(Linear) (Six-base) MAPSORT of: KIEE4416.seq Check: 6172 from: 1 to: 1287 KIEE4416.orf With 182 enzymes: SgfI * March 22, 2012 17:57 .. Acc65I G'GTAC_C Cuts at: 0 983 1287 Size: 983 304 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 406 916 1228 1287 Size: 301 105 510 312 59 Fragments arranged by size: 510 312 301 105 59 AfeI AGC'GCT Cuts at: 0 145 334 1287 Size: 145 189 953 Fragments arranged by size: 953 189 145 AflIII A'CryG_T Cuts at: 0 557 1287 Size: 557 730 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1255 1287 Size: 1255 32 AlwNI CAG_nnn'CTG Cuts at: 0 1234 1287 Size: 1234 53 ApoI r'AATT_y Cuts at: 0 502 534 681 1287 Size: 502 32 147 606 Fragments arranged by size: 606 502 147 32 BanI G'GyrC_C Cuts at: 0 983 1287 Size: 983 304 BanII G_rGCy'C Cuts at: 0 224 1287 Size: 224 1063 BbvCI CC'TCA_GC Cuts at: 0 7 329 823 1287 Size: 7 322 494 464 Fragments arranged by size: 494 464 322 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 445 479 647 681 1287 Size: 445 34 168 34 606 Fragments arranged by size: 606 445 168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 442 1287 Size: 442 845 BglI GCCn_nnn'nGGC Cuts at: 0 859 1287 Size: 859 428 BglII A'GATC_T Cuts at: 0 492 1287 Size: 492 795 BlpI GC'TnA_GC Cuts at: 0 1038 1287 Size: 1038 249 BmrI ACTGGGnnnn_n' Cuts at: 0 147 492 1287 Size: 147 345 795 Fragments arranged by size: 795 345 147 BmtI G_CTAG'C Cuts at: 0 361 1287 Size: 361 926 Bpu10I CC'TnA_GC Cuts at: 0 7 329 823 1287 Size: 7 322 494 464 Fragments arranged by size: 494 464 322 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 899 1287 Size: 899 388 BsaI GGTCTCn'nnnn_ Cuts at: 0 234 1287 Size: 234 1053 BsaHI Gr'CG_yC Cuts at: 0 115 1287 Size: 115 1172 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1145 1175 1287 Size: 1145 30 112 Fragments arranged by size: 1145 112 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1150 1287 Size: 1150 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 1287 Size: 118 1169 BsiHKAI G_wGCw'C Cuts at: 0 93 224 1287 Size: 93 131 1063 Fragments arranged by size: 1063 131 93 Bsp1286I G_dGCh'C Cuts at: 0 93 224 1287 Size: 93 131 1063 Fragments arranged by size: 1063 131 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 241 430 1287 Size: 241 189 857 Fragments arranged by size: 857 241 189 BsrFI r'CCGG_y Cuts at: 0 1064 1287 Size: 1064 223 BssHII G'CGCG_C Cuts at: 0 35 1287 Size: 35 1252 BstXI CCAn_nnnn'nTGG Cuts at: 0 782 1287 Size: 782 505 BstYI r'GATC_y Cuts at: 0 492 1287 Size: 492 795 BtgI C'CryG_G Cuts at: 0 122 1287 Size: 122 1165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1083 1287 Size: 1083 204 EarI CTCTTCn'nnn_ Cuts at: 0 167 208 1287 Size: 167 41 1079 Fragments arranged by size: 1079 167 41 EcoICRI GAG'CTC Cuts at: 0 222 1287 Size: 222 1065 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 406 916 1228 1287 Size: 301 105 510 312 59 Fragments arranged by size: 510 312 301 105 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 1031 1287 Size: 1031 256 EcoO109I rG'GnC_Cy Cuts at: 0 963 1287 Size: 963 324 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 331 741 946 1287 Size: 331 410 205 341 Fragments arranged by size: 410 341 331 205 FspI TGC'GCA Cuts at: 0 426 1287 Size: 426 861 HaeII r_GCGC'y Cuts at: 0 147 336 1287 Size: 147 189 951 Fragments arranged by size: 951 189 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 721 753 1287 Size: 721 32 534 Fragments arranged by size: 721 534 32 KpnI G_GTAC'C Cuts at: 0 987 1287 Size: 987 300 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 262 390 681 763 924 1287 Size: 262 128 291 82 161 363 Fragments arranged by size: 363 291 262 161 128 82 MslI CAynn'nnrTG Cuts at: 0 211 1287 Size: 211 1076 MspA1I CmG'CkG Cuts at: 0 6 827 1287 Size: 6 821 460 Fragments arranged by size: 821 460 6 NcoI C'CATG_G Cuts at: 0 122 1287 Size: 122 1165 NheI G'CTAG_C Cuts at: 0 357 1287 Size: 357 930 NspI r_CATG'y Cuts at: 0 561 1287 Size: 561 726 PasI CC'CwG_GG Cuts at: 0 79 436 1113 1287 Size: 79 357 677 174 Fragments arranged by size: 677 357 174 79 PciI A'CATG_T Cuts at: 0 557 1287 Size: 557 730 PflMI CCAn_nnn'nTGG Cuts at: 0 128 1287 Size: 128 1159 PfoI T'CCnGG_A Cuts at: 0 703 1287 Size: 703 584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1013 1045 1287 Size: 1013 32 242 Fragments arranged by size: 1013 242 32 PpuMI rG'GwC_Cy Cuts at: 0 963 1287 Size: 963 324 PsiI TTA'TAA Cuts at: 0 551 1287 Size: 551 736 PvuII CAG'CTG Cuts at: 0 6 827 1287 Size: 6 821 460 Fragments arranged by size: 821 460 6 SacI G_AGCT'C Cuts at: 0 224 1287 Size: 224 1063 SapI GCTCTTCn'nnn_ Cuts at: 0 167 208 1287 Size: 167 41 1079 Fragments arranged by size: 1079 167 41 ScaI AGT'ACT Cuts at: 0 664 1287 Size: 664 623 SfcI C'TryA_G Cuts at: 0 1049 1287 Size: 1049 238 SmlI C'TyrA_G Cuts at: 0 914 1287 Size: 914 373 StyI C'CwwG_G Cuts at: 0 122 563 968 1287 Size: 122 441 405 319 Fragments arranged by size: 441 405 319 122 TatI w'GTAC_w Cuts at: 0 596 662 1287 Size: 596 66 625 Fragments arranged by size: 625 596 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1145 1177 1287 Size: 1145 32 110 Fragments arranged by size: 1145 110 32 XmnI GAAnn'nnTTC Cuts at: 0 202 1287 Size: 202 1085 Enzymes that do cut: Acc65I AcuI AfeI AflIII AlfI AlwNI ApoI BanI BanII BbvCI BcgI BciVI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstXI BstYI BtgI BtgZI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KpnI MmeI MslI MspA1I NcoI NheI NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI PvuII SacI SapI ScaI SfcI SmlI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BclI BfrBI Bme1580I BmgBI BplI BpmI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI