################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AccI AccIII AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsgI BsiEI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 352 GGTACC G^GTACC AccII 1168 CGCG CG^CG Alw44I 240 GTGCAC G^TGCAC AocI 1047 CCTTAGG CC^T[ACGT]AGG ApaLI 240 GTGCAC G^TGCAC AseI 811 ATTAAT AT^TAAT AsnI 811 ATTAAT AT^TAAT Asp718I 352 GGTACC G^GTACC BbrPI 289 CACGTG CAC^GTG BbsI 1117 GAAGAC GAAGAC(2/6) BcgI 1016 GCATCGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1052 GGATAC GTATCC(6/5) BpuAI 1117 GAAGAC GAAGAC(2/6) BsaAI 289 CACGTG [CT]AC^GT[GA] BsaWI 488 TCCGGT [AT]^CCGG[AT] BsmBI 560 CGTCTC CGTCTC(1/5) BsmFI 146 GGGAC GGGAC(10/14) BsmI 49 GCATTC GAATGC(1/-1) BsrDI 665 GCAATG GCAATG(2/0) BstUI 1168 CGCG CG^CG Bsu36I 1047 CCTTAGG CC^T[ACGT]AGG DraI 1036 TTTAAA TTT^AAA DraII 721 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 874 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1171 GAAGAG CTCTTC(1/4) Eco81I 1047 CCTTAGG CC^T[ACGT]AGG EcoNI 410 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 721 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1138 GAATTC G^AATTC FauI 1068 CCCGC CCCGC(4/6) HaeIII 429 GGCC GG^CC HapII 489 CCGG C^CGG HgaI 690 GACGC GACGC(5/10) HincII 580 GTTAAC GT[CT]^[GA]AC HindII 580 GTTAAC GT[CT]^[GA]AC HpaI 580 GTTAAC GTT^AAC HpaII 489 CCGG C^CGG KpnI 352 GGTACC GGTAC^C Ksp632I 1171 GAAGAG CTCTTC(1/4) MslI 978 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 489 CCGG C^CGG MvnI 1168 CGCG CG^CG PflMI 1107 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 289 CACGTG CAC^GTG PmlI 289 CACGTG CAC^GTG PpuMI 721 AGGACCT [GA]G^G[AT]CC[CT] PshBI 811 ATTAAT AT^TAAT PstI 893 CTGCAG CTGCA^G PvuII 760 CAGCTG CAG^CTG SacI 918 GAGCTC GAGCT^C SfcI 893 CTGCAG C^T[GA][CT]AG SpeI 298 ACTAGT A^CTAGT Van91I 1107 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 421 GGTCC G^G[AT]CC 722 GGACC BanI 90 GGCACC G^G[CT][GA]CC 352 GGTACC BanII 859 GAGCCC G[GA]GC[CT]^C 918 GAGCTC BbvI 358 GCTGC GCAGC(8/12) 892 GCTGC BfaI 101 CTAG C^TAG 299 CTAG BpmI 123 CTCCAG CTGGAG(16/14) 924 CTGGAG BsiWI 675 CGTACG C^GTACG 702 CGTACG BsmAI 104 GTCTC GTCTC(1/5) 561 GTCTC Bsp1407I 455 TGTACA T^GTACA 974 TGTACA BsrGI 455 TGTACA T^GTACA 974 TGTACA BsrI 444 CCAGT ACTGG(1/-1) 1072 CCAGT BstF5I 606 CATCC GGATG(2/0) 529 GGATG BstYI 75 GGATCT [GA]^GATC[CT] 485 AGATCC FokI 606 CATCC GGATG(9/13) 529 GGATG MaeI 101 CTAG C^TAG 299 CTAG MflI 75 GGATCT [GA]^GATC[CT] 485 AGATCC MwoI 143 GCTGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1062 GCTGTTCCCGC SspBI 455 TGTACA T^GTACA 974 TGTACA TfiI 619 GATTC G^A[AT]TC 650 GATTC TseI 358 GCTGC G^C[AT]GC 892 GCTGC XhoII 75 GGATCT [GA]^GATC[CT] 485 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AatII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI Hin1I HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp45I Tsp509I TspRI