Restriction map for
pFN21AB7993
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BpuAI BsaAI
BsaBI BsgI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI DraI
DraIII DrdI Eam1105I EarI Eco47III
EcoNI EcoRV EcoT22I FbaI FseI
FspI HindIII HinfI HpaI Ksp632I
MaeI MaeII MamI MfeI MluI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 845 GGTACC G^GTACC
AcsI 1248 GAATTC [GA]^AATT[CT]
AocI 672 CCTCAGG CC^T[ACGT]AGG
ApaI 168 GGGCCC GGGCC^C
ApoI 1248 GAATTC [GA]^AATT[CT]
Asp718I 845 GGTACC G^GTACC
AspHI 78 GAGCTC G[AT]GC[AT]^C
BalI 812 TGGCCA TGG^CCA
BglII 716 AGATCT A^GATCT
BlpI 38 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC
BsiEI 136 CGGCCG CG[GA][CT]^CG
BsiHKAI 78 GAGCTC G[AT]GC[AT]^C
BsmBI 688 GAGACG CGTCTC(1/5)
BspHI 1133 TCATGA T^CATGA
BspMI 465 GCAGGT ACCTGC(4/8)
BsrBI 83 CCGCTC CCGCTC(-3/-3)
BssSI 1049 CTCGTG CACGAG(-5/-1)
BstEII 195 GGTCACC G^GT[ACGT]ACC
BstPI 195 GGTCACC G^GT[ACGT]ACC
BstXI 429 CCAGATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 716 AGATCT [GA]^GATC[CT]
Bsu36I 672 CCTCAGG CC^T[ACGT]AGG
CelII 38 GCTCAGC GC^T[ACGT]AGC
CpoI 1372 CGGACCG CG^G[AT]CCG
EagI 136 CGGCCG C^GGCCG
EclXI 136 CGGCCG C^GGCCG
Eco52I 136 CGGCCG C^GGCCG
Eco81I 672 CCTCAGG CC^T[ACGT]AGG
EcoO65I 195 GGTCACC G^GT[ACGT]ACC
EcoRI 1248 GAATTC G^AATTC
HincII 696 GTTGAC GT[CT]^[GA]AC
HindII 696 GTTGAC GT[CT]^[GA]AC
KpnI 845 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
MaeIII 196 GTCAC ^GT[ACGT]AC
MflI 716 AGATCT [GA]^GATC[CT]
MluNI 812 TGGCCA TGG^CCA
MscI 812 TGGCCA TGG^CCA
NaeI 181 GCCGGC GCC^GGC
NgoMIV 181 GCCGGC G^CCGGC
NotI 135 GCGGCCGC GC^GGCCGC
NspI 503 GCATGC [GA]CATG^[CT]
PshAI 748 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1133 TCATGA T^CATGA
RsrII 1372 CGGACCG CG^G[AT]CCG
SacI 78 GAGCTC GAGCT^C
SacII 57 CCGCGG CCGC^GG
SphI 503 GCATGC GCATG^C
Tsp45I 196 GTCAC ^GT[GC]AC
XhoII 716 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 902 TCCGGA T^CCGGA
1370 TCCGGA
AvaI 51 CTCGGG C^[CT]CG[GA]G
1294 CTCGGG
BsaI 358 GAGACC GGTCTC(1/5)
862 GAGACC
BsaWI 902 TCCGGA [AT]^CCGG[AT]
1370 TCCGGA
BseAI 902 TCCGGA T^CCGGA
1370 TCCGGA
BsmFI 893 GGGAC GGGAC(10/14)
711 GTCCC
BsoBI 51 CTCGGG C^[CT]CG[GA]G
1294 CTCGGG
BspEI 902 TCCGGA T^CCGGA
1370 TCCGGA
BsrDI 1005 CATTGC GCAATG(2/0)
1313 GCAATG
BsrFI 181 GCCGGC [GA]^CCGG[CT]
1202 GCCGGT
BsrI 1255 ACTGG ACTGG(1/-1)
1385 CCAGT
Cfr10I 181 GCCGGC [GA]^CCGG[CT]
1202 GCCGGT
HgaI 235 GACGC GACGC(5/10)
369 GACGC
HphI 29 TCACC GGTGA(8/7)
197 TCACC
MboII 541 GAAGA GAAGA(8/7)
591 GAAGA
MroI 902 TCCGGA T^CCGGA
1370 TCCGGA
MseI 203 TTAA T^TAA
551 TTAA
MslI 726 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1134 CATGAGCGTG
NcoI 743 CCATGG C^CATGG
1304 CCATGG
PpuMI 676 AGGACCC [GA]G^G[AT]CC[CT]
1341 GGGTCCT
PvuII 563 CAGCTG CAG^CTG
1111 CAGCTG
SmlI 454 CTTGAG C^T[CT][GA]AG
1039 CTCAAG
Tru9I 203 TTAA T^TAA
551 TTAA
Tsp509I 549 AATT ^AATT
1249 AATT
XcmI 870 CCAGGCCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1091 CCAAGCAGGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TseI TspRI TthHB8I