################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI Ksp632I KspI MluI MroI MspA1I MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 802 GGTACC G^GTACC AflII 49 CTTAAG C^TTAAG AflIII 408 ACGTGT A^C[GA][CT]GT AlwNI 926 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 199 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 802 GGTACC G^GTACC BalI 1434 TGGCCA TGG^CCA BanII 332 GAGCCC G[GA]GC[CT]^C BbvI 1161 GCTGC GCAGC(8/12) BclI 1754 TGATCA T^GATCA BfrI 49 CTTAAG C^TTAAG BmyI 332 GAGCCC G[AGT]GC[ACT]^C BpmI 1775 CTCCAG CTGGAG(16/14) BsaAI 407 TACGTG [CT]AC^GT[GA] BseRI 1067 CTCCTC GAGGAG(10/8) BsiEI 813 CGGTCG CG[GA][CT]^CG BsmAI 274 GAGAC GTCTC(1/5) Bsp1286I 332 GAGCCC G[AGT]GC[ACT]^C BsrDI 1141 CATTGC GCAATG(2/0) Cac8I 176 GCCTGC GC[ACGT]^[ACGT]GC DrdI 655 GACATTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1801 CCGTGG C^C[GA][CT]GG EaeI 1434 TGGCCA [CT]^GGCC[GA] EcoRI 753 GAATTC G^AATTC EcoT22I 1793 ATGCAT ATGCA^T FauI 1335 CCCGC CCCGC(4/6) FbaI 1754 TGATCA T^GATCA Fnu4HI 1161 GCTGC GC^[ACGT]GC HaeIII 1435 GGCC GG^CC HincII 986 GTCAAC GT[CT]^[GA]AC HindII 986 GTCAAC GT[CT]^[GA]AC HindIII 708 AAGCTT A^AGCTT ItaI 1161 GCTGC GC^[ACGT]GC KpnI 802 GGTACC GGTAC^C MfeI 62 CAATTG C^AATTG MluNI 1434 TGGCCA TGG^CCA MscI 1434 TGGCCA TGG^CCA MunI 62 CAATTG C^AATTG NdeI 427 CATATG CA^TATG NsiI 1793 ATGCAT ATGCA^T ScaI 1659 AGTACT AGT^ACT SfcI 723 CTATAG C^T[GA][CT]AG SmlI 49 CTTAAG C^T[CT][GA]AG TaqI 1399 TCGA T^CGA TseI 1161 GCTGC G^C[AT]GC TthHB8I 1399 TCGA T^CGA XcmI 568 CCATTTCCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 199 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 664 GTCTAC GT^[AC][GT]AC 1352 GTCTAC AciI 1080 CCGC CCGC(-3/-1) 1336 CCGC BanI 556 GGCACC G^G[CT][GA]CC 802 GGTACC BbsI 1115 GAAGAC GAAGAC(2/6) 1441 GAAGAC BfaI 1520 CTAG C^TAG 1663 CTAG BglII 691 AGATCT A^GATCT 1429 AGATCT BpuAI 1115 GAAGAC GAAGAC(2/6) 1441 GAAGAC BsaJI 1550 CCCTGG C^C[ACGT][ACGT]GG 1801 CCGTGG BsmI 1810 GAATGC GAATGC(1/-1) 903 GCATTC BsrI 1596 ACTGG ACTGG(1/-1) 806 CCAGT DraIII 1040 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 1376 CACTCAGTG MaeI 1520 CTAG C^TAG 1663 CTAG MaeII 313 ACGT A^CGT 408 ACGT MwoI 350 GCACTTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 941 GCTTTCCAAGC PflMI 387 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 946 CCAAGCTCTGG PleI 276 GACTC GAGTC(4/5) 584 GAGTC Van91I 387 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 946 CCAAGCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BsaBI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII FokI HinfI HphI MaeIII MamI MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I TspRI XhoII