(Circular) (Six-base) MAPSORT of: pFN21AE5701.seq Check: 6213 from: 1 to: 6949 pFN21AE5701 6949 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 278 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2808 3930 4292 1937 Size: 871 1122 362 4594 Fragments arranged by size: 4594 1122 871 362 Acc65I G'GTAC_C Cuts at: 4270 4270 Size: 6949 AclI AA'CG_TT Cuts at: 1563 5076 5449 1563 Size: 3513 373 3063 Fragments arranged by size: 3513 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2385 2423 3689 3752 4196 5012 6396 6664 Size: 753 38 1266 63 444 816 1384 268 Cuts at: 6664 1632 Size: 1917 Fragments arranged by size: 1917 1384 1266 816 753 444 268 63 38 AfeI AGC'GCT Cuts at: 2948 3360 2948 Size: 412 6537 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6752 AflIII A'CryG_T Cuts at: 1203 5848 6825 1203 Size: 4645 977 1327 Fragments arranged by size: 4645 1327 977 AgeI A'CCGG_T Cuts at: 5780 5780 Size: 6949 AhdI GACnn_n'nnGTC Cuts at: 5678 5678 Size: 6949 AleI CACnn'nnGTG Cuts at: 1166 3096 3917 1166 Size: 1930 821 4198 Fragments arranged by size: 4198 1930 821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6949 AlwNI CAG_nnn'CTG Cuts at: 3631 4692 6264 3631 Size: 1061 1572 4316 Fragments arranged by size: 4316 1572 1061 ApaI G_GGCC'C Cuts at: 2323 2323 Size: 6949 ApaLI G'TGCA_C Cuts at: 5006 6162 5006 Size: 1156 5793 ApoI r'AATT_y Cuts at: 1493 2261 3782 4258 4484 4636 6745 1493 Size: 768 1521 476 226 152 2109 1697 Fragments arranged by size: 2109 1697 1521 768 476 226 152 AseI AT'TA_AT Cuts at: 160 1032 5503 160 Size: 872 4471 1606 Fragments arranged by size: 4471 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 AvaI C'yCGr_G Cuts at: 1870 1943 2721 3743 3810 3872 4274 1870 Size: 73 778 1022 67 62 402 4545 Fragments arranged by size: 4545 1022 778 402 73 67 62 AvrII C'CTAG_G Cuts at: 3070 3070 Size: 6949 BamHI G'GATC_C Cuts at: 2437 4279 4646 2437 Size: 1842 367 4740 Fragments arranged by size: 4740 1842 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2863 3062 4270 5725 618 Size: 325 217 627 1076 199 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 1076 627 325 217 199 BanII G_rGCy'C Cuts at: 1391 2323 4152 4268 1391 Size: 932 1829 116 4072 Fragments arranged by size: 4072 1829 932 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3503 3900 928 Size: 971 1604 397 3977 Fragments arranged by size: 3977 1604 971 397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5140 5174 1903 Size: 34 3203 34 3678 Fragments arranged by size: 3678 3203 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4841 6051 4841 Size: 1210 5739 BclI T'GATC_A Cuts at: 1548 1548 Size: 6949 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5560 136 Size: 107 122 71 631 605 3888 1525 Fragments arranged by size: 3888 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3672 6944 3672 Size: 3272 3677 BlpI GC'TnA_GC Cuts at: 3842 4151 4366 3842 Size: 309 215 6425 Fragments arranged by size: 6425 309 215 Bme1580I G_kGCm'C Cuts at: 1790 2205 2323 3011 5010 6166 1790 Size: 415 118 688 1999 1156 2573 Fragments arranged by size: 2573 1999 1156 688 415 118 BmgBI CAC'GTC Cuts at: 1318 2157 1318 Size: 839 6110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2047 4059 4680 Size: 416 53 83 399 24 630 2012 621 Cuts at: 4680 5638 442 Size: 958 1753 Fragments arranged by size: 2012 1753 958 630 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6949 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2965 3715 2863 Size: 102 750 6097 Fragments arranged by size: 6097 750 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2691 3322 3656 5609 1239 Size: 1452 631 334 1953 2579 Fragments arranged by size: 2579 1953 1452 631 334 Bpu10I CC'TnA_GC Cuts at: 2915 4194 2915 Size: 1279 5670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2121 2254 2522 5074 5939 6237 2121 Size: 133 268 2552 865 298 2833 Fragments arranged by size: 2833 2552 865 298 268 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2423 5612 882 Size: 617 924 3189 2219 Fragments arranged by size: 3189 2219 924 617 BsaAI yAC'GTr Cuts at: 493 1204 4740 493 Size: 711 3536 2702 Fragments arranged by size: 3536 2702 711 BsaBI GATnn'nnATC Cuts at: 6943 6943 Size: 6949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4756 5138 5773 275 Size: 53 83 186 936 3223 382 635 1451 Fragments arranged by size: 3223 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3111 5382 5780 6054 6201 6612 3111 Size: 2271 398 274 147 411 3448 Fragments arranged by size: 3448 2271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2523 2553 4082 4112 1178 Size: 30 165 30 1120 30 1529 30 4015 Fragments arranged by size: 4015 1529 1120 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3995 4016 1186 Size: 2809 21 4119 Fragments arranged by size: 4119 2809 21 BseYI C'CCAG_C Cuts at: 2069 2076 3171 6152 6648 2069 Size: 7 1095 2981 496 2370 Fragments arranged by size: 2981 2370 1095 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2501 2534 3173 1163 Size: 573 330 435 33 639 4939 Fragments arranged by size: 4939 639 573 435 330 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3717 4399 5160 6188 1540 Size: 275 186 1716 682 761 1028 2301 Fragments arranged by size: 2301 1716 1028 761 682 275 186 BsiHKAI G_wGCw'C Cuts at: 3050 4152 5010 5095 6166 3050 Size: 1102 858 85 1071 3833 Fragments arranged by size: 3833 1102 1071 858 85 BsmI GAATG_Cn' Cuts at: 4460 4553 4460 Size: 93 6856 BsmBI CGTCTCn'nnnn_ Cuts at: 3961 3961 Size: 6949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2323 3011 3050 4152 4268 5010 Size: 399 415 118 688 39 1102 116 742 Cuts at: 5010 5095 6166 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1102 1071 742 688 415 399 118 116 85 39 BspEI T'CCGG_A Cuts at: 6612 6612 Size: 6949 BspHI T'CATG_A Cuts at: 4838 6568 4838 Size: 1730 5219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4304 844 Size: 3460 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4836 1394 Size: 429 3013 3507 Fragments arranged by size: 3507 3013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3207 5444 5618 65 Size: 1183 1959 2237 174 1396 Fragments arranged by size: 2237 1959 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 5593 5780 1700 Size: 110 227 3556 187 2869 Fragments arranged by size: 3556 2869 227 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6949 BssSI C'ACGA_G Cuts at: 3313 5009 6021 3313 Size: 1696 1012 4241 Fragments arranged by size: 4241 1696 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2479 794 Size: 1685 5264 BstBI TT'CG_AA Cuts at: 5753 5753 Size: 6949 BstEII G'GTnAC_C Cuts at: 1184 2909 1184 Size: 1725 5224 BstYI r'GATC_y Cuts at: 1972 2437 3672 4279 4646 5031 5048 6500 6586 Size: 465 1235 607 367 385 17 1452 86 Cuts at: 6586 6598 6944 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1235 607 465 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2868 2868 Size: 6949 BtgI C'CryG_G Cuts at: 513 1065 2004 2433 3054 3348 513 Size: 552 939 429 621 294 4114 Fragments arranged by size: 4114 939 621 552 429 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3628 3861 487 Size: 683 56 2402 233 3575 Fragments arranged by size: 3575 2402 683 233 56 BtsI GCAGTG_nn' Cuts at: 2484 4465 5259 5279 2484 Size: 1981 794 20 4154 Fragments arranged by size: 4154 1981 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6949 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6914 DraI TTT'AAA Cuts at: 4253 4253 Size: 6949 DraIII CAC_nnn'GTG Cuts at: 2479 2479 Size: 6949 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3173 5956 809 Size: 507 1857 2783 1802 Fragments arranged by size: 2783 1857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3002 3051 4044 Size: 54 1425 50 275 18 1172 49 993 Cuts at: 4044 4396 5285 6914 8 Size: 352 889 1629 43 Fragments arranged by size: 1629 1425 1172 993 889 352 275 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4396 1537 Size: 275 2584 4090 Fragments arranged by size: 4090 2584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2233 2351 3546 3608 3684 3972 3980 4190 Size: 880 118 1195 62 76 288 8 210 Cuts at: 4190 4879 1353 Size: 689 3423 Fragments arranged by size: 3423 1195 880 689 288 210 118 76 62 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5536 5910 6056 387 Size: 1064 138 3947 374 146 1280 Fragments arranged by size: 3947 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4150 4150 Size: 6949 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2385 2423 2691 3322 3656 3689 3752 Size: 393 753 38 268 631 334 33 63 Cuts at: 3752 4196 5012 5609 6396 6664 1239 Size: 444 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 753 631 597 444 393 334 268 268 63 38 33 EcoO109I rG'GnC_Cy Cuts at: 2073 2211 2319 3722 2073 Size: 138 108 1403 5300 Fragments arranged by size: 5300 1403 138 108 EcoRI G'AATT_C Cuts at: 3782 4258 4636 3782 Size: 476 378 6095 Fragments arranged by size: 6095 476 378 EcoRV GAT'ATC Cuts at: 2230 3409 2230 Size: 1179 5770 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 3428 4201 2272 Size: 1156 773 5020 Fragments arranged by size: 5020 1156 773 FspI TGC'GCA Cuts at: 5455 5455 Size: 6949 HaeII r_GCGC'y Cuts at: 1678 2950 3362 6096 1678 Size: 1272 412 2734 2531 Fragments arranged by size: 2734 2531 1272 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 2523 2555 2570 Size: 32 135 32 850 32 69 32 15 Cuts at: 2570 2602 3699 3731 4082 4114 5590 5622 5664 Size: 32 1097 32 351 32 1476 32 42 Cuts at: 5664 5696 1373 Size: 32 2626 Fragments arranged by size: 2626 1476 1097 850 351 135 69 42 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2542 3427 3532 4293 4539 669 Size: 988 281 604 885 105 761 246 3079 Fragments arranged by size: 3079 988 885 761 604 281 246 105 HindIII A'AGCT_T Cuts at: 748 4240 748 Size: 3492 3457 HpaI GTT'AAC Cuts at: 4539 4539 Size: 6949 KpnI G_GTAC'C Cuts at: 4274 4274 Size: 6949 MfeI C'AATT_G Cuts at: 4548 4548 Size: 6949 MluI A'CGCG_T Cuts at: 6825 6825 Size: 6949 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2857 3126 3697 6063 6247 1305 Size: 1552 269 571 2366 184 2007 Fragments arranged by size: 2366 2007 1552 571 269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4046 10 Size: 54 1425 343 2214 2913 Fragments arranged by size: 2913 2214 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 3096 3129 3545 3662 3755 3917 Size: 648 1684 246 33 416 117 93 162 Cuts at: 3917 4907 5266 5425 518 Size: 990 359 159 2042 Fragments arranged by size: 2042 1684 990 648 416 359 246 162 159 117 93 33 MspA1I CmG'CkG Cuts at: 1606 2547 4365 5042 6190 6435 1606 Size: 941 1818 677 1148 245 2120 Fragments arranged by size: 2120 1818 1148 941 677 245 NaeI GCC'GGC Cuts at: 2039 2039 Size: 6949 NcoI C'CATG_G Cuts at: 513 1065 2004 3054 3348 513 Size: 552 939 1050 294 4114 Fragments arranged by size: 4114 1050 939 552 294 NdeI CA'TA_TG Cuts at: 387 3663 387 Size: 3276 3673 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 6949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6949 NotI GC'GGCC_GC Cuts at: 4396 4396 Size: 6949 NspI r_CATG'y Cuts at: 1132 1229 4307 5852 1132 Size: 97 3078 1545 2229 Fragments arranged by size: 3078 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3703 1348 Size: 2355 4594 PciI A'CATG_T Cuts at: 5848 5848 Size: 6949 PflMI CCAn_nnn'nTGG Cuts at: 1108 2658 1108 Size: 1550 5399 PmeI GTTT'AAAC Cuts at: 4253 4253 Size: 6949 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5018 5050 6531 6563 5018 Size: 32 1481 32 5404 Fragments arranged by size: 5404 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 2211 2073 Size: 138 6811 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6949 PsiI TTA'TAA Cuts at: 4519 4519 Size: 6949 PspOMI G'GGCC_C Cuts at: 2319 2319 Size: 6949 PstI C_TGCA'G Cuts at: 830 2929 3840 4224 4301 830 Size: 2099 911 384 77 3478 Fragments arranged by size: 3478 2099 911 384 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6949 PvuII CAG'CTG Cuts at: 2547 2547 Size: 6949 SacI G_AGCT'C Cuts at: 4152 4152 Size: 6949 SalI G'TCGA_C Cuts at: 1936 4291 1936 Size: 2355 4594 SanDI GG'GwC_CC Cuts at: 2073 2073 Size: 6949 SapI GCTCTTCn'nnn_ Cuts at: 4190 4190 Size: 6949 SbfI CC_TGCA'GG Cuts at: 4301 4301 Size: 6949 ScaI AGT'ACT Cuts at: 5197 6909 5197 Size: 1712 5237 SfcI C'TryA_G Cuts at: 826 1045 2925 3836 4220 4297 5432 6113 6304 Size: 219 1880 911 384 77 1135 681 191 Cuts at: 6304 826 Size: 1471 Fragments arranged by size: 1880 1471 1135 911 681 384 219 191 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6949 SmaI CCC'GGG Cuts at: 1872 2723 4276 1872 Size: 851 1553 4545 Fragments arranged by size: 4545 1553 851 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 2233 2501 3743 5053 5954 Size: 197 926 193 97 268 1242 1310 901 Cuts at: 5954 6216 820 Size: 262 1553 Fragments arranged by size: 1553 1310 1242 926 901 268 262 197 193 97 SnaBI TAC'GTA Cuts at: 493 4740 493 Size: 4247 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6949 SphI G_CATG'C Cuts at: 1132 4307 1132 Size: 3175 3774 SspI AAT'ATT Cuts at: 5 52 4873 5 Size: 47 4821 2081 Fragments arranged by size: 4821 2081 47 StuI AGG'CCT Cuts at: 1517 2362 2978 3152 1517 Size: 845 616 174 5314 Fragments arranged by size: 5314 845 616 174 StyI C'CwwG_G Cuts at: 513 1065 2004 2574 3011 3054 3070 3348 3564 Size: 552 939 570 437 43 16 278 216 Cuts at: 3564 4388 513 Size: 824 3074 Fragments arranged by size: 3074 939 824 570 552 437 278 216 43 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3674 4976 4993 5146 5331 5776 325 Size: 1255 2094 1302 17 153 185 445 1498 Fragments arranged by size: 2094 1498 1302 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5195 6907 96 Size: 274 80 33 51 1443 3218 1712 138 Fragments arranged by size: 3218 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 6909 6941 2195 Size: 32 4682 32 2203 Fragments arranged by size: 4682 2203 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6949 XbaI T'CTAG_A Cuts at: 4285 4285 Size: 6949 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3346 4132 3346 Size: 786 6163 XhoI C'TCGA_G Cuts at: 1943 3743 1943 Size: 1800 5149 XmaI C'CCGG_G Cuts at: 1870 2721 4274 1870 Size: 851 1553 4545 Fragments arranged by size: 4545 1553 851 XmnI GAAnn'nnTTC Cuts at: 2466 4184 5078 2466 Size: 1718 894 4337 Fragments arranged by size: 4337 1718 894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 276 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI