################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BanI BbeI BbrPI BcgI BciVI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NarI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1201 GTAGAC GT^[AC][GT]AC AccII 4 CGCG CG^CG AcsI 716 GAATTC [GA]^AATT[CT] AcyI 1077 GGCGTC G[GA]^CG[CT]C ApoI 716 GAATTC [GA]^AATT[CT] AspHI 956 GAGCTC G[AT]GC[AT]^C AvaI 167 CTCGAG C^[CT]CG[GA]G AvrII 310 CCTAGG C^CTAGG BamHI 1143 GGATCC G^GATCC BbsI 667 GAAGAC GAAGAC(2/6) BbvCI 1251 GCTGAGG CCTCAGC(-5/-2) BclI 780 TGATCA T^GATCA BcnI 19 CCGGG CC^[GC]GG BlnI 310 CCTAGG C^CTAGG BpuAI 667 GAAGAC GAAGAC(2/6) BsaHI 1077 GGCGTC G[GA]^CG[CT]C BsgI 301 GTGCAG GTGCAG(16/14) BsiHKAI 956 GAGCTC G[AT]GC[AT]^C BsoBI 167 CTCGAG C^[CT]CG[GA]G BstAPI 1111 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 149 CATCC GGATG(2/0) BstUI 4 CGCG CG^CG DraI 1084 TTTAAA TTT^AAA DraIII 698 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 920 CCATGG C^C[GA][CT]GG EarI 831 GAAGAG CTCTTC(1/4) EcoNI 173 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 716 GAATTC G^AATTC FauI 962 CCCGC CCCGC(4/6) FbaI 780 TGATCA T^GATCA FokI 149 CATCC GGATG(9/13) HgaI 1078 GCGTC GACGC(5/10) Hin1I 1077 GGCGTC G[GA]^CG[CT]C HindIII 1168 AAGCTT A^AGCTT Ksp632I 831 GAAGAG CTCTTC(1/4) MaeII 777 ACGT A^CGT MvnI 4 CGCG CG^CG NciI 19 CCGGG CC^[GC]GG NcoI 920 CCATGG C^CATGG NdeI 1282 CATATG CA^TATG NspI 1212 GCATGT [GA]CATG^[CT] PaeR7I 167 CTCGAG C^TCGAG PleI 490 GACTC GAGTC(4/5) PpuMI 1300 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 588 CAGCTG CAG^CTG SacI 956 GAGCTC GAGCT^C SapI 831 GAAGAGC GCTCTTC(1/4) SmlI 167 CTCGAG C^T[CT][GA]AG SspI 1088 AATATT AAT^ATT SwaI 1083 ATTTAAAT ATTT^AAAT TaqI 168 TCGA T^CGA TthHB8I 168 TCGA T^CGA XhoI 167 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BalI 386 TGGCCA TGG^CCA 1001 TGGCCA BanII 351 GAGCCC G[GA]GC[CT]^C 956 GAGCTC BglI 306 GCCTCCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1120 GCCATTGAGGC BmyI 351 GAGCCC G[AGT]GC[ACT]^C 956 GAGCTC BpmI 67 CTGGAG CTGGAG(16/14) 332 CTGGAG BseRI 1025 CTCCTC GAGGAG(10/8) 13 GAGGAG BsiYI 173 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 963 CCGCAGCGTGG BslI 173 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 963 CCGCAGCGTGG BsmFI 362 GTCCC GGGAC(10/14) 564 GTCCC Bsp1286I 351 GAGCCC G[AGT]GC[ACT]^C 956 GAGCTC BsrFI 60 GCCGGC [GA]^CCGG[CT] 1263 GCCGGC BstYI 194 GGATCT [GA]^GATC[CT] 1143 GGATCC CfoI 3 GCGC GCG^C 1210 GCGC Cfr10I 60 GCCGGC [GA]^CCGG[CT] 1263 GCCGGC DraII 21 GGGGCCT [GA]G^G[ACGT]CC[CT] 1300 GGGTCCT EcoO109I 21 GGGGCCT [GA]G^G[ACGT]CC[CT] 1300 GGGTCCT HhaI 3 GCGC GCG^C 1210 GCGC HinP1I 3 GCGC G^CGC 1210 GCGC MflI 194 GGATCT [GA]^GATC[CT] 1143 GGATCC MluNI 386 TGGCCA TGG^CCA 1001 TGGCCA MscI 386 TGGCCA TGG^CCA 1001 TGGCCA MspA1I 588 CAGCTG C[AC]G^C[GT]G 1139 CAGCGG NaeI 60 GCCGGC GCC^GGC 1263 GCCGGC NgoMIV 60 GCCGGC G^CCGGC 1263 GCCGGC SfcI 32 CTACAG C^T[GA][CT]AG 1199 CTGTAG XhoII 194 GGATCT [GA]^GATC[CT] 1143 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BfaI BsaJI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI