################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BsaAI BsaBI BsaHI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoT22I FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 1411 CGCG CG^CG AlwNI 1621 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 862 CCTGAGG CC^T[ACGT]AGG ApaI 314 GGGCCC GGGCC^C AvrII 1065 CCTAGG C^CTAGG BalI 2039 TGGCCA TGG^CCA BamHI 432 GGATCC G^GATCC BglII 1667 AGATCT A^GATCT BlnI 1065 CCTAGG C^CTAGG BsaI 424 GAGACC GGTCTC(1/5) BsaWI 1106 TCCGGT [AT]^CCGG[AT] BsiEI 1709 CGGTCG CG[GA][CT]^CG BsmBI 1962 GAGACG CGTCTC(1/5) BsrDI 1203 CATTGC GCAATG(2/0) BsrFI 32 GCCGGC [GA]^CCGG[CT] BssSI 1308 CTCGTG CACGAG(-5/-1) BstAPI 468 GCACGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 904 GGTGACC G^GT[ACGT]ACC BstPI 904 GGTGACC G^GT[ACGT]ACC BstUI 1411 CGCG CG^CG Bsu36I 862 CCTGAGG CC^T[ACGT]AGG Cfr10I 32 GCCGGC [GA]^CCGG[CT] DraIII 469 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1162 GACTGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 862 CCTGAGG CC^T[ACGT]AGG EcoO65I 904 GGTGACC G^GT[ACGT]ACC EcoRI 1777 GAATTC G^AATTC HgaI 181 GACGC GACGC(5/10) HindIII 2235 AAGCTT A^AGCTT MaeII 151 ACGT A^CGT MluNI 2039 TGGCCA TGG^CCA MscI 2039 TGGCCA TGG^CCA MspA1I 540 CAGCTG C[AC]G^C[GT]G MvnI 1411 CGCG CG^CG NaeI 32 GCCGGC GCC^GGC NdeI 1657 CATATG CA^TATG NgoMIV 32 GCCGGC G^CCGGC PaeR7I 1738 CTCGAG C^TCGAG PflMI 647 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 540 CAGCTG CAG^CTG SacI 2143 GAGCTC GAGCT^C SapI 2178 GCTCTTC GCTCTTC(1/4) SmaI 716 CCCGGG CCC^GGG Van91I 647 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1738 CTCGAG C^TCGAG XmaCI 716 CCCGGG C^CCGGG XmaI 716 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 802 GTCTAC GT^[AC][GT]AC 1924 GTAGAC AcsI 256 AAATTT [GA]^AATT[CT] 1777 GAATTC AfaI 1176 GTAC GT^AC 1647 GTAC AlwI 433 GATCC GGATC(4/5) 432 GGATC Aor51HI 941 AGCGCT AGC^GCT 1353 AGCGCT ApoI 256 AAATTT [GA]^AATT[CT] 1777 GAATTC Asp700I 457 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2175 GAAGCTCTTC AspHI 1041 GTGCTC G[AT]GC[AT]^C 2143 GAGCTC BanI 858 GGTGCC G^G[CT][GA]CC 1057 GGCACC BanII 314 GGGCCC G[GA]GC[CT]^C 2143 GAGCTC BbsI 1491 GAAGAC GAAGAC(2/6) 1888 GAAGAC BfaI 830 CTAG C^TAG 1066 CTAG BlpI 1836 GCTCAGC GC^T[ACGT]AGC 2145 GCTCAGC Bpu1102I 1836 GCTCAGC GC^T[ACGT]AGC 2145 GCTCAGC BpuAI 1491 GAAGAC GAAGAC(2/6) 1888 GAAGAC BseRI 1975 GAGGAG GAGGAG(10/8) 1996 GAGGAG BsiHKAI 1041 GTGCTC G[AT]GC[AT]^C 2143 GAGCTC BstF5I 1860 CATCC GGATG(2/0) 381 GGATG BstYI 432 GGATCC [GA]^GATC[CT] 1667 AGATCT CelII 1836 GCTCAGC GC^T[ACGT]AGC 2145 GCTCAGC Eco47III 941 AGCGCT AGC^GCT 1353 AGCGCT EcoRV 223 GATATC GAT^ATC 1402 GATATC FokI 1860 CATCC GGATG(9/13) 381 GGATG HaeII 941 AGCGCT [GA]GCGC^[CT] 1353 AGCGCT MaeI 830 CTAG C^TAG 1066 CTAG MflI 432 GGATCC [GA]^GATC[CT] 1667 AGATCT NcoI 1049 CCATGG C^CATGG 1343 CCATGG PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT] 205 AGGTCCT RsaI 1176 GTAC GT^AC 1647 GTAC XcmI 1334 CCAGCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2120 CCACACATGACATGG XhoII 432 GGATCC [GA]^GATC[CT] 1667 AGATCT XmnI 457 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2175 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AluI AvaI AvaII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I