Restriction map for
pFN21AE5517
(Linear) (Six-base) MAPSORT of: KIEE5517.seq Check: 4094 from: 1 to: 1323
KIEE5517.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AccI GT'mk_AC
Cuts at: 0 1202 1323
Size: 1202 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 686 1323
Size: 662 24 637
Fragments arranged by size:
662 637 24
ApoI r'AATT_y
Cuts at: 0 716 1323
Size: 716 607
AvaI C'yCGr_G
Cuts at: 0 167 1323
Size: 167 1156
AvrII C'CTAG_G
Cuts at: 0 310 1323
Size: 310 1013
BamHI G'GATC_C
Cuts at: 0 1143 1323
Size: 1143 180
BanII G_rGCy'C
Cuts at: 0 355 960 1323
Size: 355 605 363
Fragments arranged by size:
605 363 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 674 1323
Size: 674 649
BbvCI CC'TCA_GC
Cuts at: 0 1252 1323
Size: 1252 71
BclI T'GATC_A
Cuts at: 0 780 1323
Size: 780 543
BglI GCCn_nnn'nGGC
Cuts at: 0 312 1126 1323
Size: 312 814 197
Fragments arranged by size:
814 312 197
BmgBI CAC'GTC
Cuts at: 0 778 1323
Size: 778 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 1323
Size: 561 762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 507 1323
Size: 507 816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 353 1323
Size: 88 265 970
Fragments arranged by size:
970 265 88
Bpu10I CC'TnA_GC
Cuts at: 0 1252 1323
Size: 1252 71
BsaHI Gr'CG_yC
Cuts at: 0 1078 1323
Size: 1078 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 872 902 978 1008 1323
Size: 872 30 76 30 315
Fragments arranged by size:
872 315 76 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1016 1323
Size: 28 988 307
Fragments arranged by size:
988 307 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1323
Size: 322 1001
BsiHKAI G_wGCw'C
Cuts at: 0 960 1323
Size: 960 363
Bsp1286I G_dGCh'C
Cuts at: 0 355 960 1323
Size: 355 605 363
Fragments arranged by size:
605 363 355
BsrFI r'CCGG_y
Cuts at: 0 60 1263 1323
Size: 60 1203 60
Fragments arranged by size:
1203 60 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1117 1323
Size: 1117 206
BstYI r'GATC_y
Cuts at: 0 194 1143 1323
Size: 194 949 180
Fragments arranged by size:
949 194 180
BtgI C'CryG_G
Cuts at: 0 920 1323
Size: 920 403
BtsI GCAGTG_nn'
Cuts at: 0 758 930 1323
Size: 758 172 393
Fragments arranged by size:
758 393 172
DraI TTT'AAA
Cuts at: 0 1086 1323
Size: 1086 237
DraIII CAC_nnn'GTG
Cuts at: 0 703 1323
Size: 703 620
EaeI y'GGCC_r
Cuts at: 0 122 386 1001 1323
Size: 122 264 615 322
Fragments arranged by size:
615 322 264 122
EarI CTCTTCn'nnn_
Cuts at: 0 826 1323
Size: 826 497
EcoICRI GAG'CTC
Cuts at: 0 958 1323
Size: 958 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 353 662 686 1323
Size: 88 265 309 24 637
Fragments arranged by size:
637 309 265 88 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 177 1323
Size: 177 1146
EcoO109I rG'GnC_Cy
Cuts at: 0 22 1301 1323
Size: 22 1279 22
Fragments arranged by size:
1279 22 22
EcoRI G'AATT_C
Cuts at: 0 716 1323
Size: 716 607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 213 1323
Size: 213 1110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 342 374 872 904 978 1010
Size: 63 32 247 32 498 32 74 32
Cuts at: 1010 1135 1167 1323
Size: 125 32 156
Fragments arranged by size:
498 247 156 125 74 63 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1168 1323
Size: 1168 155
MscI TGG'CCA
Cuts at: 0 388 1003 1323
Size: 388 615 320
Fragments arranged by size:
615 388 320
MspA1I CmG'CkG
Cuts at: 0 590 1141 1323
Size: 590 551 182
Fragments arranged by size:
590 551 182
NaeI GCC'GGC
Cuts at: 0 62 1265 1323
Size: 62 1203 58
Fragments arranged by size:
1203 62 58
NcoI C'CATG_G
Cuts at: 0 920 1323
Size: 920 403
NdeI CA'TA_TG
Cuts at: 0 1283 1323
Size: 1283 40
NgoMIV G'CCGG_C
Cuts at: 0 60 1263 1323
Size: 60 1203 60
Fragments arranged by size:
1203 60 60
NspI r_CATG'y
Cuts at: 0 1216 1323
Size: 1216 107
PpuMI rG'GwC_Cy
Cuts at: 0 1301 1323
Size: 1301 22
PvuII CAG'CTG
Cuts at: 0 590 1323
Size: 590 733
SacI G_AGCT'C
Cuts at: 0 960 1323
Size: 960 363
SapI GCTCTTCn'nnn_
Cuts at: 0 826 1323
Size: 826 497
SfcI C'TryA_G
Cuts at: 0 32 1199 1323
Size: 32 1167 124
Fragments arranged by size:
1167 124 32
SmlI C'TyrA_G
Cuts at: 0 167 1323
Size: 167 1156
SspI AAT'ATT
Cuts at: 0 1090 1323
Size: 1090 233
StyI C'CwwG_G
Cuts at: 0 310 920 1044 1304 1323
Size: 310 610 124 260 19
Fragments arranged by size:
610 310 260 124 19
SwaI ATTT'AAAT
Cuts at: 0 1086 1323
Size: 1086 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 972 1139 1323
Size: 972 167 184
Fragments arranged by size:
972 184 167
XhoI C'TCGA_G
Cuts at: 0 167 1323
Size: 167 1156
Enzymes that do cut:
AccI AcuI ApoI AvaI AvrII BamHI BanII BbsI
BbvCI BclI BglI BmgBI BmrI BplI BpmI Bpu10I
BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrFI BstAPI
BstYI BtgI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HindIII MscI
MspA1I NaeI NcoI NdeI NgoMIV NspI PpuMI PvuII
SacI SapI SfcI SmlI SspI StyI SwaI TaqII
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI BaeI BanI BbeI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmtI BpuEI BsaI BsaAI BsaBI
BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI
EciI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MslI NarI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI