(Circular) (Six-base) MAPSORT of: pFN21AE2723.seq Check: 8435 from: 1 to: 5611 pFN21AE2723 5611 bp With 182 enzymes: SgfI * July 7, 2018 16:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2891 2891 Size: 5611 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2747 3674 5058 5326 1632 Size: 620 495 927 1384 268 1917 Fragments arranged by size: 1917 1384 927 620 495 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 4510 5487 1203 Size: 3307 977 1327 Fragments arranged by size: 3307 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 2651 1166 Size: 1485 4126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 3354 4926 3354 Size: 1572 4039 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2864 2920 3146 3298 5407 1493 Size: 1371 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 1371 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2849 2936 1870 Size: 73 906 87 4545 Fragments arranged by size: 4545 906 87 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 2555 2932 4387 618 Size: 325 217 627 402 366 377 1455 1842 Fragments arranged by size: 1842 1455 627 402 377 366 325 217 BanII G_rGCy'C Cuts at: 1391 2930 1391 Size: 1539 4072 BbeI G_GCGC'C Cuts at: 2193 2193 Size: 5611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BfrBI ATG'CAT Cuts at: 2806 2806 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4222 136 Size: 107 122 71 631 605 2550 1525 Fragments arranged by size: 2550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 2267 3672 4828 1790 Size: 477 1405 1156 2573 Fragments arranged by size: 2573 1405 1156 477 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3342 4300 442 Size: 416 53 83 399 24 1925 958 1753 Fragments arranged by size: 1925 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 4271 1239 Size: 1274 1758 2579 Fragments arranged by size: 2579 1758 1274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 3736 4601 4899 2411 Size: 1325 865 298 3123 Fragments arranged by size: 3123 1325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4274 882 Size: 617 2775 2219 Fragments arranged by size: 2775 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2158 2190 3418 3800 Size: 53 83 186 936 625 32 1228 382 Cuts at: 3800 4435 275 Size: 635 1451 Fragments arranged by size: 1451 1228 936 635 625 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2297 2327 1178 Size: 30 165 30 894 30 4462 Fragments arranged by size: 4462 894 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2321 2414 2774 2777 2780 2867 1186 Size: 1075 60 93 360 3 3 87 3930 Fragments arranged by size: 3930 1075 360 93 87 60 3 3 BseYI C'CCAG_C Cuts at: 2020 4814 5310 2020 Size: 2794 496 2321 Fragments arranged by size: 2794 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 2786 2829 1163 Size: 573 818 232 43 3945 Fragments arranged by size: 3945 818 573 232 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3672 3757 4828 3672 Size: 85 1071 4455 Fragments arranged by size: 4455 1071 85 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 BsmBI CGTCTCn'nnnn_ Cuts at: 2231 2729 2231 Size: 498 5113 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 2930 3672 3757 4828 1391 Size: 399 477 663 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 663 477 399 85 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 2966 844 Size: 2047 75 3489 Fragments arranged by size: 3489 2047 75 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 4255 4442 1700 Size: 110 325 2120 187 2869 Fragments arranged by size: 2869 2120 325 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 2025 1925 Size: 100 5511 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 2271 2543 794 Size: 1477 272 3862 Fragments arranged by size: 3862 1477 272 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2250 2856 2941 3308 3693 3710 5162 5248 Size: 278 606 85 367 385 17 1452 86 Cuts at: 5248 5260 5606 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 606 385 367 346 278 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2379 2386 2795 513 Size: 552 939 375 7 409 3329 Fragments arranged by size: 3329 939 552 409 375 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 3127 3921 3941 3127 Size: 794 20 4797 Fragments arranged by size: 4797 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DraIII CAC_nnn'GTG Cuts at: 2271 2515 2271 Size: 244 5367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2506 3058 Size: 54 1425 50 275 18 492 184 552 Cuts at: 3058 3947 5576 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 552 492 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 3541 1353 Size: 1034 1154 3423 Fragments arranged by size: 3423 1154 1034 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2920 4198 4572 4718 387 Size: 1064 138 1331 1278 374 146 1280 Fragments arranged by size: 1331 1280 1278 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2513 2747 3674 4271 5058 5326 Size: 393 620 261 234 927 597 787 268 Cuts at: 5326 1239 Size: 1524 Fragments arranged by size: 1524 927 787 620 597 393 268 261 234 EcoO109I rG'GnC_Cy Cuts at: 2107 2779 2107 Size: 672 4939 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2193 2646 4758 1678 Size: 515 453 2112 2531 Fragments arranged by size: 2531 2112 515 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2041 2058 2073 4252 Size: 32 135 32 454 15 17 15 2179 Cuts at: 4252 4284 4326 4358 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2179 454 135 42 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KasI G'GCGC_C Cuts at: 2189 2189 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2059 4725 4909 1305 Size: 754 2666 184 2007 Fragments arranged by size: 2666 2007 754 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2508 10 Size: 54 1425 343 676 3113 Fragments arranged by size: 3113 1425 676 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2651 2678 2885 3569 3928 4087 Size: 648 1197 288 27 207 684 359 159 Cuts at: 4087 518 Size: 2042 Fragments arranged by size: 2042 1197 684 648 359 288 207 159 27 MspA1I CmG'CkG Cuts at: 1606 3027 3704 4852 5097 1606 Size: 1421 677 1148 245 2120 Fragments arranged by size: 2120 1421 1148 677 245 NarI GG'CG_CC Cuts at: 2190 2190 Size: 5611 NcoI C'CATG_G Cuts at: 513 1065 2004 2386 2795 513 Size: 552 939 382 409 3329 Fragments arranged by size: 3329 939 552 409 382 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NsiI A_TGCA'T Cuts at: 2808 2808 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2810 2969 4514 1132 Size: 97 1581 159 1545 2229 Fragments arranged by size: 2229 1581 1545 159 97 PasI CC'CwG_GG Cuts at: 1348 2449 2614 1348 Size: 1101 165 4345 Fragments arranged by size: 4345 1101 165 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4709 PfoI T'CCnGG_A Cuts at: 2334 2632 2334 Size: 298 5313 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2107 Size: 5611 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PspXI vC'TCGA_Gb Cuts at: 2849 2849 Size: 5611 PstI C_TGCA'G Cuts at: 830 2502 2963 830 Size: 1672 461 3478 Fragments arranged by size: 3478 1672 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2387 2387 Size: 5611 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2045 2498 2959 4094 4775 4966 826 Size: 219 1000 453 461 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 681 461 453 219 191 SfoI GGC'GCC Cuts at: 2191 2191 Size: 5611 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 2135 1700 Size: 435 5176 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 2849 3715 4616 4878 820 Size: 197 926 483 423 866 901 262 1553 Fragments arranged by size: 1553 926 901 866 483 423 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2810 2969 1132 Size: 1678 159 3774 Fragments arranged by size: 3774 1678 159 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 4654 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 2386 2469 2795 3050 513 Size: 552 939 321 61 83 326 255 3074 Fragments arranged by size: 3074 939 552 326 321 255 83 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2336 3638 3655 3808 3993 4438 325 Size: 1255 756 1302 17 153 185 445 1498 Fragments arranged by size: 1498 1302 1255 756 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3857 5569 96 Size: 274 80 33 51 1443 1880 1712 138 Fragments arranged by size: 1880 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 2849 1943 Size: 906 4705 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 2894 3740 2894 Size: 846 4765 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI