Restriction map for
pFN21AE4544
(Linear) (Six-base) MAPSORT of: KIEE4544.seq Check: 189 from: 1 to: 1902
KIEE4544.orf
With 182 enzymes: SgfI *
March 22, 2012 18:19 ..
AccI GT'mk_AC
Cuts at: 0 665 1353 1902
Size: 665 688 549
Fragments arranged by size:
688 665 549
Acc65I G'GTAC_C
Cuts at: 0 802 1902
Size: 802 1100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 718 1720 1902
Size: 271 447 1002 182
Fragments arranged by size:
1002 447 271 182
AflII C'TTAA_G
Cuts at: 0 49 1902
Size: 49 1853
AflIII A'CryG_T
Cuts at: 0 408 1902
Size: 408 1494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 1902
Size: 218 32 1652
Fragments arranged by size:
1652 218 32
AlwNI CAG_nnn'CTG
Cuts at: 0 931 1902
Size: 931 971
ApoI r'AATT_y
Cuts at: 0 753 885 1385 1545 1690 1856 1902
Size: 753 132 500 160 145 166 46
Fragments arranged by size:
753 500 166 160 145 132 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 50 83 1902
Size: 50 33 1819
Fragments arranged by size:
1819 50 33
BanI G'GyrC_C
Cuts at: 0 556 802 1902
Size: 556 246 1100
Fragments arranged by size:
1100 556 246
BanII G_rGCy'C
Cuts at: 0 336 1902
Size: 336 1566
BbsI GAAGACnn'nnnn_
Cuts at: 0 1122 1448 1902
Size: 1122 326 454
Fragments arranged by size:
1122 454 326
BclI T'GATC_A
Cuts at: 0 1754 1902
Size: 1754 148
BfrBI ATG'CAT
Cuts at: 0 1795 1902
Size: 1795 107
BglII A'GATC_T
Cuts at: 0 691 1429 1902
Size: 691 738 473
Fragments arranged by size:
738 691 473
BmrI ACTGGGnnnn_n'
Cuts at: 0 1606 1902
Size: 1606 296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1902
Size: 1393 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1760 1902
Size: 1760 142
BsaAI yAC'GTr
Cuts at: 0 409 1902
Size: 409 1493
BsaBI GATnn'nnATC
Cuts at: 0 854 960 1796 1902
Size: 854 106 836 106
Fragments arranged by size:
854 836 106 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 218 248 1902
Size: 218 30 1654
Fragments arranged by size:
1654 218 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1058 1902
Size: 1058 844
BsiEI CG_ry'CG
Cuts at: 0 816 1902
Size: 816 1086
BsmI GAATG_Cn'
Cuts at: 0 903 1816 1902
Size: 903 913 86
Fragments arranged by size:
913 903 86
Bsp1286I G_dGCh'C
Cuts at: 0 336 1902
Size: 336 1566
BsrDI GCAATG_nn'
Cuts at: 0 1140 1902
Size: 1140 762
BstYI r'GATC_y
Cuts at: 0 691 1429 1528 1902
Size: 691 738 99 374
Fragments arranged by size:
738 691 374 99
BtgI C'CryG_G
Cuts at: 0 1801 1902
Size: 1801 101
DraIII CAC_nnn'GTG
Cuts at: 0 1045 1381 1902
Size: 1045 336 521
Fragments arranged by size:
1045 521 336
DrdI GACnn_nn'nnGTC
Cuts at: 0 661 1902
Size: 661 1241
EaeI y'GGCC_r
Cuts at: 0 1434 1902
Size: 1434 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 718 1720 1760 1902
Size: 271 447 1002 40 142
Fragments arranged by size:
1002 447 271 142 40
EcoRI G'AATT_C
Cuts at: 0 753 1902
Size: 753 1149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 323 355 514 546 862 882
Size: 115 32 176 32 159 32 316 20
Cuts at: 882 894 914 1549 1581 1902
Size: 12 20 635 32 321
Fragments arranged by size:
635 321 316 176 159 115 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 988 1902
Size: 988 914
HindIII A'AGCT_T
Cuts at: 0 708 1902
Size: 708 1194
KpnI G_GTAC'C
Cuts at: 0 806 1902
Size: 806 1096
MfeI C'AATT_G
Cuts at: 0 62 1902
Size: 62 1840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 1787 1902
Size: 684 1103 115
Fragments arranged by size:
1103 684 115
MscI TGG'CCA
Cuts at: 0 1436 1902
Size: 1436 466
MslI CAynn'nnrTG
Cuts at: 0 680 794 908 1041 1499 1762 1800 1902
Size: 680 114 114 133 458 263 38 102
Fragments arranged by size:
680 458 263 133 114 114 102 38
NdeI CA'TA_TG
Cuts at: 0 428 1902
Size: 428 1474
NsiI A_TGCA'T
Cuts at: 0 1797 1902
Size: 1797 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 952 1902
Size: 393 559 950
Fragments arranged by size:
950 559 393
PfoI T'CCnGG_A
Cuts at: 0 1776 1902
Size: 1776 126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 1902
Size: 218 32 1652
Fragments arranged by size:
1652 218 32
PsiI TTA'TAA
Cuts at: 0 218 1902
Size: 218 1684
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1500 1532 1902
Size: 1500 32 370
Fragments arranged by size:
1500 370 32
ScaI AGT'ACT
Cuts at: 0 1661 1902
Size: 1661 241
SfcI C'TryA_G
Cuts at: 0 723 1902
Size: 723 1179
SmlI C'TyrA_G
Cuts at: 0 49 1902
Size: 49 1853
TatI w'GTAC_w
Cuts at: 0 1659 1902
Size: 1659 243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 575 1902
Size: 575 1327
XmnI GAAnn'nnTTC
Cuts at: 0 203 1902
Size: 203 1699
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AloI AlwNI ApoI
BaeI BanI BanII BbsI BclI BfrBI BglII BmrI
BplI BpmI BsaAI BsaBI BsaXI BseRI BsiEI BsmI
Bsp1286I BsrDI BstYI BtgI DraIII DrdI EaeI Eco57MI
EcoRI Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI NdeI NsiI PflMI PfoI PpiI PsiI PsrI
ScaI SfcI SmlI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmtI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI MluI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI