(Circular) (Six-base) MAPSORT of: pFN21AE4416.seq Check: 8485 from: 1 to: 5992 pFN21AE4416 5992 bp With 182 enzymes: SgfI * March 28, 2012 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 2988 3313 2988 Size: 325 5667 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 2411 2921 3233 4055 5439 5707 1632 Size: 674 105 510 312 822 1384 268 1917 Fragments arranged by size: 1917 1384 822 674 510 312 268 105 AfeI AGC'GCT Cuts at: 2150 2339 2150 Size: 189 5803 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2562 4891 5868 1203 Size: 1359 2329 977 1327 Fragments arranged by size: 2329 1359 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3260 1184 Size: 2076 3916 AlwNI CAG_nnn'CTG Cuts at: 3239 3735 5307 3239 Size: 496 1572 3924 Fragments arranged by size: 3924 1572 496 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 2507 2539 2686 3301 3527 3679 5788 1493 Size: 1014 32 147 615 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 615 226 152 147 32 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2988 3313 4768 618 Size: 325 217 627 1201 325 1455 1842 Fragments arranged by size: 1842 1455 1201 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 2229 3311 1391 Size: 838 1082 4072 Fragments arranged by size: 4072 1082 838 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5021 BbvCI CC'TCA_GC Cuts at: 2012 2334 2828 2012 Size: 322 494 5176 Fragments arranged by size: 5176 494 322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2450 2484 2652 2686 4183 Size: 34 50 34 429 34 168 34 1497 Cuts at: 4183 4217 1903 Size: 34 3678 Fragments arranged by size: 3678 1497 429 168 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2447 3884 5094 2447 Size: 1437 1210 3345 Fragments arranged by size: 3345 1437 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2864 4603 136 Size: 107 122 71 631 605 1192 1739 1525 Fragments arranged by size: 1739 1525 1192 631 605 122 107 71 BglII A'GATC_T Cuts at: 2497 5987 2497 Size: 3490 2502 BlpI GC'TnA_GC Cuts at: 3043 3409 3043 Size: 366 5626 Bme1580I G_kGCm'C Cuts at: 1790 4053 5209 1790 Size: 2263 1156 2573 Fragments arranged by size: 2573 2263 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 2497 3723 Size: 416 53 83 399 24 735 345 1226 Cuts at: 3723 4681 442 Size: 958 1753 Fragments arranged by size: 1753 1226 958 735 416 399 345 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2366 1056 Size: 1310 4682 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4652 1239 Size: 3413 2579 Bpu10I CC'TnA_GC Cuts at: 2012 2334 2828 2012 Size: 322 494 5176 Fragments arranged by size: 5176 494 322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2904 4117 4982 5280 2904 Size: 1213 865 298 3616 Fragments arranged by size: 3616 1213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2239 4655 882 Size: 617 740 2416 2219 Fragments arranged by size: 2416 2219 740 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2120 3799 4181 4816 Size: 53 83 186 936 587 1679 382 635 Cuts at: 4816 275 Size: 1451 Fragments arranged by size: 1679 1451 936 635 587 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3150 3180 1178 Size: 30 165 30 1747 30 3990 Fragments arranged by size: 3990 1747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3155 1186 Size: 1969 4023 BseYI C'CCAG_C Cuts at: 5195 5691 5195 Size: 496 5496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 5032 Fragments arranged by size: 5032 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2098 2229 4053 4138 5209 2098 Size: 131 1824 85 1071 2881 Fragments arranged by size: 2881 1824 1071 131 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2229 3311 4053 4138 5209 1391 Size: 399 308 131 1082 742 85 1071 2174 Fragments arranged by size: 2174 1082 1071 742 399 308 131 85 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2246 2435 3347 844 Size: 1402 189 912 3489 Fragments arranged by size: 3489 1402 912 189 BsrBI CCG'CTC Cuts at: 1394 1823 3879 1394 Size: 429 2056 3507 Fragments arranged by size: 3507 2056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3069 4636 4823 1700 Size: 110 1259 1567 187 2869 Fragments arranged by size: 2869 1567 1259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 2040 1925 Size: 115 5877 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2787 2787 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2497 3322 3689 4074 4091 5543 5629 5641 Size: 525 825 367 385 17 1452 86 12 Cuts at: 5641 5987 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 825 525 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2127 513 Size: 552 1062 4378 Fragments arranged by size: 4378 1062 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3088 487 Size: 683 56 1862 3391 Fragments arranged by size: 3391 1862 683 56 BtsI GCAGTG_nn' Cuts at: 3508 4302 4322 3508 Size: 794 20 5178 Fragments arranged by size: 5178 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957 Size: 54 1425 50 275 18 1609 889 1629 Cuts at: 5957 8 Size: 43 Fragments arranged by size: 1629 1609 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2213 3922 1353 Size: 819 41 1709 3423 Fragments arranged by size: 3423 1709 819 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 2227 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2411 2921 3233 4055 4652 5439 Size: 393 674 105 510 312 822 597 787 Cuts at: 5439 5707 1239 Size: 268 1524 Fragments arranged by size: 1524 822 787 674 597 510 393 312 268 105 EcoNI CCTnn'n_nnAGG Cuts at: 3036 3036 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 2968 2968 Size: 5992 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2336 2746 2951 3305 2336 Size: 410 205 354 5023 Fragments arranged by size: 5023 410 354 205 FspI TGC'GCA Cuts at: 2431 4498 2431 Size: 2067 3925 HaeII r_GCGC'y Cuts at: 1678 2152 2341 5139 1678 Size: 474 189 2798 2531 Fragments arranged by size: 2798 2531 474 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2726 2758 4633 4665 4707 Size: 32 135 32 1154 32 1875 32 42 Cuts at: 4707 4739 1373 Size: 32 2626 Fragments arranged by size: 2626 1875 1154 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 2992 3317 2992 Size: 325 5667 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2267 2395 2686 2768 2929 5106 5290 1305 Size: 962 128 291 82 161 2177 184 2007 Fragments arranged by size: 2177 2007 962 291 184 161 128 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 3950 4309 4468 518 Size: 648 1050 1734 359 159 2042 Fragments arranged by size: 2042 1734 1050 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2832 3408 4085 5233 5478 1606 Size: 405 821 576 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 677 576 405 245 NcoI C'CATG_G Cuts at: 513 1065 2127 513 Size: 552 1062 4378 Fragments arranged by size: 4378 1062 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 2362 1052 Size: 1310 4682 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2566 3350 4895 1132 Size: 97 1337 784 1545 2229 Fragments arranged by size: 2229 1545 1337 784 97 PasI CC'CwG_GG Cuts at: 1348 2084 2441 3118 1348 Size: 736 357 677 4222 Fragments arranged by size: 4222 736 677 357 PciI A'CATG_T Cuts at: 2562 4891 2562 Size: 2329 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 1108 Size: 1025 4967 PfoI T'CCnGG_A Cuts at: 2708 2708 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3018 3050 4061 4093 5574 5606 3018 Size: 32 1011 32 1481 32 3404 Fragments arranged by size: 3404 1481 1011 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2968 2968 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 2556 3562 2556 Size: 1006 4986 PstI C_TGCA'G Cuts at: 830 3344 830 Size: 2514 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2011 2832 2011 Size: 821 5171 SacI G_AGCT'C Cuts at: 2229 2229 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 2172 2213 2172 Size: 41 5951 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 2669 4240 5952 2669 Size: 1571 1712 2709 Fragments arranged by size: 2709 1712 1571 SfcI C'TryA_G Cuts at: 826 1045 3054 3340 4475 5156 5347 826 Size: 219 2009 286 1135 681 191 1471 Fragments arranged by size: 2009 1471 1135 681 286 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2919 4096 4997 5259 820 Size: 197 926 976 1177 901 262 1553 Fragments arranged by size: 1553 1177 976 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2127 2568 2973 3431 513 Size: 552 1062 441 405 458 3074 Fragments arranged by size: 3074 1062 552 458 441 405 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4019 4036 4189 4374 4819 325 Size: 1255 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 2667 4238 Size: 274 80 33 51 1443 624 66 1571 Cuts at: 4238 5950 96 Size: 1712 138 Fragments arranged by size: 1712 1571 1443 624 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3150 3182 5952 5984 3150 Size: 32 2770 32 3158 Fragments arranged by size: 3158 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 2207 4121 2207 Size: 1914 4078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI