(Circular) (Six-base) MAPSORT of: pFN21AE4544.seq Check: 2142 from: 1 to: 6607 pFN21AE4544 6607 bp With 182 enzymes: SgfI * March 28, 2012 14:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2670 3358 3950 1937 Size: 733 688 592 4594 Fragments arranged by size: 4594 733 688 592 Acc65I G'GTAC_C Cuts at: 2807 3928 2807 Size: 1121 5486 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2723 3725 4670 6054 6322 1632 Size: 644 447 1002 945 1384 268 1917 Fragments arranged by size: 1917 1384 1002 945 644 447 268 AflII C'TTAA_G Cuts at: 820 1017 2054 820 Size: 197 1037 5373 Fragments arranged by size: 5373 1037 197 AflIII A'CryG_T Cuts at: 1203 2413 5506 6483 1203 Size: 1210 3093 977 1327 Fragments arranged by size: 3093 1327 1210 977 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6607 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 2223 Size: 32 6575 AlwNI CAG_nnn'CTG Cuts at: 2936 4350 5922 2936 Size: 1414 1572 3621 Fragments arranged by size: 3621 1572 1414 ApaLI G'TGCA_C Cuts at: 4664 5820 4664 Size: 1156 5451 ApoI r'AATT_y Cuts at: 1493 2758 2890 3390 3550 3695 3861 3916 4142 Size: 1265 132 500 160 145 166 55 226 Cuts at: 4142 4294 6403 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1265 500 226 166 160 152 145 132 55 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 3932 1870 Size: 73 1989 4545 Fragments arranged by size: 4545 1989 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2055 2088 2055 Size: 33 6574 BamHI G'GATC_C Cuts at: 3937 4304 3937 Size: 367 6240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 2807 3928 5383 618 Size: 325 217 627 774 246 1121 1455 1842 Fragments arranged by size: 1842 1455 1121 774 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 2341 3926 1391 Size: 950 1585 4072 Fragments arranged by size: 4072 1585 950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3127 3453 928 Size: 971 1228 326 4082 Fragments arranged by size: 4082 1228 971 326 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4798 4832 1903 Size: 34 2861 34 3678 Fragments arranged by size: 3678 2861 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4499 5709 4499 Size: 1210 5397 BclI T'GATC_A Cuts at: 1548 3759 1548 Size: 2211 4396 BfrBI ATG'CAT Cuts at: 3800 3800 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2696 3434 6602 2696 Size: 738 3168 2701 Fragments arranged by size: 3168 2701 738 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 4668 5824 1790 Size: 2878 1156 2573 Fragments arranged by size: 2878 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3611 4338 5296 Size: 416 53 83 399 24 2194 727 958 Cuts at: 5296 442 Size: 1753 Fragments arranged by size: 2194 1753 958 727 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3398 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3765 5267 1239 Size: 2526 1502 2579 Fragments arranged by size: 2579 2526 1502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4732 5597 5895 4732 Size: 865 298 5444 Fragments arranged by size: 5444 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5270 882 Size: 617 3771 2219 Fragments arranged by size: 3771 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2414 4398 493 Size: 711 1210 1984 2702 Fragments arranged by size: 2702 1984 1210 711 BsaBI GATnn'nnATC Cuts at: 2859 2965 3801 6601 2859 Size: 106 836 2800 2865 Fragments arranged by size: 2865 2800 836 106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4414 4796 5431 275 Size: 53 83 186 936 2881 382 635 1451 Fragments arranged by size: 2881 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5040 5438 5712 5859 6270 5040 Size: 398 274 147 411 5377 Fragments arranged by size: 5377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 1178 Size: 30 165 30 820 30 5532 Fragments arranged by size: 5532 820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3063 1186 Size: 1877 4730 BseYI C'CCAG_C Cuts at: 5810 6306 5810 Size: 496 6111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6034 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2821 4057 4818 5846 1540 Size: 275 186 820 1236 761 1028 2301 Fragments arranged by size: 2301 1236 1028 820 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4668 4753 5824 4668 Size: 85 1071 5451 Fragments arranged by size: 5451 1071 85 BsmI GAATG_Cn' Cuts at: 2908 3821 4118 4211 2908 Size: 913 297 93 5304 Fragments arranged by size: 5304 913 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 3926 4668 4753 5824 1391 Size: 399 551 1585 742 85 1071 2174 Fragments arranged by size: 2174 1585 1071 742 551 399 85 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 4496 6226 4496 Size: 1730 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3962 844 Size: 3118 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4494 1394 Size: 429 2671 3507 Fragments arranged by size: 3507 2671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3145 5102 5276 65 Size: 1183 1897 1957 174 1396 Fragments arranged by size: 1957 1897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5251 5438 1700 Size: 110 3441 187 2869 Fragments arranged by size: 3441 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 4667 5679 4667 Size: 1012 5595 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6607 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2696 3434 3533 3937 4304 4689 4706 6158 Size: 724 738 99 404 367 385 17 1452 Cuts at: 6158 6244 6256 6602 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 738 724 404 385 367 346 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3806 513 Size: 552 2741 3314 Fragments arranged by size: 3314 2741 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5868 Fragments arranged by size: 5868 683 56 BtsI GCAGTG_nn' Cuts at: 4123 4917 4937 4123 Size: 794 20 5793 Fragments arranged by size: 5793 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DraIII CAC_nnn'GTG Cuts at: 3050 3386 3050 Size: 336 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2666 5614 809 Size: 507 1350 2948 1802 Fragments arranged by size: 2948 1802 1350 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4054 4943 Size: 54 1425 50 275 18 1609 615 889 Cuts at: 4943 6572 8 Size: 1629 43 Fragments arranged by size: 1629 1609 1425 889 615 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4054 1537 Size: 275 2242 4090 Fragments arranged by size: 4090 2242 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4537 1353 Size: 3184 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5194 5568 5714 387 Size: 1064 138 3605 374 146 1280 Fragments arranged by size: 3605 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2723 3725 3765 4670 5267 6054 Size: 393 644 447 1002 40 905 597 787 Cuts at: 6054 6322 1239 Size: 268 1524 Fragments arranged by size: 1524 1002 905 787 644 597 447 393 268 40 EcoRI G'AATT_C Cuts at: 2758 3916 4294 2758 Size: 1158 378 5071 Fragments arranged by size: 5071 1158 378 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 5754 1678 Size: 4076 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2328 2360 2519 Size: 32 135 32 548 32 176 32 159 Cuts at: 2519 2551 2867 2887 2899 2919 3554 3586 5248 Size: 32 316 20 12 20 635 32 1662 Cuts at: 5248 5280 5322 5354 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1662 635 548 316 176 159 135 42 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2993 3951 4197 669 Size: 988 281 1055 958 246 3079 Fragments arranged by size: 3079 1055 988 958 281 246 HindIII A'AGCT_T Cuts at: 748 2713 748 Size: 1965 4642 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KpnI G_GTAC'C Cuts at: 2811 3932 2811 Size: 1121 5486 MfeI C'AATT_G Cuts at: 2067 4206 2067 Size: 2139 4468 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2689 3792 5721 5905 1305 Size: 1384 1103 1929 184 2007 Fragments arranged by size: 2007 1929 1384 1103 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3441 10 Size: 54 1425 343 1609 3176 Fragments arranged by size: 3176 1609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2685 2799 2913 3046 3504 3767 3805 Size: 648 1519 114 114 133 458 263 38 Cuts at: 3805 4565 4924 5083 518 Size: 760 359 159 2042 Fragments arranged by size: 2042 1519 760 648 458 359 263 159 133 114 114 38 MspA1I CmG'CkG Cuts at: 1606 4023 4700 5848 6093 1606 Size: 2417 677 1148 245 2120 Fragments arranged by size: 2417 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6055 NdeI CA'TA_TG Cuts at: 387 2433 387 Size: 2046 4561 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NsiI A_TGCA'T Cuts at: 3802 3802 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 3965 5510 1132 Size: 97 2736 1545 2229 Fragments arranged by size: 2736 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 2957 1108 Size: 1290 559 4758 Fragments arranged by size: 4758 1290 559 PfoI T'CCnGG_A Cuts at: 3781 3781 Size: 6607 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2223 2255 4676 4708 6189 6221 2223 Size: 32 2421 32 1481 32 2609 Fragments arranged by size: 2609 2421 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 2223 4177 2223 Size: 1954 4653 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3505 3537 3505 Size: 32 6575 PstI C_TGCA'G Cuts at: 830 3959 830 Size: 3129 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 3666 4855 6567 3666 Size: 1189 1712 3706 Fragments arranged by size: 3706 1712 1189 SfcI C'TryA_G Cuts at: 826 1045 2728 3955 5090 5771 5962 826 Size: 219 1683 1227 1135 681 191 1471 Fragments arranged by size: 1683 1471 1227 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 4711 5612 5874 820 Size: 197 926 111 2657 901 262 1553 Fragments arranged by size: 2657 1553 926 901 262 197 111 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6607 StyI C'CwwG_G Cuts at: 513 1065 4046 513 Size: 552 2981 3074 Fragments arranged by size: 3074 2981 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4634 4651 4804 4989 5434 325 Size: 1255 3054 17 153 185 445 1498 Fragments arranged by size: 3054 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3664 4853 6565 Size: 274 80 33 51 1443 1687 1189 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 1712 1687 1443 1189 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6567 6599 6567 Size: 32 6575 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2580 2580 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 2208 4736 2208 Size: 2528 4079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI