(Linear) (Six-base) MAPSORT of: KIBB7993.seq Check: 7950 from: 1 to: 1407 KIBB7993.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 456 1407 Size: 456 951 Acc65I G'GTAC_C Cuts at: 0 845 1407 Size: 845 562 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 610 857 1375 1407 Size: 610 247 518 32 Fragments arranged by size: 610 518 247 32 AleI CACnn'nnGTG Cuts at: 0 730 1407 Size: 730 677 AlwNI CAG_nnn'CTG Cuts at: 0 213 568 951 1407 Size: 213 355 383 456 Fragments arranged by size: 456 383 355 213 ApaI G_GGCC'C Cuts at: 0 172 1407 Size: 172 1235 ApoI r'AATT_y Cuts at: 0 1248 1407 Size: 1248 159 AvaI C'yCGr_G Cuts at: 0 51 1294 1407 Size: 51 1243 113 Fragments arranged by size: 1243 113 51 BanI G'GyrC_C Cuts at: 0 61 127 178 845 1407 Size: 61 66 51 667 562 Fragments arranged by size: 667 562 66 61 51 BanII G_rGCy'C Cuts at: 0 82 172 1352 1407 Size: 82 90 1180 55 Fragments arranged by size: 1180 90 82 55 BbeI G_GCGC'C Cuts at: 0 65 131 182 1407 Size: 65 66 51 1225 Fragments arranged by size: 1225 66 65 51 BbvCI CC'TCA_GC Cuts at: 0 785 830 1016 1407 Size: 785 45 186 391 Fragments arranged by size: 785 391 186 45 BglI GCCn_nnn'nGGC Cuts at: 0 95 119 176 1407 Size: 95 24 57 1231 Fragments arranged by size: 1231 95 57 24 BglII A'GATC_T Cuts at: 0 716 1407 Size: 716 691 BlpI GC'TnA_GC Cuts at: 0 39 1407 Size: 39 1368 Bme1580I G_kGCm'C Cuts at: 0 172 1401 1407 Size: 172 1229 6 Fragments arranged by size: 1229 172 6 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 101 1407 Size: 101 1306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 355 376 505 652 853 1207 1232 Size: 235 120 21 129 147 201 354 25 Cuts at: 1232 1314 1407 Size: 82 93 Fragments arranged by size: 354 235 201 147 129 120 93 82 25 21 Bpu10I CC'TnA_GC Cuts at: 0 76 785 830 1016 1321 1407 Size: 76 709 45 186 305 86 Fragments arranged by size: 709 305 186 86 76 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1024 1407 Size: 475 549 383 Fragments arranged by size: 549 475 383 BsaI GGTCTCn'nnnn_ Cuts at: 0 352 856 1407 Size: 352 504 551 Fragments arranged by size: 551 504 352 BsaHI Gr'CG_yC Cuts at: 0 62 128 179 236 1407 Size: 62 66 51 57 1171 Fragments arranged by size: 1171 66 62 57 51 BsaWI w'CCGG_w Cuts at: 0 902 1370 1407 Size: 902 468 37 Fragments arranged by size: 902 468 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1 31 349 373 379 403 742 772 Size: 1 30 318 24 6 24 339 30 Cuts at: 772 1189 1205 1219 1235 1407 Size: 417 16 14 16 172 Fragments arranged by size: 417 339 318 172 30 30 24 24 16 16 14 6 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 820 1033 1078 1210 1407 Size: 283 537 213 45 132 197 Fragments arranged by size: 537 283 213 197 132 45 BseYI C'CCAG_C Cuts at: 0 880 1407 Size: 880 527 BsiEI CG_ry'CG Cuts at: 0 139 1407 Size: 139 1268 BsiHKAI G_wGCw'C Cuts at: 0 82 1407 Size: 82 1325 BsmBI CGTCTCn'nnnn_ Cuts at: 0 682 1407 Size: 682 725 Bsp1286I G_dGCh'C Cuts at: 0 82 172 1352 1401 1407 Size: 82 90 1180 49 6 Fragments arranged by size: 1180 90 82 49 6 BspEI T'CCGG_A Cuts at: 0 902 1370 1407 Size: 902 468 37 Fragments arranged by size: 902 468 37 BspHI T'CATG_A Cuts at: 0 1133 1407 Size: 1133 274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 456 1407 Size: 456 951 BsrBI CCG'CTC Cuts at: 0 85 1407 Size: 85 1322 BsrDI GCAATG_nn' Cuts at: 0 1004 1320 1407 Size: 1004 316 87 Fragments arranged by size: 1004 316 87 BsrFI r'CCGG_y Cuts at: 0 181 1202 1407 Size: 181 1021 205 Fragments arranged by size: 1021 205 181 BssSI C'ACGA_G Cuts at: 0 1049 1407 Size: 1049 358 BstEII G'GTnAC_C Cuts at: 0 195 1407 Size: 195 1212 BstXI CCAn_nnnn'nTGG Cuts at: 0 436 1407 Size: 436 971 BstYI r'GATC_y Cuts at: 0 716 1407 Size: 716 691 Bsu36I CC'TnA_GG Cuts at: 0 673 1407 Size: 673 734 BtgI C'CryG_G Cuts at: 0 57 743 1304 1407 Size: 57 686 561 103 Fragments arranged by size: 686 561 103 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 765 1407 Size: 765 642 BtsI GCAGTG_nn' Cuts at: 0 460 1275 1341 1407 Size: 460 815 66 66 Fragments arranged by size: 815 460 66 66 EaeI y'GGCC_r Cuts at: 0 136 146 392 812 1407 Size: 136 10 246 420 595 Fragments arranged by size: 595 420 246 136 10 EagI C'GGCC_G Cuts at: 0 136 1407 Size: 136 1271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 505 1371 1407 Size: 505 866 36 Fragments arranged by size: 866 505 36 EcoICRI GAG'CTC Cuts at: 0 80 1407 Size: 80 1327 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 355 376 505 610 652 853 857 Size: 235 120 21 129 105 42 201 4 Cuts at: 857 1207 1232 1314 1375 1407 Size: 350 25 82 61 32 Fragments arranged by size: 350 235 201 129 120 105 82 61 42 32 25 21 4 EcoO109I rG'GnC_Cy Cuts at: 0 168 677 1342 1407 Size: 168 509 665 65 Fragments arranged by size: 665 509 168 65 EcoRI G'AATT_C Cuts at: 0 1248 1407 Size: 1248 159 HaeII r_GCGC'y Cuts at: 0 65 131 182 1039 1407 Size: 65 66 51 857 368 Fragments arranged by size: 857 368 66 65 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 373 405 694 726 1407 Size: 373 32 289 32 681 Fragments arranged by size: 681 373 289 32 32 HincII GTy'rAC Cuts at: 0 698 1407 Size: 698 709 KasI G'GCGC_C Cuts at: 0 61 127 178 1407 Size: 61 66 51 1229 Fragments arranged by size: 1229 66 61 51 KpnI G_GTAC'C Cuts at: 0 849 1407 Size: 849 558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 746 974 1407 Size: 746 228 433 Fragments arranged by size: 746 433 228 MscI TGG'CCA Cuts at: 0 814 1407 Size: 814 593 MslI CAynn'nnrTG Cuts at: 0 730 1138 1407 Size: 730 408 269 Fragments arranged by size: 730 408 269 MspA1I CmG'CkG Cuts at: 0 59 225 255 565 1086 1113 1407 Size: 59 166 30 310 521 27 294 Fragments arranged by size: 521 310 294 166 59 30 27 NaeI GCC'GGC Cuts at: 0 183 1407 Size: 183 1224 NarI GG'CG_CC Cuts at: 0 62 128 179 1407 Size: 62 66 51 1228 Fragments arranged by size: 1228 66 62 51 NcoI C'CATG_G Cuts at: 0 743 1304 1407 Size: 743 561 103 Fragments arranged by size: 743 561 103 NgoMIV G'CCGG_C Cuts at: 0 181 1407 Size: 181 1226 NotI GC'GGCC_GC Cuts at: 0 136 1407 Size: 136 1271 NspI r_CATG'y Cuts at: 0 507 1407 Size: 507 900 PpuMI rG'GwC_Cy Cuts at: 0 677 1342 1407 Size: 677 665 65 Fragments arranged by size: 677 665 65 PshAI GACnn'nnGTC Cuts at: 0 752 1407 Size: 752 655 PspOMI G'GGCC_C Cuts at: 0 168 1407 Size: 168 1239 PstI C_TGCA'G Cuts at: 0 380 467 710 956 1085 1407 Size: 380 87 243 246 129 322 Fragments arranged by size: 380 322 246 243 129 87 PvuII CAG'CTG Cuts at: 0 565 1113 1407 Size: 565 548 294 Fragments arranged by size: 565 548 294 RsrII CG'GwC_CG Cuts at: 0 1373 1407 Size: 1373 34 SacI G_AGCT'C Cuts at: 0 82 1407 Size: 82 1325 SacII CC_GC'GG Cuts at: 0 60 1407 Size: 60 1347 SfcI C'TryA_G Cuts at: 0 376 463 706 952 1081 1407 Size: 376 87 243 246 129 326 Fragments arranged by size: 376 326 246 243 129 87 SfoI GGC'GCC Cuts at: 0 63 129 180 1407 Size: 63 66 51 1227 Fragments arranged by size: 1227 66 63 51 SmlI C'TyrA_G Cuts at: 0 454 1039 1407 Size: 454 585 368 Fragments arranged by size: 585 454 368 SphI G_CATG'C Cuts at: 0 507 1407 Size: 507 900 StyI C'CwwG_G Cuts at: 0 99 638 743 1304 1407 Size: 99 539 105 561 103 Fragments arranged by size: 561 539 105 103 99 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1 33 349 381 1205 1237 1407 Size: 1 32 316 32 824 32 170 Fragments arranged by size: 824 316 170 32 32 32 1 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 877 1098 1407 Size: 877 221 309 Fragments arranged by size: 877 309 221 Enzymes that do cut: AarI Acc65I AcuI AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII SfcI SfoI SmlI SphI StyI TstI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BsaAI BsaBI BsgI BsiWI BsmI BsrGI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI