(Circular) (Six-base) MAPSORT of: pFN21AB6686.seq Check: 7748 from: 1 to: 6079 pFN21AB6686 6079 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 3422 1937 Size: 990 495 4594 Fragments arranged by size: 4594 990 495 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2557 4142 5526 5794 1632 Size: 815 110 1585 1384 268 1917 Fragments arranged by size: 1917 1585 1384 815 268 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 3191 4978 5955 1203 Size: 1988 1787 977 1327 Fragments arranged by size: 1988 1787 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 2750 3388 3614 3766 5875 1493 Size: 1257 638 226 152 2109 1697 Fragments arranged by size: 2109 1697 1257 638 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3230 3404 1870 Size: 73 1287 174 4545 Fragments arranged by size: 4545 1287 174 73 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 3400 4855 618 Size: 325 217 627 634 979 1455 1842 Fragments arranged by size: 1842 1455 979 634 627 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2438 2490 2658 2976 3135 3398 1391 Size: 636 411 52 168 318 159 263 4072 Fragments arranged by size: 4072 636 411 318 263 168 159 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BbvCI CC'TCA_GC Cuts at: 2880 3268 2880 Size: 388 5691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3211 3245 4270 4304 1903 Size: 34 1274 34 1025 34 3678 Fragments arranged by size: 3678 1274 1025 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2558 4690 136 Size: 107 122 71 631 605 886 2132 1525 Fragments arranged by size: 2132 1525 886 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3020 3125 3496 3020 Size: 105 371 5603 Fragments arranged by size: 5603 371 105 Bme1580I G_kGCm'C Cuts at: 1790 4140 5296 1790 Size: 2350 1156 2573 Fragments arranged by size: 2573 2350 1156 BmgBI CAC'GTC Cuts at: 1318 3194 1318 Size: 1876 4203 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2445 2714 1056 Size: 1389 269 4421 Fragments arranged by size: 4421 1389 269 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2399 2420 2558 2699 3017 3059 3218 Size: 1142 18 21 138 141 318 42 159 Cuts at: 3218 4739 1239 Size: 1521 2579 Fragments arranged by size: 2579 1521 1142 318 159 141 138 42 21 18 Bpu10I CC'TnA_GC Cuts at: 2448 2880 2975 3268 2448 Size: 432 95 293 5259 Fragments arranged by size: 5259 432 293 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4204 5069 5367 2564 Size: 1640 865 298 3276 Fragments arranged by size: 3276 1640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 2687 6073 2687 Size: 3386 2693 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3243 3886 4268 4903 Size: 53 83 186 936 1710 643 382 635 Cuts at: 4903 275 Size: 1451 Fragments arranged by size: 1710 1451 936 643 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2133 2163 1178 Size: 30 165 30 730 30 5094 Fragments arranged by size: 5094 730 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 2091 2291 2396 2753 1186 Size: 831 74 200 105 357 4512 Fragments arranged by size: 4512 831 357 200 105 74 BseYI C'CCAG_C Cuts at: 2071 2129 2437 3015 3120 5282 5778 2071 Size: 58 308 578 105 2162 496 2372 Fragments arranged by size: 2372 2162 578 496 308 105 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2925 1163 Size: 573 1189 4317 Fragments arranged by size: 4317 1189 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3529 4290 5318 1540 Size: 275 186 343 1185 761 1028 2301 Fragments arranged by size: 2301 1185 1028 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 2027 2490 2658 2976 3135 4140 4225 5296 2027 Size: 463 168 318 159 1005 85 1071 2810 Fragments arranged by size: 2810 1071 1005 463 318 168 159 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 BsmBI CGTCTCn'nnnn_ Cuts at: 3050 3050 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2438 2490 2658 2976 3135 3398 Size: 399 237 411 52 168 318 159 263 Cuts at: 3398 4140 4225 5296 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 318 263 237 168 159 85 52 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3434 844 Size: 2590 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2452 3966 1394 Size: 429 629 1514 3507 Fragments arranged by size: 3507 1514 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 4574 4748 65 Size: 1183 1069 2257 174 1396 Fragments arranged by size: 2257 1396 1183 1069 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 2371 1925 Size: 446 5633 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2409 2499 3090 3409 3776 4161 4178 5630 Size: 437 90 591 319 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 591 437 385 367 346 319 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2083 2853 513 Size: 552 1018 770 3739 Fragments arranged by size: 3739 1018 770 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2934 3145 3595 4389 4409 2934 Size: 211 450 794 20 4604 Fragments arranged by size: 4604 794 450 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2770 3526 4415 Size: 54 1425 50 275 18 940 756 889 Cuts at: 4415 6044 8 Size: 1629 43 Fragments arranged by size: 1629 1425 940 889 756 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 2700 2730 4009 1353 Size: 1125 222 30 1279 3423 Fragments arranged by size: 3423 1279 1125 222 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2003 4666 5040 5186 387 Size: 1064 138 414 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 414 374 146 138 EcoICRI GAG'CTC Cuts at: 2025 2488 2656 2974 3133 2025 Size: 463 168 318 159 4971 Fragments arranged by size: 4971 463 318 168 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2399 2420 2447 2557 2558 2699 Size: 393 749 18 21 27 110 1 141 Cuts at: 2699 3017 3059 3218 4142 4739 5526 5794 1239 Size: 318 42 159 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 749 597 393 318 268 159 141 110 42 27 21 18 1 EcoNI CCTnn'n_nnAGG Cuts at: 2363 2381 3230 2363 Size: 18 849 5212 Fragments arranged by size: 5212 849 18 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2996 3028 4720 4752 4794 Size: 32 135 32 1424 32 1692 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 1692 1424 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2779 3423 3669 669 Size: 988 281 337 504 644 246 3079 Fragments arranged by size: 3079 988 644 504 337 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5193 5377 1305 Size: 3888 184 2007 Fragments arranged by size: 3888 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4257 Fragments arranged by size: 4257 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 4037 4396 4555 518 Size: 648 1773 1098 359 159 2042 Fragments arranged by size: 2042 1773 1098 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2129 3019 3082 3124 3495 4172 5320 Size: 465 58 890 63 42 371 677 1148 Cuts at: 5320 5565 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 890 677 465 371 245 63 58 42 NcoI C'CATG_G Cuts at: 513 1065 2853 513 Size: 552 1788 3739 Fragments arranged by size: 3739 1788 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 2441 2710 1052 Size: 1389 269 4421 Fragments arranged by size: 4421 1389 269 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3437 4982 1132 Size: 97 2208 1545 2229 Fragments arranged by size: 2229 2208 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2614 2995 1108 Size: 1506 381 4192 Fragments arranged by size: 4192 1506 381 PfoI T'CCnGG_A Cuts at: 2490 2535 2490 Size: 45 6034 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2873 2905 4148 4180 5661 5693 2873 Size: 32 1243 32 1481 32 3259 Fragments arranged by size: 3259 1481 1243 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspXI vC'TCGA_Gb Cuts at: 3230 3230 Size: 6079 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3149 3181 3149 Size: 32 6047 PstI C_TGCA'G Cuts at: 830 2022 3012 3081 3087 3431 830 Size: 1192 990 69 6 344 3478 Fragments arranged by size: 3478 1192 990 344 69 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2071 2129 3019 3082 3124 2071 Size: 58 890 63 42 5026 Fragments arranged by size: 5026 890 63 58 42 SacI G_AGCT'C Cuts at: 2027 2490 2658 2976 3135 2027 Size: 463 168 318 159 4971 Fragments arranged by size: 4971 463 318 168 159 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2478 2700 2478 Size: 222 5857 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SexAI A'CCwGG_T Cuts at: 2760 2760 Size: 6079 SfcI C'TryA_G Cuts at: 826 1045 2018 2458 3008 3077 3083 3427 4562 Size: 219 973 440 550 69 6 344 1135 Cuts at: 4562 5243 5434 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 973 681 550 440 344 219 191 69 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 3230 4183 5084 5346 820 Size: 197 926 636 651 953 901 262 1553 Fragments arranged by size: 1553 953 926 901 651 636 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2994 3376 1517 Size: 1477 382 4220 Fragments arranged by size: 4220 1477 382 StyI C'CwwG_G Cuts at: 513 1065 2209 2233 2853 2989 3518 513 Size: 552 1144 24 620 136 529 3074 Fragments arranged by size: 3074 1144 620 552 529 136 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3165 4325 6037 Size: 274 80 33 51 1443 1188 1160 1712 Cuts at: 6037 96 Size: 138 Fragments arranged by size: 1712 1443 1188 1160 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 6039 6071 2133 Size: 32 3874 32 2141 Fragments arranged by size: 3874 2141 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2395 2560 2395 Size: 165 5914 XhoI C'TCGA_G Cuts at: 1943 3230 1943 Size: 1287 4792 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacII SanDI SfiI SfoI SrfI SwaI