(Linear) (Six-base) MAPSORT of: KIEE5701.seq Check: 535 from: 1 to: 2244 KIEE5701.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 803 1925 2244 Size: 803 1122 319 Fragments arranged by size: 1122 803 319 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 418 1684 1747 2191 2244 Size: 380 38 1266 63 444 53 Fragments arranged by size: 1266 444 380 63 53 38 AfeI AGC'GCT Cuts at: 0 943 1355 2244 Size: 943 412 889 Fragments arranged by size: 943 889 412 AleI CACnn'nnGTG Cuts at: 0 1091 1912 2244 Size: 1091 821 332 Fragments arranged by size: 1091 821 332 AlwNI CAG_nnn'CTG Cuts at: 0 1626 2244 Size: 1626 618 ApaI G_GGCC'C Cuts at: 0 318 2244 Size: 318 1926 ApoI r'AATT_y Cuts at: 0 256 1777 2244 Size: 256 1521 467 Fragments arranged by size: 1521 467 256 AvaI C'yCGr_G Cuts at: 0 716 1738 1805 1867 2244 Size: 716 1022 67 62 377 Fragments arranged by size: 1022 716 377 67 62 AvrII C'CTAG_G Cuts at: 0 1065 2244 Size: 1065 1179 BamHI G'GATC_C Cuts at: 0 432 2244 Size: 432 1812 BanI G'GyrC_C Cuts at: 0 858 1057 2244 Size: 858 199 1187 Fragments arranged by size: 1187 858 199 BanII G_rGCy'C Cuts at: 0 318 2147 2244 Size: 318 1829 97 Fragments arranged by size: 1829 318 97 BbsI GAAGACnn'nnnn_ Cuts at: 0 1498 1895 2244 Size: 1498 397 349 Fragments arranged by size: 1498 397 349 BglII A'GATC_T Cuts at: 0 1667 2244 Size: 1667 577 BlpI GC'TnA_GC Cuts at: 0 1837 2146 2244 Size: 1837 309 98 Fragments arranged by size: 1837 309 98 Bme1580I G_kGCm'C Cuts at: 0 200 318 1006 2244 Size: 200 118 688 1238 Fragments arranged by size: 1238 688 200 118 BmgBI CAC'GTC Cuts at: 0 152 2244 Size: 152 2092 BmrI ACTGGGnnnn_n' Cuts at: 0 42 2054 2244 Size: 42 2012 190 Fragments arranged by size: 2012 190 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 858 960 1710 2244 Size: 858 102 750 534 Fragments arranged by size: 858 750 534 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 686 1317 1651 2244 Size: 686 631 334 593 Fragments arranged by size: 686 631 593 334 Bpu10I CC'TnA_GC Cuts at: 0 910 2189 2244 Size: 910 1279 55 Fragments arranged by size: 1279 910 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 249 517 2244 Size: 116 133 268 1727 Fragments arranged by size: 1727 268 133 116 BsaI GGTCTCn'nnnn_ Cuts at: 0 418 2244 Size: 418 1826 BsaWI w'CCGG_w Cuts at: 0 1106 2244 Size: 1106 1138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 518 548 2077 2107 2244 Size: 518 30 1529 30 137 Fragments arranged by size: 1529 518 137 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1990 2011 2244 Size: 1990 21 233 Fragments arranged by size: 1990 233 21 BseYI C'CCAG_C Cuts at: 0 64 71 1166 2244 Size: 64 7 1095 1078 Fragments arranged by size: 1095 1078 64 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 496 529 1168 2244 Size: 61 435 33 639 1076 Fragments arranged by size: 1076 639 435 61 33 BsiEI CG_ry'CG Cuts at: 0 1712 2244 Size: 1712 532 BsiHKAI G_wGCw'C Cuts at: 0 1045 2147 2244 Size: 1045 1102 97 Fragments arranged by size: 1102 1045 97 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1956 2244 Size: 1956 288 Bsp1286I G_dGCh'C Cuts at: 0 200 318 1006 1045 2147 2244 Size: 200 118 688 39 1102 97 Fragments arranged by size: 1102 688 200 118 97 39 BsrDI GCAATG_nn' Cuts at: 0 1202 2244 Size: 1202 1042 BsrFI r'CCGG_y Cuts at: 0 32 2244 Size: 32 2212 BssSI C'ACGA_G Cuts at: 0 1308 2244 Size: 1308 936 BstAPI GCAn_nnn'nTGC Cuts at: 0 474 2244 Size: 474 1770 BstEII G'GTnAC_C Cuts at: 0 904 2244 Size: 904 1340 BstYI r'GATC_y Cuts at: 0 432 1667 2244 Size: 432 1235 577 Fragments arranged by size: 1235 577 432 Bsu36I CC'TnA_GG Cuts at: 0 863 2244 Size: 863 1381 BtgI C'CryG_G Cuts at: 0 428 1049 1343 2244 Size: 428 621 294 901 Fragments arranged by size: 901 621 428 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1623 1856 2244 Size: 1623 233 388 Fragments arranged by size: 1623 388 233 BtsI GCAGTG_nn' Cuts at: 0 479 2244 Size: 479 1765 DraIII CAC_nnn'GTG Cuts at: 0 474 2244 Size: 474 1770 DrdI GACnn_nn'nnGTC Cuts at: 0 1168 2244 Size: 1168 1076 EaeI y'GGCC_r Cuts at: 0 997 1046 2039 2244 Size: 997 49 993 205 Fragments arranged by size: 997 993 205 49 EarI CTCTTCn'nnn_ Cuts at: 0 228 346 1541 1603 1679 1967 1975 2185 Size: 228 118 1195 62 76 288 8 210 Cuts at: 2185 2244 Size: 59 Fragments arranged by size: 1195 288 228 210 118 76 62 59 8 EcoICRI GAG'CTC Cuts at: 0 2145 2244 Size: 2145 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 418 686 1317 1651 1684 1747 2191 Size: 380 38 268 631 334 33 63 444 Cuts at: 2191 2244 Size: 53 Fragments arranged by size: 631 444 380 334 268 63 53 38 33 EcoO109I rG'GnC_Cy Cuts at: 0 68 206 314 1717 2244 Size: 68 138 108 1403 527 Fragments arranged by size: 1403 527 138 108 68 EcoRI G'AATT_C Cuts at: 0 1777 2244 Size: 1777 467 EcoRV GAT'ATC Cuts at: 0 225 1404 2244 Size: 225 1179 840 Fragments arranged by size: 1179 840 225 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 267 1423 2196 2244 Size: 267 1156 773 48 Fragments arranged by size: 1156 773 267 48 HaeII r_GCGC'y Cuts at: 0 945 1357 2244 Size: 945 412 887 Fragments arranged by size: 945 887 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 417 449 518 550 565 597 1694 1726 Size: 417 32 69 32 15 32 1097 32 Cuts at: 1726 2077 2109 2244 Size: 351 32 135 Fragments arranged by size: 1097 417 351 135 69 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 0 537 1422 1527 2244 Size: 537 885 105 717 Fragments arranged by size: 885 717 537 105 HindIII A'AGCT_T Cuts at: 0 2235 2244 Size: 2235 9 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 852 1121 1692 2244 Size: 852 269 571 552 Fragments arranged by size: 852 571 552 269 MscI TGG'CCA Cuts at: 0 2041 2244 Size: 2041 203 MslI CAynn'nnrTG Cuts at: 0 845 1091 1124 1540 1657 1750 1912 2244 Size: 845 246 33 416 117 93 162 332 Fragments arranged by size: 845 416 332 246 162 117 93 33 MspA1I CmG'CkG Cuts at: 0 542 2244 Size: 542 1702 NaeI GCC'GGC Cuts at: 0 34 2244 Size: 34 2210 NcoI C'CATG_G Cuts at: 0 1049 1343 2244 Size: 1049 294 901 Fragments arranged by size: 1049 901 294 NdeI CA'TA_TG Cuts at: 0 1658 2244 Size: 1658 586 NgoMIV G'CCGG_C Cuts at: 0 32 2244 Size: 32 2212 PasI CC'CwG_GG Cuts at: 0 1698 2244 Size: 1698 546 PflMI CCAn_nnn'nTGG Cuts at: 0 653 2244 Size: 653 1591 PpuMI rG'GwC_Cy Cuts at: 0 68 206 2244 Size: 68 138 2038 Fragments arranged by size: 2038 138 68 PspOMI G'GGCC_C Cuts at: 0 314 2244 Size: 314 1930 PstI C_TGCA'G Cuts at: 0 924 1835 2219 2244 Size: 924 911 384 25 Fragments arranged by size: 924 911 384 25 PvuII CAG'CTG Cuts at: 0 542 2244 Size: 542 1702 SacI G_AGCT'C Cuts at: 0 2147 2244 Size: 2147 97 SanDI GG'GwC_CC Cuts at: 0 68 2244 Size: 68 2176 SapI GCTCTTCn'nnn_ Cuts at: 0 2185 2244 Size: 2185 59 SfcI C'TryA_G Cuts at: 0 920 1831 2215 2244 Size: 920 911 384 29 Fragments arranged by size: 920 911 384 29 SmaI CCC'GGG Cuts at: 0 718 2244 Size: 718 1526 SmlI C'TyrA_G Cuts at: 0 131 228 496 1738 2244 Size: 131 97 268 1242 506 Fragments arranged by size: 1242 506 268 131 97 StuI AGG'CCT Cuts at: 0 357 973 1147 2244 Size: 357 616 174 1097 Fragments arranged by size: 1097 616 357 174 StyI C'CwwG_G Cuts at: 0 569 1006 1049 1065 1343 1559 2244 Size: 569 437 43 16 278 216 685 Fragments arranged by size: 685 569 437 278 216 43 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1669 2244 Size: 1669 575 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 190 222 2244 Size: 190 32 2022 Fragments arranged by size: 2022 190 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1341 2127 2244 Size: 1341 786 117 Fragments arranged by size: 1341 786 117 XhoI C'TCGA_G Cuts at: 0 1738 2244 Size: 1738 506 XmaI C'CCGG_G Cuts at: 0 716 2244 Size: 716 1528 XmnI GAAnn'nnTTC Cuts at: 0 461 2179 2244 Size: 461 1718 65 Fragments arranged by size: 1718 461 65 Enzymes that do cut: AccI AcuI AfeI AleI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV PasI PflMI PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI SmaI SmlI StuI StyI TaqII TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmtI BsaAI BsaBI BsaHI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BstBI BstXI BstZ17I ClaI CspCI DraI EagI EciI EcoNI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NarI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI ZraI