(Circular) (Six-base) MAPSORT of: pFN21AE1531.seq Check: 7865 from: 1 to: 5905 pFN21AE1531 5905 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2448 2775 2907 3715 Size: 53 83 186 936 912 327 132 808 Cuts at: 3715 278 Size: 2468 Fragments arranged by size: 2468 936 912 808 327 186 132 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 2357 3226 2357 Size: 869 5036 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 3968 5352 5620 1632 Size: 526 1810 1384 268 1917 Fragments arranged by size: 1917 1810 1384 526 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2329 2740 3067 3648 5220 2329 Size: 411 327 581 1572 3014 Fragments arranged by size: 3014 1572 581 411 327 ApaLI G'TGCA_C Cuts at: 2245 3962 5118 2245 Size: 1717 1156 3032 Fragments arranged by size: 3032 1717 1156 ApoI r'AATT_y Cuts at: 1493 2140 2259 2341 2371 2945 3143 3214 3440 Size: 647 119 82 30 574 198 71 226 Cuts at: 3440 3592 5701 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 647 574 226 198 152 119 82 71 30 AseI AT'TA_AT Cuts at: 160 1032 2817 4459 160 Size: 872 1785 1642 1606 Fragments arranged by size: 1785 1642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2357 3226 4681 618 Size: 325 217 627 308 262 869 1455 1842 Fragments arranged by size: 1842 1455 869 627 325 308 262 217 BanII G_rGCy'C Cuts at: 1391 2868 2927 3224 1391 Size: 1477 59 297 4072 Fragments arranged by size: 4072 1477 297 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3129 928 Size: 971 1230 3704 Fragments arranged by size: 3704 1230 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3010 3044 4096 4130 1903 Size: 34 50 34 989 34 1052 34 3678 Fragments arranged by size: 3678 1052 989 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3051 3797 5007 3051 Size: 746 1210 3949 Fragments arranged by size: 3949 1210 746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2249 3966 5122 1790 Size: 459 1717 1156 2573 Fragments arranged by size: 2573 1717 1156 459 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2113 2950 4565 1239 Size: 874 837 1615 2579 Fragments arranged by size: 2579 1615 874 837 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2296 3696 493 Size: 711 1092 1400 2702 Fragments arranged by size: 2702 1400 1092 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 2772 2904 3712 Size: 53 83 186 936 912 327 132 808 Cuts at: 3712 4094 4729 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 912 808 635 382 327 186 132 83 53 BsaWI w'CCGG_w Cuts at: 2493 4338 4736 5010 5157 5568 2493 Size: 1845 398 274 147 411 2830 Fragments arranged by size: 2830 1845 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2480 2510 1178 Size: 30 165 30 1077 30 4573 Fragments arranged by size: 4573 1077 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5905 BseYI C'CCAG_C Cuts at: 2050 2148 2666 2790 5108 5604 2050 Size: 98 518 124 2318 496 2351 Fragments arranged by size: 2351 2318 518 496 124 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2249 2927 2994 3966 4051 5122 2249 Size: 678 67 972 85 1071 3032 Fragments arranged by size: 3032 1071 972 678 85 67 BsiWI C'GTAC_G Cuts at: 2680 2707 2680 Size: 27 5878 BsmI GAATG_Cn' Cuts at: 2054 3416 3509 2054 Size: 1362 93 4450 Fragments arranged by size: 4450 1362 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2571 2571 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2249 2868 2927 2994 3224 3966 4051 Size: 399 459 619 59 67 230 742 85 Cuts at: 4051 5122 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 619 459 399 230 85 67 59 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2677 4400 4574 65 Size: 1183 1429 1723 174 1396 Fragments arranged by size: 1723 1429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 2460 2979 96 Size: 2364 519 3022 Fragments arranged by size: 3022 2364 519 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2080 2490 3235 3602 3987 4004 5456 5542 Size: 108 410 745 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 745 410 385 367 346 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3053 3053 Size: 5905 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 2698 3421 4215 4235 2698 Size: 723 794 20 4368 Fragments arranged by size: 4368 794 723 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3043 3209 3043 Size: 166 5739 DraIII CAC_nnn'GTG Cuts at: 2884 2884 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870 Size: 54 1425 50 275 18 1522 889 1629 Cuts at: 5870 8 Size: 43 Fragments arranged by size: 1629 1522 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3171 3835 1353 Size: 1818 664 3423 Fragments arranged by size: 3423 1818 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2925 2925 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2158 2950 3968 4565 5352 5620 Size: 393 481 45 792 1018 597 787 268 Cuts at: 5620 1239 Size: 1524 Fragments arranged by size: 1524 1018 792 787 597 481 393 268 45 EcoNI CCTnn'n_nnAGG Cuts at: 2419 2419 Size: 5905 EcoO109I rG'GnC_Cy Cuts at: 2727 2727 Size: 5905 EcoRI G'AATT_C Cuts at: 3143 3214 3592 3143 Size: 71 378 5456 Fragments arranged by size: 5456 378 71 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 2537 2569 4546 Size: 32 135 32 910 32 23 32 1977 Cuts at: 4546 4578 4620 4652 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1977 910 135 42 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2587 3249 3495 669 Size: 988 281 649 662 246 3079 Fragments arranged by size: 3079 988 662 649 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 2587 3495 2587 Size: 908 4997 KpnI G_GTAC'C Cuts at: 2361 3230 2361 Size: 869 5036 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2453 5019 5203 1305 Size: 1148 2566 184 2007 Fragments arranged by size: 2566 2007 1148 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4083 Fragments arranged by size: 4083 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2987 3863 4222 4381 518 Size: 648 1821 876 359 159 2042 Fragments arranged by size: 2042 1821 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2363 2767 3064 3321 3998 5146 5391 1606 Size: 757 404 297 257 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 677 404 297 257 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 1108 Size: 2010 3895 PfoI T'CCnGG_A Cuts at: 2129 2927 2129 Size: 798 5107 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PmlI CAC'GTG Cuts at: 2296 2296 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2208 2240 2957 2989 3974 4006 5487 5519 2208 Size: 32 717 32 985 32 1481 32 2594 Fragments arranged by size: 2594 1481 985 717 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2727 2727 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PstI C_TGCA'G Cuts at: 830 2902 3257 830 Size: 2072 355 3478 Fragments arranged by size: 3478 2072 355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2767 2767 Size: 5905 SacI G_AGCT'C Cuts at: 2927 2927 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2898 3253 4388 5069 5260 826 Size: 219 1853 355 1135 681 191 1471 Fragments arranged by size: 1853 1471 1135 681 355 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 2303 152 Size: 2151 3754 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 3344 513 Size: 552 2279 3074 Fragments arranged by size: 3074 2279 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 3932 3949 4102 4287 4732 325 Size: 1255 484 1868 17 153 185 445 1498 Fragments arranged by size: 1868 1498 1255 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 2979 4151 Size: 274 80 33 51 1443 483 519 1172 Cuts at: 4151 5863 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 519 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2514 2546 5865 5897 2480 Size: 32 2 32 3319 32 2488 Fragments arranged by size: 3319 2488 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2446 2773 2905 3713 Size: 53 83 186 936 912 327 132 808 Cuts at: 3713 276 Size: 2468 Fragments arranged by size: 2468 936 912 808 327 186 132 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI