(Circular) (Six-base) MAPSORT of: pFN21AE1530.seq Check: 938 from: 1 to: 6043 pFN21AE1530 6043 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 3386 1937 Size: 214 1235 4594 Fragments arranged by size: 4594 1235 214 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 2669 3107 4106 5490 5758 1632 Size: 856 181 438 999 1384 268 1917 Fragments arranged by size: 1917 1384 999 856 438 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2995 3133 3786 5358 2995 Size: 138 653 1572 3680 Fragments arranged by size: 3680 1572 653 138 ApaI G_GGCC'C Cuts at: 2854 2964 2854 Size: 110 5933 ApaLI G'TGCA_C Cuts at: 2018 2570 4100 5256 2018 Size: 552 1530 1156 2805 Fragments arranged by size: 2805 1530 1156 552 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2731 2864 3368 1870 Size: 73 788 133 504 4545 Fragments arranged by size: 4545 788 504 133 73 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2216 2776 3364 4819 Size: 325 217 627 267 162 560 588 1455 Cuts at: 4819 618 Size: 1842 Fragments arranged by size: 1842 1455 627 588 560 325 267 217 162 BanII G_rGCy'C Cuts at: 1391 2438 2550 2854 2871 2964 3362 1391 Size: 1047 112 304 17 93 398 4072 Fragments arranged by size: 4072 1047 398 304 112 93 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3007 3041 4234 4268 1903 Size: 34 1070 34 1193 34 3678 Fragments arranged by size: 3678 1193 1070 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3271 3935 5145 3271 Size: 664 1210 4169 Fragments arranged by size: 4169 1210 664 BclI T'GATC_A Cuts at: 1548 2073 1548 Size: 525 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2248 2397 4654 Size: 107 122 71 631 605 576 149 2257 Cuts at: 4654 136 Size: 1525 Fragments arranged by size: 2257 1525 631 605 576 149 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2022 2187 2319 2450 2574 2781 2854 2865 Size: 232 165 132 131 124 207 73 11 Cuts at: 2865 2964 4104 5260 1790 Size: 99 1140 1156 2573 Fragments arranged by size: 2573 1156 1140 232 207 165 132 131 124 99 73 11 BmgBI CAC'GTC Cuts at: 1318 2359 1318 Size: 1041 5002 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3774 4732 Size: 416 53 83 399 24 897 1460 958 Cuts at: 4732 442 Size: 1753 Fragments arranged by size: 1753 1460 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3175 3175 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4703 1239 Size: 917 2547 2579 Fragments arranged by size: 2579 2547 917 Bpu10I CC'TnA_GC Cuts at: 2437 2437 Size: 6043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4706 882 Size: 617 1092 2115 2219 Fragments arranged by size: 2219 2115 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 3834 493 Size: 711 909 1721 2702 Fragments arranged by size: 2702 1721 909 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2889 3850 4232 4867 Size: 53 83 186 936 1356 961 382 635 Cuts at: 4867 275 Size: 1451 Fragments arranged by size: 1451 1356 961 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4476 4874 5148 5295 5706 4476 Size: 398 274 147 411 4813 Fragments arranged by size: 4813 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 1178 Size: 30 165 30 694 30 5094 Fragments arranged by size: 5094 694 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3344 1186 Size: 1303 855 3885 Fragments arranged by size: 3885 1303 855 BseYI C'CCAG_C Cuts at: 2419 2874 5246 5742 2419 Size: 455 2372 496 2720 Fragments arranged by size: 2720 2372 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2313 2777 3173 1163 Size: 573 577 464 396 4033 Fragments arranged by size: 4033 577 573 464 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2103 3493 4254 5282 1540 Size: 275 186 102 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 102 BsiHKAI G_wGCw'C Cuts at: 2022 2088 2382 2574 2871 4104 4189 5260 2022 Size: 66 294 192 297 1233 85 1071 2805 Fragments arranged by size: 2805 1233 1071 297 294 192 85 66 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 BsmBI CGTCTCn'nnnn_ Cuts at: 2175 2175 Size: 6043 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2088 2187 2319 2382 2438 2450 Size: 399 232 66 99 132 63 56 12 Cuts at: 2450 2550 2574 2781 2854 2865 2871 2964 3362 Size: 100 24 207 73 11 6 93 398 Cuts at: 3362 4104 4189 5260 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 232 207 132 100 99 93 85 73 66 63 56 24 12 11 6 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 2493 3932 5662 2493 Size: 1439 1730 2874 Fragments arranged by size: 2874 1730 1439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3398 844 Size: 1916 638 3489 Fragments arranged by size: 3489 1916 638 BsrBI CCG'CTC Cuts at: 1394 1823 2821 3930 1394 Size: 429 998 1109 3507 Fragments arranged by size: 3507 1109 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 2710 1184 Size: 1526 4517 BstYI r'GATC_y Cuts at: 1972 2466 3373 3740 4125 4142 5594 5680 5692 Size: 494 907 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 494 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2220 2830 2923 2940 2956 3079 513 Size: 552 1155 610 93 17 16 123 3477 Fragments arranged by size: 3477 1155 610 552 123 93 17 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4436 Fragments arranged by size: 4436 868 683 56 BtsI GCAGTG_nn' Cuts at: 2355 2380 3559 4353 4373 2355 Size: 25 1179 794 20 4025 Fragments arranged by size: 4025 1179 794 25 20 ClaI AT'CG_AT Cuts at: 1554 3336 1554 Size: 1782 4261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DraIII CAC_nnn'GTG Cuts at: 2689 2689 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2281 2904 Size: 54 1425 50 275 18 270 181 623 Cuts at: 2904 2926 2943 2997 3057 3120 3490 4379 6008 Size: 22 17 54 60 63 370 889 1629 Cuts at: 6008 8 Size: 43 Fragments arranged by size: 1629 1425 889 623 370 275 270 181 63 60 54 54 50 43 22 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2100 3490 1537 Size: 275 288 1390 4090 Fragments arranged by size: 4090 1390 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3321 3973 1353 Size: 1968 652 3423 Fragments arranged by size: 3423 1968 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2683 3251 4630 5004 5150 387 Size: 1064 138 1094 568 1379 374 146 1280 Fragments arranged by size: 1379 1280 1094 1064 568 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2488 2669 3107 4106 4703 5490 Size: 393 524 332 181 438 999 597 787 Cuts at: 5490 5758 1239 Size: 268 1524 Fragments arranged by size: 1524 999 787 597 524 438 393 332 268 181 EcoO109I rG'GnC_Cy Cuts at: 2674 2851 2674 Size: 177 5866 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FspI TGC'GCA Cuts at: 2756 4549 2756 Size: 1793 4250 HaeII r_GCGC'y Cuts at: 1678 2129 2624 5190 1678 Size: 451 495 2566 2531 Fragments arranged by size: 2566 2531 495 451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 3245 3277 4684 Size: 32 135 32 581 32 1060 32 1407 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1407 1060 581 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 3309 748 Size: 2561 3482 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 5157 5341 1305 Size: 1206 2646 184 2007 Fragments arranged by size: 2646 2007 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 2945 2999 10 Size: 54 1425 343 451 662 54 3054 Fragments arranged by size: 3054 1425 662 451 343 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2969 4001 4360 4519 518 Size: 648 1374 429 1032 359 159 2042 Fragments arranged by size: 2042 1374 1032 648 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2578 2832 2958 3081 3459 4136 5284 Size: 528 444 254 126 123 378 677 1148 Cuts at: 5284 5529 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 528 444 378 254 245 126 123 NcoI C'CATG_G Cuts at: 513 1065 2220 2923 513 Size: 552 1155 703 3633 Fragments arranged by size: 3633 1155 703 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 2187 1348 Size: 839 5204 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2581 1108 Size: 956 517 4570 Fragments arranged by size: 4570 956 517 PfoI T'CCnGG_A Cuts at: 2481 3126 2481 Size: 645 5398 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2674 Size: 6043 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PspOMI G'GGCC_C Cuts at: 2850 2960 2850 Size: 110 5933 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 6011 PstI C_TGCA'G Cuts at: 830 2133 2397 3395 830 Size: 1303 264 998 3478 Fragments arranged by size: 3478 1303 998 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2134 2578 2134 Size: 444 5599 RsrII CG'GwC_CG Cuts at: 2485 2485 Size: 6043 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6043 SacII CC_GC'GG Cuts at: 2833 2959 3082 2833 Size: 126 123 5794 Fragments arranged by size: 5794 126 123 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SbfI CC_TGCA'GG Cuts at: 2397 3395 2397 Size: 998 5045 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SexAI A'CCwGG_T Cuts at: 2685 3255 2685 Size: 570 5473 SfcI C'TryA_G Cuts at: 826 1045 2129 2393 3391 4526 5207 5398 826 Size: 219 1084 264 998 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 998 681 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 2733 3370 1872 Size: 861 637 4545 Fragments arranged by size: 4545 861 637 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4378 StyI C'CwwG_G Cuts at: 513 1065 2220 2523 2923 3482 513 Size: 552 1155 303 400 559 3074 Fragments arranged by size: 3074 1155 559 552 400 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 4070 4087 4240 4425 4870 325 Size: 1255 1308 1182 17 153 185 445 1498 Fragments arranged by size: 1498 1308 1255 1182 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4289 6001 96 Size: 274 80 33 51 1443 2312 1712 138 Fragments arranged by size: 2312 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2097 2129 2675 2707 6003 6035 2097 Size: 32 546 32 3296 32 2105 Fragments arranged by size: 3296 2105 546 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2899 2977 2194 Size: 705 78 5260 Fragments arranged by size: 5260 705 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 2731 3368 1870 Size: 861 637 4545 Fragments arranged by size: 4545 861 637 XmnI GAAnn'nnTTC Cuts at: 2501 4172 2501 Size: 1671 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI SanDI SapI SfiI SfoI SrfI SwaI