(Circular) (Six-base) MAPSORT of: pFN21AE5517.seq Check: 4118 from: 1 to: 6028 pFN21AE5517 6028 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3207 3371 1937 Size: 1270 164 4594 Fragments arranged by size: 4594 1270 164 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2667 2691 4091 5475 5743 1632 Size: 1035 24 1400 1384 268 1917 Fragments arranged by size: 1917 1400 1384 1035 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 4927 5904 1203 Size: 3724 977 1327 Fragments arranged by size: 3724 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 3771 5343 3771 Size: 1572 4456 ApaLI G'TGCA_C Cuts at: 4085 5241 4085 Size: 1156 4872 ApoI r'AATT_y Cuts at: 1493 2721 3337 3563 3715 5824 1493 Size: 1228 616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1228 616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3353 1870 Size: 73 229 1181 4545 Fragments arranged by size: 4545 1181 229 73 AvrII C'CTAG_G Cuts at: 2315 2315 Size: 6028 BamHI G'GATC_C Cuts at: 3148 3358 3725 3148 Size: 210 367 5451 Fragments arranged by size: 5451 367 210 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3349 4804 618 Size: 325 217 627 1562 1455 1842 Fragments arranged by size: 1842 1562 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2360 2965 3347 1391 Size: 969 605 382 4072 Fragments arranged by size: 4072 969 605 382 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2679 928 Size: 971 780 4277 Fragments arranged by size: 4277 971 780 BbvCI CC'TCA_GC Cuts at: 3257 3257 Size: 6028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4219 4253 1903 Size: 34 50 34 2198 34 3678 Fragments arranged by size: 3678 2198 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 2785 1548 Size: 1237 4791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2317 3131 4639 Size: 107 122 71 631 605 645 814 1508 Cuts at: 4639 136 Size: 1525 Fragments arranged by size: 1525 1508 814 645 631 605 122 107 71 BglII A'GATC_T Cuts at: 6023 6023 Size: 6028 BlpI GC'TnA_GC Cuts at: 3445 3445 Size: 6028 Bme1580I G_kGCm'C Cuts at: 1790 4089 5245 1790 Size: 2299 1156 2573 Fragments arranged by size: 2573 2299 1156 BmgBI CAC'GTC Cuts at: 1318 2783 1318 Size: 1465 4563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2566 3759 4717 Size: 416 53 83 399 24 1149 1193 958 Cuts at: 4717 442 Size: 1753 Fragments arranged by size: 1753 1193 1149 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2358 4688 1239 Size: 854 265 2330 2579 Fragments arranged by size: 2579 2330 854 265 Bpu10I CC'TnA_GC Cuts at: 3257 3257 Size: 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4153 5018 5316 4153 Size: 865 298 4865 Fragments arranged by size: 4865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4691 882 Size: 617 3192 2219 Fragments arranged by size: 3192 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3083 3835 4217 4852 Size: 53 83 186 936 1550 752 382 635 Cuts at: 4852 275 Size: 1451 Fragments arranged by size: 1550 1451 936 752 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4461 4859 5133 5280 5691 4461 Size: 398 274 147 411 4798 Fragments arranged by size: 4798 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2877 2907 2983 3013 1178 Size: 30 165 30 1474 30 76 30 4193 Fragments arranged by size: 4193 1474 165 76 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3021 1186 Size: 847 988 4193 Fragments arranged by size: 4193 988 847 BseYI C'CCAG_C Cuts at: 5231 5727 5231 Size: 496 5532 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 1163 Size: 573 591 4864 Fragments arranged by size: 4864 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 4239 5267 1540 Size: 275 186 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2965 4089 4174 5245 2965 Size: 1124 85 1071 3748 Fragments arranged by size: 3748 1124 1071 85 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 2965 3347 4089 4174 5245 1391 Size: 399 570 605 382 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 605 570 399 382 85 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3383 844 Size: 2539 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3915 1394 Size: 429 2092 3507 Fragments arranged by size: 3507 2092 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4523 4697 65 Size: 1183 3275 174 1396 Fragments arranged by size: 3275 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 3268 4672 4859 1700 Size: 110 255 1203 1404 187 2869 Fragments arranged by size: 2869 1404 1203 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 4088 5100 4088 Size: 1012 5016 BstAPI GCAn_nnn'nTGC Cuts at: 794 3122 794 Size: 2328 3700 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2199 3148 3358 3725 4110 4127 5579 5665 Size: 227 949 210 367 385 17 1452 86 Cuts at: 5665 5677 6023 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 949 385 367 346 227 210 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2925 513 Size: 552 1860 3616 Fragments arranged by size: 3616 1860 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 2763 2935 3544 4338 4358 2763 Size: 172 609 794 20 4433 Fragments arranged by size: 4433 794 609 172 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 3091 3332 3091 Size: 241 5787 DraIII CAC_nnn'GTG Cuts at: 2708 2708 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2391 3006 Size: 54 1425 50 275 18 297 264 615 Cuts at: 3006 3475 4364 5993 8 Size: 469 889 1629 43 Fragments arranged by size: 1629 1425 889 615 469 297 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3475 1537 Size: 275 1663 4090 Fragments arranged by size: 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3958 1353 Size: 1478 1127 3423 Fragments arranged by size: 3423 1478 1127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4615 4989 5135 387 Size: 1064 138 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2963 2963 Size: 6028 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2358 2667 2691 4091 4688 5475 Size: 393 461 265 309 24 1400 597 787 Cuts at: 5475 5743 1239 Size: 268 1524 Fragments arranged by size: 1524 1400 787 597 461 393 309 268 265 24 EcoNI CCTnn'n_nnAGG Cuts at: 2182 2182 Size: 6028 EcoO109I rG'GnC_Cy Cuts at: 2027 3306 2027 Size: 1279 4749 EcoRI G'AATT_C Cuts at: 2721 3337 3715 2721 Size: 616 378 5034 Fragments arranged by size: 5034 616 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 6028 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 5175 1678 Size: 3497 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2347 2379 2877 Size: 32 135 32 496 32 247 32 498 Cuts at: 2877 2909 2983 3015 3140 3172 4669 4701 4743 Size: 32 74 32 125 32 1497 32 42 Cuts at: 4743 4775 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 498 496 247 135 125 74 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3372 3618 669 Size: 988 281 1434 246 3079 Fragments arranged by size: 3079 1434 988 281 246 HindIII A'AGCT_T Cuts at: 748 3173 748 Size: 2425 3603 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5142 5326 1305 Size: 3837 184 2007 Fragments arranged by size: 3837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 3008 10 Size: 54 1425 343 561 615 3030 Fragments arranged by size: 3030 1425 615 561 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3986 4345 4504 518 Size: 648 2820 359 159 2042 Fragments arranged by size: 2820 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2595 3146 3444 4121 5269 5514 1606 Size: 989 551 298 677 1148 245 2120 Fragments arranged by size: 2120 1148 989 677 551 298 245 NaeI GCC'GGC Cuts at: 2067 3270 2067 Size: 1203 4825 NcoI C'CATG_G Cuts at: 513 1065 2925 513 Size: 552 1860 3616 Fragments arranged by size: 3616 1860 552 NdeI CA'TA_TG Cuts at: 387 3288 387 Size: 2901 3127 NgoMIV G'CCGG_C Cuts at: 2065 3268 2065 Size: 1203 4825 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 3221 3386 4931 1132 Size: 97 1992 165 1545 2229 Fragments arranged by size: 2229 1992 1545 165 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6028 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4129 5610 5642 4097 Size: 32 1481 32 4483 Fragments arranged by size: 4483 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3306 3306 Size: 6028 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 3598 3598 Size: 6028 PstI C_TGCA'G Cuts at: 830 3380 830 Size: 2550 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 PvuII CAG'CTG Cuts at: 2595 2595 Size: 6028 SacI G_AGCT'C Cuts at: 2965 2965 Size: 6028 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SapI GCTCTTCn'nnn_ Cuts at: 2831 2831 Size: 6028 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SfcI C'TryA_G Cuts at: 826 1045 2037 3204 3376 4511 5192 5383 826 Size: 219 992 1167 172 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 992 681 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 3355 1872 Size: 1483 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2172 4132 5033 5295 820 Size: 197 926 229 1960 901 262 1553 Fragments arranged by size: 1960 1553 926 901 262 229 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 3095 3952 5 Size: 47 3043 857 2081 Fragments arranged by size: 3043 2081 857 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6028 StyI C'CwwG_G Cuts at: 513 1065 2315 2925 3049 3309 3467 513 Size: 552 1250 610 124 260 158 3074 Fragments arranged by size: 3074 1250 610 552 260 158 124 SwaI ATTT'AAAT Cuts at: 3091 3091 Size: 6028 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2977 3144 4055 4072 4225 4410 4855 Size: 1255 1397 167 911 17 153 185 445 Cuts at: 4855 325 Size: 1498 Fragments arranged by size: 1498 1397 1255 911 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4274 5986 96 Size: 274 80 33 51 1443 2297 1712 138 Fragments arranged by size: 2297 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5988 6020 5988 Size: 32 5996 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6028 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 2172 1943 Size: 229 5799 XmaI C'CCGG_G Cuts at: 1870 3353 1870 Size: 1483 4545 XmnI GAAnn'nnTTC Cuts at: 4157 4157 Size: 6028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI