Restriction map for
pFN21AE3663
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BbvI BcgI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I TseI Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 584 GTAC GT^AC
Alw44I 304 GTGCAC G^TGCAC
AocI 189 CCTAAGG CC^T[ACGT]AGG
ApaI 213 GGGCCC GGGCC^C
ApaLI 304 GTGCAC G^TGCAC
AspHI 304 GTGCAC G[AT]GC[AT]^C
AvaI 209 CCCGGG C^[CT]CG[GA]G
BalI 178 TGGCCA TGG^CCA
BamHI 447 GGATCC G^GATCC
BbsI 336 GAAGAC GAAGAC(2/6)
BciVI 349 GGATAC GTATCC(6/5)
BclI 491 TGATCA T^GATCA
BpuAI 336 GAAGAC GAAGAC(2/6)
BsaAI 352 TACGTG [CT]AC^GT[GA]
BsaI 53 GAGACC GGTCTC(1/5)
BsiHKAI 304 GTGCAC G[AT]GC[AT]^C
BsmBI 163 CGTCTC CGTCTC(1/5)
BsoBI 209 CCCGGG C^[CT]CG[GA]G
BsrI 149 ACTGG ACTGG(1/-1)
BstF5I 93 GGATG GGATG(2/0)
BstYI 447 GGATCC [GA]^GATC[CT]
Bsu36I 189 CCTAAGG CC^T[ACGT]AGG
CfoI 48 GCGC GCG^C
EaeI 178 TGGCCA [CT]^GGCC[GA]
EarI 376 GAAGAG CTCTTC(1/4)
Eco81I 189 CCTAAGG CC^T[ACGT]AGG
FbaI 491 TGATCA T^GATCA
Fnu4HI 41 GCCGC GC^[ACGT]GC
FokI 93 GGATG GGATG(9/13)
HhaI 48 GCGC GCG^C
HinP1I 48 GCGC G^CGC
HinfI 67 GACTC G^A[ACGT]TC
ItaI 41 GCCGC GC^[ACGT]GC
Ksp632I 376 GAAGAG CTCTTC(1/4)
MaeII 353 ACGT A^CGT
MflI 447 GGATCC [GA]^GATC[CT]
MluNI 178 TGGCCA TGG^CCA
MscI 178 TGGCCA TGG^CCA
MwoI 41 GCCGCCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 67 GACTC GAGTC(4/5)
PstI 437 CTGCAG CTGCA^G
RsaI 584 GTAC GT^AC
SfaNI 15 GATGC GCATC(5/9)
SfcI 437 CTGCAG C^T[GA][CT]AG
SmaI 209 CCCGGG CCC^GGG
TaqI 497 TCGA T^CGA
TspRI 219 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 497 TCGA T^CGA
XhoII 447 GGATCC [GA]^GATC[CT]
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 448 GATCC GGATC(4/5)
447 GGATC
BsmAI 53 GAGAC GTCTC(1/5)
164 GTCTC
BsmFI 65 GGGAC GGGAC(10/14)
511 GTCCC
DdeI 190 CTAAG C^T[ACGT]AG
407 CTAAG
DpnII 448 GATC ^GATC
492 GATC
HapII 63 CCGG C^CGG
210 CCGG
HpaII 63 CCGG C^CGG
210 CCGG
HphI 333 GGTGA GGTGA(8/7)
578 TCACC
MaeIII 454 GTGAC ^GT[ACGT]AC
577 GTCAC
MboI 448 GATC ^GATC
492 GATC
MboII 336 GAAGA GAAGA(8/7)
376 GAAGA
MspI 63 CCGG C^CGG
210 CCGG
NdeII 448 GATC ^GATC
492 GATC
PpuMI 256 AGGACCC [GA]G^G[AT]CC[CT]
509 AGGTCCC
Sau3AI 448 GATC ^GATC
492 GATC
Tsp45I 454 GTGAC ^GT[GC]AC
577 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI AvaII BanI BanII
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII HaeIII
MnlI MvaI NciI NlaIII NlaIV
Sau96I ScrFI