################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BstBI BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HapII Hin1I HincII HindII HpaI HpaII ItaI KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 1284 CCGC CCGC(-3/-1) Asp700I 1196 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 689 TGGCCA TGG^CCA BamHI 875 GGATCC G^GATCC BmyI 77 GGGCAC G[AGT]GC[ACT]^C BpmI 600 CTCCAG CTGGAG(16/14) BseRI 356 GAGGAG GAGGAG(10/8) BsmI 992 GAATGC GAATGC(1/-1) Bsp1286I 77 GGGCAC G[AGT]GC[ACT]^C BssSI 8 CTCGTG CACGAG(-5/-1) Bst1107I 985 GTATAC GTA^TAC BstAPI 841 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 985 GTATAC GTA^TAC CfoI 51 GCGC GCG^C DraI 1027 TTTAAA TTT^AAA DraII 386 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1042 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 871 CCGTGG C^C[GA][CT]GG EaeI 689 TGGCCA [CT]^GGCC[GA] EarI 271 GAAGAG CTCTTC(1/4) EcoO109I 386 GGGTCCC [GA]G^G[ACGT]CC[CT] HaeII 50 AGCGCC [GA]GCGC^[CT] HgaI 544 GACGC GACGC(5/10) HhaI 51 GCGC GCG^C HinP1I 51 GCGC G^CGC HindIII 1032 AAGCTT A^AGCTT Ksp632I 271 GAAGAG CTCTTC(1/4) MluNI 689 TGGCCA TGG^CCA MscI 689 TGGCCA TGG^CCA MspA1I 457 CAGCTG C[AC]G^C[GT]G NheI 3 GCTAGC G^CTAGC PpuMI 386 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 457 CAGCTG CAG^CTG SfcI 480 CTACAG C^T[GA][CT]AG SmlI 622 CTCAAG C^T[CT][GA]AG TaqI 1214 TCGA T^CGA TthHB8I 1214 TCGA T^CGA XmnI 1196 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 541 GTAGAC GT^[AC][GT]AC 985 GTATAC AvaII 195 GGACC G^G[AT]CC 387 GGTCC BbsI 666 GAAGAC GAAGAC(2/6) 964 GTCTTC BpuAI 666 GAAGAC GAAGAC(2/6) 964 GTCTTC BsgI 571 CTGCAC GTGCAG(16/14) 734 CTGCAC BsmFI 430 GGGAC GGGAC(10/14) 388 GTCCC Bsp1407I 999 TGTACA T^GTACA 1267 TGTACA BsrGI 999 TGTACA T^GTACA 1267 TGTACA BstF5I 554 CATCC GGATG(2/0) 1232 CATCC BstXI 241 CCAGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 391 CCAGAGTGGTGG BstYI 301 GGATCT [GA]^GATC[CT] 875 GGATCC Cac8I 3 GCTAGC GC[ACGT]^[ACGT]GC 402 GCTTGC EcoRV 719 GATATC GAT^ATC 1111 GATATC FokI 554 CATCC GGATG(9/13) 1232 CATCC HphI 14 TCACC GGTGA(8/7) 515 TCACC MaeII 30 ACGT A^CGT 1076 ACGT MflI 301 GGATCT [GA]^GATC[CT] 875 GGATCC NspI 639 ACATGC [GA]CATG^[CT] 886 GCATGT SspBI 999 TGTACA T^GTACA 1267 TGTACA XcmI 241 CCAGCCTTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 744 CCACAGCAGTTTTGG XhoII 301 GGATCT [GA]^GATC[CT] 875 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BfaI BsaJI BsiYI BslI BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HinfI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I TspRI