Restriction map for
pFN21AE4416
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccIII AciI AclI
AflII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AvrII
BalI BamHI BbeI BbrPI BbsI
BclI BcnI BfrI BlnI BpmI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI HincII HindII
HindIII HpaI HphI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 983 GGTACC G^GTACC
AccII 36 CGCG CG^CG
AcyI 114 GACGCC G[GA]^CG[CT]C
AflIII 557 ACATGT A^C[GA][CT]GT
AlwNI 1229 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 198 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 983 GGTACC G^GTACC
AviII 424 TGCGCA TGC^GCA
BanI 983 GGTACC G^G[CT][GA]CC
BanII 220 GAGCTC G[GA]GC[CT]^C
BciVI 448 GGATAC GTATCC(6/5)
BglI 853 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 492 AGATCT A^GATCT
BlpI 1037 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1037 GCTTAGC GC^T[ACGT]AGC
BsaHI 114 GACGCC G[GA]^CG[CT]C
BsaI 228 GGTCTC GGTCTC(1/5)
BseRI 1159 CTCCTC GAGGAG(10/8)
BsgI 133 CTGCAC GTGCAG(16/14)
BsmFI 71 GGGAC GGGAC(10/14)
BspLU11I 557 ACATGT A^CATGT
BsrFI 1064 ACCGGC [GA]^CCGG[CT]
BssHII 35 GCGCGC G^CGCGC
BstUI 36 CGCG CG^CG
BstXI 775 CCAGGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 492 AGATCT [GA]^GATC[CT]
CelII 1037 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1064 ACCGGC [GA]^CCGG[CT]
DpnII 493 GATC ^GATC
DraII 962 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 122 CCATGG C^C[GA][CT]GG
EcoNI 1027 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 962 AGGACCC [GA]G^G[ACGT]CC[CT]
FspI 424 TGCGCA TGC^GCA
HapII 1065 CCGG C^CGG
HgaI 114 GACGC GACGC(5/10)
Hin1I 114 GACGCC G[GA]^CG[CT]C
HpaII 1065 CCGG C^CGG
KpnI 983 GGTACC GGTAC^C
MaeII 944 ACGT A^CGT
MboI 493 GATC ^GATC
MflI 492 AGATCT [GA]^GATC[CT]
MslI 207 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1065 CCGG C^CGG
MvnI 36 CGCG CG^CG
NcoI 122 CCATGG C^CATGG
NdeII 493 GATC ^GATC
NheI 357 GCTAGC G^CTAGC
NspI 557 ACATGT [GA]CATG^[CT]
PflMI 122 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 962 AGGACCC [GA]G^G[AT]CC[CT]
SacI 220 GAGCTC GAGCT^C
Sau3AI 493 GATC ^GATC
ScaI 662 AGTACT AGT^ACT
SfcI 1049 CTGTAG C^T[GA][CT]AG
SmlI 914 CTCAAG C^T[CT][GA]AG
Van91I 122 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 492 AGATCT [GA]^GATC[CT]
XmnI 198 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Aor51HI 143 AGCGCT AGC^GCT
332 AGCGCT
AspHI 89 GAGCAC G[AT]GC[AT]^C
220 GAGCTC
BcgI 456 CGACATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
658 CGACAGTACTGC
BmyI 89 GAGCAC G[AGT]GC[ACT]^C
220 GAGCTC
BsiHKAI 89 GAGCAC G[AT]GC[AT]^C
220 GAGCTC
BsmAI 1001 GAGAC GTCTC(1/5)
229 GTCTC
Bsp1286I 89 GAGCAC G[AGT]GC[ACT]^C
220 GAGCTC
BspMI 421 ACCTGC ACCTGC(4/8)
250 GCAGGT
EarI 202 CTCTTC CTCTTC(1/4)
172 GAAGAG
Eco47III 143 AGCGCT AGC^GCT
332 AGCGCT
HaeII 143 AGCGCT [GA]GCGC^[CT]
332 AGCGCT
Ksp632I 202 CTCTTC CTCTTC(1/4)
172 GAAGAG
MboII 172 GAAGA GAAGA(8/7)
203 TCTTC
MspA1I 4 CAGCTG C[AC]G^C[GT]G
825 CAGCTG
PvuII 4 CAGCTG CAG^CTG
825 CAGCTG
SapI 172 GAAGAGC GCTCTTC(1/4)
201 GCTCTTC
Tsp45I 521 GTGAC ^GT[GC]AC
1214 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvCI BbvI BfaI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MnlI MseI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI