(Linear) (Six-base) MAPSORT of: KIEE4423.seq  Check: 3130  from: 1  to: 1314



KIEE4423.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:58  ..

AccI GT'mk_AC

Cuts at:      0     542     986    1314 
   Size:        542     444     328

  Fragments arranged by size: 

                 542     444     328
 
AleI CACnn'nnGTG

Cuts at:      0     659     661    1314 
   Size:        659       2     653

  Fragments arranged by size: 

                 659     653       2
 
ApoI r'AATT_y

Cuts at:      0     114     316    1158    1314 
   Size:        114     202     842     156

  Fragments arranged by size: 

                 842     202     156     114
 
BamHI G'GATC_C

Cuts at:      0     875    1314 
   Size:        875     439
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     673     957    1314 
   Size:        673     284     357

  Fragments arranged by size: 

                 673     357     284
 
Bme1580I G_kGCm'C

Cuts at:      0      81    1314 
   Size:         81    1233
 
BmgBI CAC'GTC

Cuts at:      0    1077    1314 
   Size:       1077     237
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     163     307    1314 
   Size:        163     144    1007

  Fragments arranged by size: 

                1007     163     144
 
BmtI G_CTAG'C

Cuts at:      0       7    1314 
   Size:          7    1307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     585    1314 
   Size:        585     729
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1314 
   Size:        607     707
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     350     380     875     905    1314 
   Size:        350      30     495      30     409

  Fragments arranged by size: 

                 495     409     350      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     371    1314 
   Size:        371     943
 
BseYI C'CCAG_C

Cuts at:      0     534     836    1054    1314 
   Size:        534     302     218     260

  Fragments arranged by size: 

                 534     302     260     218
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     719    1314 
   Size:        556     163     595

  Fragments arranged by size: 

                 595     556     163
 
BsmI GAATG_Cn'

Cuts at:      0     998    1314 
   Size:        998     316
 
Bsp1286I G_dGCh'C

Cuts at:      0      81    1314 
   Size:         81    1233
 
BsrGI T'GTAC_A

Cuts at:      0     999    1267    1314 
   Size:        999     268      47

  Fragments arranged by size: 

                 999     268      47
 
BssSI C'ACGA_G

Cuts at:      0       8    1314 
   Size:          8    1306
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     847    1314 
   Size:        847     467
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     248     398    1314 
   Size:        248     150     916

  Fragments arranged by size: 

                 916     248     150
 
BstYI r'GATC_y

Cuts at:      0     301     875    1314 
   Size:        301     574     439

  Fragments arranged by size: 

                 574     439     301
 
BstZ17I GTA'TAC

Cuts at:      0     987    1314 
   Size:        987     327
 
BtgI C'CryG_G

Cuts at:      0     871    1314 
   Size:        871     443
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     294    1314 
   Size:        294    1020
 
BtsI GCAGTG_nn'

Cuts at:      0      79    1314 
   Size:         79    1235
 
DraI TTT'AAA

Cuts at:      0    1029    1314 
   Size:       1029     285
 
DraIII CAC_nnn'GTG

Cuts at:      0    1047    1314 
   Size:       1047     267
 
EaeI y'GGCC_r

Cuts at:      0     689    1314 
   Size:        689     625
 
EarI CTCTTCn'nnn_

Cuts at:      0     266    1314 
   Size:        266    1048
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     585    1314 
   Size:        585     729
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    1314 
   Size:        387     927
 
EcoRV GAT'ATC

Cuts at:      0     721    1113    1314 
   Size:        721     392     201

  Fragments arranged by size: 

                 721     392     201
 
HaeII r_GCGC'y

Cuts at:      0      54    1314 
   Size:         54    1260
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      15      47    1314 
   Size:         15      32    1267

  Fragments arranged by size: 

                1267      32      15
 
HindIII A'AGCT_T

Cuts at:      0    1032    1314 
   Size:       1032     282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1314 
   Size:        146    1168
 
MscI TGG'CCA

Cuts at:      0     691    1314 
   Size:        691     623
 
MslI CAynn'nnrTG

Cuts at:      0     558     644     659     661    1314 
   Size:        558      86      15       2     653

  Fragments arranged by size: 

                 653     558      86      15       2
 
MspA1I CmG'CkG

Cuts at:      0     459    1314 
   Size:        459     855
 
NheI G'CTAG_C

Cuts at:      0       3    1314 
   Size:          3    1311
 
NspI r_CATG'y

Cuts at:      0     643     890    1314 
   Size:        643     247     424

  Fragments arranged by size: 

                 643     424     247
 
PasI CC'CwG_GG

Cuts at:      0     383    1314 
   Size:        383     931
 
PfoI T'CCnGG_A

Cuts at:      0     191     444    1240    1314 
   Size:        191     253     796      74

  Fragments arranged by size: 

                 796     253     191      74
 
PpuMI rG'GwC_Cy

Cuts at:      0     387    1314 
   Size:        387     927
 
PvuII CAG'CTG

Cuts at:      0     459    1314 
   Size:        459     855
 
SanDI GG'GwC_CC

Cuts at:      0     387    1314 
   Size:        387     927
 
SfcI C'TryA_G

Cuts at:      0     480    1314 
   Size:        480     834
 
SmlI C'TyrA_G

Cuts at:      0     622    1314 
   Size:        622     692
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     757    1314 
   Size:        757     557
 
TatI w'GTAC_w

Cuts at:      0     999    1267    1314 
   Size:        999     268      47

  Fragments arranged by size: 

                 999     268      47
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     248     751    1314 
   Size:        248     503     563

  Fragments arranged by size: 

                 563     503     248
 
XmnI GAAnn'nnTTC

Cuts at:      0    1200    1314 
   Size:       1200     114
 

 Enzymes that do cut:

     AccI     AleI     ApoI    BamHI     BbsI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI    BsaXI    BseRI    BseYI     BsgI     BsmI
 Bsp1286I    BsrGI    BssSI   BstAPI    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I
    EcoRV    HaeII    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I
     NheI     NspI     PasI     PfoI    PpuMI    PvuII    SanDI     SfcI
     SmlI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BstBI   BstEII   Bsu36I     ClaI    CspCI     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI