(Circular) (Six-base) MAPSORT of: pFN21AB7993.seq Check: 2986 from: 1 to: 6112 pFN21AB7993 6112 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2461 2461 Size: 6112 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 2850 3433 2850 Size: 583 5529 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2615 2862 3380 4175 5559 5827 1632 Size: 370 613 247 518 795 1384 268 1917 Fragments arranged by size: 1917 1384 795 613 518 370 268 247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 2735 1166 Size: 1569 4543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2218 2573 2956 3855 5427 2218 Size: 355 383 899 1572 2903 Fragments arranged by size: 2903 1572 899 383 355 ApaI G_GGCC'C Cuts at: 2177 2177 Size: 6112 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3253 3421 3647 3799 5908 1493 Size: 1760 168 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2056 3299 3437 1870 Size: 73 113 1243 138 4545 Fragments arranged by size: 4545 1243 138 113 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2132 2183 2850 3433 Size: 325 217 627 279 66 51 667 583 Cuts at: 3433 4888 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 667 627 583 325 279 217 66 51 BanII G_rGCy'C Cuts at: 1391 2087 2177 3357 3431 1391 Size: 696 90 1180 74 4072 Fragments arranged by size: 4072 1180 696 90 74 BbeI G_GCGC'C Cuts at: 2070 2136 2187 2070 Size: 66 51 5995 Fragments arranged by size: 5995 66 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BbvCI CC'TCA_GC Cuts at: 2790 2835 3021 2790 Size: 45 186 5881 Fragments arranged by size: 5881 186 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 2124 2181 Size: 107 122 71 631 605 428 24 57 Cuts at: 2181 4723 136 Size: 2542 1525 Fragments arranged by size: 2542 1525 631 605 428 122 107 71 57 24 BglII A'GATC_T Cuts at: 2721 6107 2721 Size: 3386 2726 BlpI GC'TnA_GC Cuts at: 2044 3529 2044 Size: 1485 4627 Bme1580I G_kGCm'C Cuts at: 1790 2177 3406 4173 5329 1790 Size: 387 1229 767 1156 2573 Fragments arranged by size: 2573 1229 1156 767 387 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3843 4801 442 Size: 416 53 83 399 24 2426 958 1753 Fragments arranged by size: 2426 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2106 2106 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2360 2381 2510 2657 2858 3212 3237 Size: 1001 120 21 129 147 201 354 25 Cuts at: 3237 3319 4772 1239 Size: 82 1453 2579 Fragments arranged by size: 2579 1453 1001 354 201 147 129 120 82 25 21 Bpu10I CC'TnA_GC Cuts at: 2081 2790 2835 3021 3326 2081 Size: 709 45 186 305 4867 Fragments arranged by size: 4867 709 305 186 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 3029 4237 5102 5400 2480 Size: 549 1208 865 298 3192 Fragments arranged by size: 3192 1208 865 549 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 2861 4775 882 Size: 617 858 504 1914 2219 Fragments arranged by size: 2219 1914 858 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2133 2184 2241 Size: 53 83 186 936 534 66 51 57 Cuts at: 2241 3919 4301 4936 275 Size: 1678 382 635 1451 Fragments arranged by size: 1678 1451 936 635 534 382 186 83 66 57 53 51 BsaWI w'CCGG_w Cuts at: 2907 3375 4545 4943 5217 5364 5775 2907 Size: 468 1170 398 274 147 411 3244 Fragments arranged by size: 3244 1170 468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 2354 2378 2384 Size: 30 165 30 603 30 318 24 6 Cuts at: 2384 2408 2747 2777 3194 3210 3224 3240 1178 Size: 24 339 30 417 16 14 16 4050 Fragments arranged by size: 4050 603 417 339 318 165 30 30 30 30 24 24 16 16 14 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2825 3038 3083 3215 1186 Size: 1102 537 213 45 132 4083 Fragments arranged by size: 4083 1102 537 213 132 45 BseYI C'CCAG_C Cuts at: 2885 5315 5811 2885 Size: 2430 496 3186 Fragments arranged by size: 3186 2430 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2144 3562 4323 5351 1540 Size: 275 186 143 1418 761 1028 2301 Fragments arranged by size: 2301 1418 1028 761 275 186 143 BsiHKAI G_wGCw'C Cuts at: 2087 4173 4258 5329 2087 Size: 2086 85 1071 2870 Fragments arranged by size: 2870 2086 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 BsmBI CGTCTCn'nnnn_ Cuts at: 2687 2687 Size: 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2177 3357 3406 3431 4173 4258 Size: 399 297 90 1180 49 25 742 85 Cuts at: 4258 5329 1391 Size: 1071 2174 Fragments arranged by size: 2174 1180 1071 742 399 297 90 85 49 25 BspEI T'CCGG_A Cuts at: 2907 3375 5775 2907 Size: 468 2400 3244 Fragments arranged by size: 3244 2400 468 BspHI T'CATG_A Cuts at: 3138 4001 5731 3138 Size: 863 1730 3519 Fragments arranged by size: 3519 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2461 3467 844 Size: 1617 1006 3489 Fragments arranged by size: 3489 1617 1006 BsrBI CCG'CTC Cuts at: 1394 1823 2090 3999 1394 Size: 429 267 1909 3507 Fragments arranged by size: 3507 1909 429 267 BsrDI GCAATG_nn' Cuts at: 65 1248 3009 3325 4607 4781 65 Size: 1183 1761 316 1282 174 1396 Fragments arranged by size: 1761 1396 1282 1183 316 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 3207 4756 4943 1700 Size: 110 376 1021 1549 187 2869 Fragments arranged by size: 2869 1549 1021 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 3054 4172 5184 3054 Size: 1118 1012 3982 Fragments arranged by size: 3982 1118 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 2200 1184 Size: 1016 5096 BstXI CCAn_nnnn'nTGG Cuts at: 2441 2441 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2721 3442 3809 4194 4211 5663 5749 5761 Size: 749 721 367 385 17 1452 86 12 Cuts at: 5761 6107 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 749 721 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2678 2678 Size: 6112 BtgI C'CryG_G Cuts at: 513 1065 2062 2748 3309 513 Size: 552 997 686 561 3316 Fragments arranged by size: 3316 997 686 561 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2770 487 Size: 683 56 1544 3829 Fragments arranged by size: 3829 1544 683 56 BtsI GCAGTG_nn' Cuts at: 2465 3280 3346 3628 4422 4442 2465 Size: 815 66 282 794 20 4135 Fragments arranged by size: 4135 815 794 282 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 2151 2397 Size: 54 1425 50 275 18 311 10 246 Cuts at: 2397 2817 3559 4448 6077 8 Size: 420 742 889 1629 43 Fragments arranged by size: 1629 1425 889 742 420 311 275 246 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2141 3559 1537 Size: 275 329 1418 4090 Fragments arranged by size: 4090 1418 329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4042 1353 Size: 2689 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 3376 4699 5073 5219 387 Size: 1064 138 921 866 1323 374 146 1280 Fragments arranged by size: 1323 1280 1064 921 866 374 146 138 EcoICRI GAG'CTC Cuts at: 2085 2085 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2240 2360 2381 2510 2615 2657 Size: 393 370 238 120 21 129 105 42 Cuts at: 2657 2858 2862 3212 3237 3319 3380 4175 4772 Size: 201 4 350 25 82 61 795 597 Cuts at: 4772 5559 5827 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 597 393 370 350 268 238 201 129 120 105 82 61 42 25 21 4 EcoO109I rG'GnC_Cy Cuts at: 2173 2682 3347 2173 Size: 509 665 4938 Fragments arranged by size: 4938 665 509 EcoRI G'AATT_C Cuts at: 3253 3421 3799 3253 Size: 168 378 5566 Fragments arranged by size: 5566 378 168 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2070 2136 2187 3044 5259 1678 Size: 392 66 51 857 2215 2531 Fragments arranged by size: 2531 2215 857 392 66 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2699 2731 4753 Size: 32 135 32 806 32 289 32 2022 Cuts at: 4753 4785 4827 4859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2022 806 289 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 3456 3702 669 Size: 988 281 765 753 246 3079 Fragments arranged by size: 3079 988 765 753 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KasI G'GCGC_C Cuts at: 2066 2132 2183 2066 Size: 66 51 5995 Fragments arranged by size: 5995 66 51 KpnI G_GTAC'C Cuts at: 2854 3437 2854 Size: 583 5529 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2751 2979 5226 5410 1305 Size: 1446 228 2247 184 2007 Fragments arranged by size: 2247 2007 1446 228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 10 Size: 54 1425 343 987 3303 Fragments arranged by size: 3303 1425 987 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2735 3143 4070 4429 4588 518 Size: 648 1569 408 927 359 159 2042 Fragments arranged by size: 2042 1569 927 648 408 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2230 2260 2570 3091 3118 3528 4205 Size: 458 166 30 310 521 27 410 677 Cuts at: 4205 5353 5598 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 521 458 410 310 245 166 30 27 NaeI GCC'GGC Cuts at: 2188 2188 Size: 6112 NarI GG'CG_CC Cuts at: 2067 2133 2184 2067 Size: 66 51 5995 Fragments arranged by size: 5995 66 51 NcoI C'CATG_G Cuts at: 513 1065 2748 3309 513 Size: 552 1683 561 3316 Fragments arranged by size: 3316 1683 561 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NgoMIV G'CCGG_C Cuts at: 2186 2186 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 2141 3559 2141 Size: 1418 4694 NspI r_CATG'y Cuts at: 1132 1229 2512 3470 5015 1132 Size: 97 1283 958 1545 2229 Fragments arranged by size: 2229 1545 1283 958 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2682 3347 2682 Size: 665 5447 PshAI GACnn'nnGTC Cuts at: 1613 2757 1613 Size: 1144 4968 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PspOMI G'GGCC_C Cuts at: 2173 2173 Size: 6112 PstI C_TGCA'G Cuts at: 830 2385 2472 2715 2961 3090 3464 830 Size: 1555 87 243 246 129 374 3478 Fragments arranged by size: 3478 1555 374 246 243 129 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2570 3118 2570 Size: 548 5564 RsrII CG'GwC_CG Cuts at: 3378 3378 Size: 6112 SacI G_AGCT'C Cuts at: 2087 2087 Size: 6112 SacII CC_GC'GG Cuts at: 2065 2065 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2381 2468 2711 2957 3086 3460 4595 Size: 219 1336 87 243 246 129 374 1135 Cuts at: 4595 5276 5467 826 Size: 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 374 246 243 219 191 129 87 SfoI GGC'GCC Cuts at: 2068 2134 2185 2068 Size: 66 51 5995 Fragments arranged by size: 5995 66 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 3044 4216 5117 5379 820 Size: 197 926 516 585 1172 901 262 1553 Fragments arranged by size: 1553 1172 926 901 585 516 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 2512 3470 1132 Size: 1380 958 3774 Fragments arranged by size: 3774 1380 958 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2104 2643 2748 3309 3551 513 Size: 552 1039 539 105 561 242 3074 Fragments arranged by size: 3074 1039 561 552 539 242 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4139 4156 4309 4494 4939 325 Size: 1255 2559 17 153 185 445 1498 Fragments arranged by size: 2559 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 2354 2386 3210 3242 6072 6104 2006 Size: 32 316 32 824 32 2830 32 2014 Fragments arranged by size: 2830 2014 824 316 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2882 3103 2882 Size: 221 5891 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SanDI SapI SexAI SfiI SrfI SwaI