################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI Ksp632I MamI MfeI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 436 GACGTC GACGT^C AcyI 436 GACGTC G[GA]^CG[CT]C AflIII 122 ACGCGT A^C[GA][CT]GT AhdI 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 57 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 295 AGCGCT AGC^GCT AspEI 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 75 CCCGAG C^[CT]CG[GA]G AvaII 197 GGTCC G^G[AT]CC BsaAI 112 TACGTG [CT]AC^GT[GA] BsaHI 436 GACGTC G[GA]^CG[CT]C BsgI 25 GTGCAG GTGCAG(16/14) BsoBI 75 CCCGAG C^[CT]CG[GA]G BspHI 416 TCATGA T^CATGA BsrBI 387 CCGCTC CCGCTC(-3/-3) BstAPI 56 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 96 GGTCACC G^GT[ACGT]ACC BstF5I 141 CATCC GGATG(2/0) BstPI 96 GGTCACC G^GT[ACGT]ACC CfoI 296 GCGC GCG^C DraII 196 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 103 CCGCGG C^C[GA][CT]GG EaeI 351 TGGCCG [CT]^GGCC[GA] Eam1105I 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 295 AGCGCT AGC^GCT EcoO109I 196 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 96 GGTCACC G^GT[ACGT]ACC EcoT14I 533 CCAAGG C^C[AT][AT]GG FauI 102 CCCGC CCCGC(4/6) FokI 141 CATCC GGATG(9/13) HaeII 295 AGCGCT [GA]GCGC^[CT] HgaI 124 GCGTC GACGC(5/10) HhaI 296 GCGC GCG^C Hin1I 436 GACGTC G[GA]^CG[CT]C HinP1I 296 GCGC G^CGC HincII 211 GTCAAC GT[CT]^[GA]AC HindII 211 GTCAAC GT[CT]^[GA]AC HinfI 238 GACTC G^A[ACGT]TC KspI 103 CCGCGG CCGC^GG MaeIII 97 GTCAC ^GT[ACGT]AC MboII 429 GAAGA GAAGA(8/7) MluI 122 ACGCGT A^CGCGT MslI 168 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 481 GGCCCC GG[ACGT]^[ACGT]CC PflMI 330 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 238 GACTC GAGTC(4/5) PpuMI 196 AGGTCCT [GA]G^G[AT]CC[CT] PstI 151 CTGCAG CTGCA^G RcaI 416 TCATGA T^CATGA SacI 16 GAGCTC GAGCT^C SacII 103 CCGCGG CCGC^GG StyI 533 CCAAGG C^C[AT][AT]GG TaqI 14 TCGA T^CGA Tsp45I 97 GTCAC ^GT[GC]AC TspRI 367 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 14 TCGA T^CGA Van91I 330 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwI 30 GGATC GGATC(4/5) 107 GGATC AspHI 16 GAGCTC G[AT]GC[AT]^C 358 GTGCTC BanII 6 GGGCTC G[GA]GC[CT]^C 16 GAGCTC BbvCI 291 CCTCAGC CCTCAGC(-5/-2) 187 GCTGAGG BcnI 354 CCGGG CC^[GC]GG 372 CCGGG BsiHKAI 16 GAGCTC G[AT]GC[AT]^C 358 GTGCTC BspMI 41 ACCTGC ACCTGC(4/8) 194 GCAGGT BstYI 30 GGATCT [GA]^GATC[CT] 107 GGATCT Cac8I 80 GCCAGC GC[ACGT]^[ACGT]GC 512 GCCAGC HphI 98 TCACC GGTGA(8/7) 167 TCACC MflI 30 GGATCT [GA]^GATC[CT] 107 GGATCT MspA1I 57 CAGCGG C[AC]G^C[GT]G 103 CCGCGG NciI 354 CCGGG CC^[GC]GG 372 CCGGG SfcI 66 CTACAG C^T[GA][CT]AG 151 CTGCAG XhoII 30 GGATCT [GA]^GATC[CT] 107 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AluI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeI MaeII MboI MnlI MspI MvaI MvnI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI TseI