################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AhdI 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 381 GATCC GGATC(4/5) AlwNI 117 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 5 CTCGGG C^[CT]CG[GA]G AvaII 132 GGACC G^G[AT]CC BanI 94 GGCACC G^G[CT][GA]CC BcnI 77 CCGGG CC^[GC]GG BsaI 346 GAGACC GGTCTC(1/5) BsiEI 172 CGACCG CG[GA][CT]^CG BsmBI 303 GAGACG CGTCTC(1/5) BsoBI 5 CTCGGG C^[CT]CG[GA]G BssSI 37 CTCGTG CACGAG(-5/-1) BstF5I 240 CATCC GGATG(2/0) DdeI 256 CTGAG C^T[ACGT]AG DpnII 381 GATC ^GATC EaeI 74 TGGCCG [CT]^GGCC[GA] Eam1105I 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 243 CCTTGG C^C[AT][AT]GG FauI 110 CCCGC CCCGC(4/6) FokI 240 CATCC GGATG(9/13) HgaI 157 GCGTC GACGC(5/10) HphI 329 TCACC GGTGA(8/7) KspI 111 CCGCGG CCGC^GG MboI 381 GATC ^GATC NciI 77 CCGGG CC^[GC]GG NdeII 381 GATC ^GATC NlaIII 147 CATG CATG^ PstI 203 CTGCAG CTGCA^G PvuII 368 CAGCTG CAG^CTG SacII 111 CCGCGG CCGC^GG Sau3AI 381 GATC ^GATC SfaNI 308 GATGC GCATC(5/9) SfcI 203 CTGCAG C^T[GA][CT]AG StyI 243 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 118 AGCT AG^CT 369 AGCT BanII 9 GGGCTC G[GA]GC[CT]^C 46 GGGCTC BmyI 9 GGGCTC G[AGT]GC[ACT]^C 46 GGGCTC BpmI 123 CTGGAG CTGGAG(16/14) 300 CTGGAG BseRI 34 CTCCTC GAGGAG(10/8) 343 GAGGAG BsmAI 303 GAGAC GTCTC(1/5) 346 GAGAC Bsp1286I 9 GGGCTC G[AGT]GC[ACT]^C 46 GGGCTC BsrFI 20 GCCGGT [GA]^CCGG[CT] 349 ACCGGC BstAPI 146 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 208 GCACCTCCTGC Cfr10I 20 GCCGGT [GA]^CCGG[CT] 349 ACCGGC Cfr13I 132 GGACC G^G[ACGT]CC 236 GGCCC DsaI 71 CCGTGG C^C[GA][CT]GG 111 CCGCGG HaeIII 75 GGCC GG^CC 236 GGCC MspA1I 111 CCGCGG C[AC]G^C[GT]G 368 CAGCTG NlaIV 32 GGCTCC GG[ACGT]^[ACGT]CC 94 GGCACC Sau96I 132 GGACC G^G[ACGT]CC 236 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI BbvI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI EcoRII Fnu4HI HaeII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI ScrFI TseI