arranged by size: 442 295 94 EagI C'GGCC_G Cuts at: 0 94 831 Size: 94 737 EarI CTCTTCn'nnn_ Cuts at: 0 316 831 Size: 316 515 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 654 831 Size: 654 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 831 Size: 97 734 EcoO109I rG'GnC_Cy Cuts at: 0 26 61 831 Size: 26 35 770 Fragments arranged by size: 770 35 26 HaeII r_GCGC'y Cuts at: 0 551 802 831 Size: 551 251 29 Fragments arranged by size: 551 251 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 147 179 211 243 831 Size: 147 32 32 32 588 Fragments arranged by size: 588 147 32 32 32 KasI G'GCGC_C Cuts at: 0 547 831 Size: 547 284 KpnI G_GTAC'C Cuts at: 0 51 831 Size: 51 780 MscI TGG'CCA Cuts at: 0 391 831 Size: 391 440 MslI CAynn'nnrTG Cuts at: 0 184 346 772 831 Size: 184 162 426 59 Fragments arranged by size: 426 184 162 59 MspA1I CmG'CkG Cuts at: 0 93 105 135 382 831 Size: 93 12 30 247 449 Fragments arranged by size: 449 247 93 30 12 NaeI GCC'GGC Cuts at: 0 483 803 831 Size: 483 320 28 Fragments arranged by size: 483 320 28 NarI GG'CG_CC Cuts at: 0 548 831 Size: 548 283 NcoI C'CATG_G Cuts at: 0 668 695 831 Size: 668 27 136 Fragments arranged by size: 668 136 27 NgoMIV G'CCGG_C Cuts at: 0 481 801 831 Size: 481 320 30 Fragments arranged by size: 481 320 30 NheI G'CTAG_C Cuts at: 0 823 831 Size: 823 8 NotI GC'GGCC_GC Cuts at: 0 94 831 Size: 94 737 NspI r_CATG'y Cuts at: 0 351 498 831 Size: 351 147 333 Fragments arranged by size: 351 333 147 PciI A'CATG_T Cuts at: 0 347 831 Size: 347 484 PspOMI G'GGCC_C Cuts at: 0 26 264 831 Size: 26 238 567 Fragments arranged by size: 567 238 26 PstI C_TGCA'G Cuts at: 0 134 653 831 Size: 134 519 178 Fragments arranged by size: 519 178 134 PvuI CG_AT'CG Cuts at: 0 272 831 Size: 272 559 SacII CC_GC'GG Cuts at: 0 383 831 Size: 383 448 SbfI CC_TGCA'GG Cuts at: 0 653 831 Size: 653 178 SexAI A'CCwGG_T Cuts at: 0 433 831 Size: 433 398 SfcI C'TryA_G Cuts at: 0 130 649 788 831 Size: 130 519 139 43 Fragments arranged by size: 519 139 130 43 SfoI GGC'GCC Cuts at: 0 549 831 Size: 549 282 SmaI CCC'GGG Cuts at: 0 31 160 553 831 Size: 31 129 393 278 Fragments arranged by size: 393 278 129 31 SmlI C'TyrA_G Cuts at: 0 601 831 Size: 601 230 SphI G_CATG'C Cuts at: 0 498 831 Size: 498 333 SspI AAT'ATT Cuts at: 0 14 831 Size: 14 817 StyI C'CwwG_G Cuts at: 0 668 695 831 Size: 668 27 136 Fragments arranged by size: 668 136 27 TatI w'GTAC_w Cuts at: 0 350 410 831 Size: 350 60 421 Fragments arranged by size: 421 350 60 XmaI C'CCGG_G Cuts at: 0 29 158 551 831 Size: 29 129 393 280 Fragments arranged by size: 393 280 129 29 Enzymes that do cut: AarI Acc65I AflIII AleI AlwNI ApaI AvaI BaeI BanI BanII BbeI BcgI BglI Bme1580I BmtI BpmI BpuEI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI Bsu36I BtgI ClaI CspCI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PciI PspOMI PstI PvuI SacII SbfI SexAI SfcI SfoI SmaI SmlI SphI SspI StyI TatI XmaI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BssSI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI NdeI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuII RsrII SacI SalI SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI