(Linear) (Six-base) MAPSORT of: KIEE1531.seq Check: 2724 from: 1 to: 1200 KIEE1531.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AatII G_ACGT'C Cuts at: 0 443 770 902 1200 Size: 443 327 132 298 Fragments arranged by size: 443 327 298 132 Acc65I G'GTAC_C Cuts at: 0 352 1200 Size: 352 848 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 1200 Size: 153 1047 AlwNI CAG_nnn'CTG Cuts at: 0 324 735 1062 1200 Size: 324 411 327 138 Fragments arranged by size: 411 327 324 138 ApaLI G'TGCA_C Cuts at: 0 240 1200 Size: 240 960 ApoI r'AATT_y Cuts at: 0 135 254 336 366 940 1138 1200 Size: 135 119 82 30 574 198 62 Fragments arranged by size: 574 198 135 119 82 62 30 AseI AT'TA_AT Cuts at: 0 812 1200 Size: 812 388 BanI G'GyrC_C Cuts at: 0 90 352 1200 Size: 90 262 848 Fragments arranged by size: 848 262 90 BanII G_rGCy'C Cuts at: 0 863 922 1200 Size: 863 59 278 Fragments arranged by size: 863 278 59 BbsI GAAGACnn'nnnn_ Cuts at: 0 1124 1200 Size: 1124 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1005 1039 1200 Size: 1005 34 161 Fragments arranged by size: 1005 161 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1046 1200 Size: 1046 154 Bme1580I G_kGCm'C Cuts at: 0 244 1200 Size: 244 956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 945 1200 Size: 108 837 255 Fragments arranged by size: 837 255 108 BsaAI yAC'GTr Cuts at: 0 291 1200 Size: 291 909 BsaHI Gr'CG_yC Cuts at: 0 440 767 899 1200 Size: 440 327 132 301 Fragments arranged by size: 440 327 301 132 BsaWI w'CCGG_w Cuts at: 0 488 1200 Size: 488 712 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 475 505 1200 Size: 475 30 695 Fragments arranged by size: 695 475 30 BseYI C'CCAG_C Cuts at: 0 45 143 661 785 1200 Size: 45 98 518 124 415 Fragments arranged by size: 518 415 124 98 45 BsiHKAI G_wGCw'C Cuts at: 0 244 922 989 1200 Size: 244 678 67 211 Fragments arranged by size: 678 244 211 67 BsiWI C'GTAC_G Cuts at: 0 675 702 1200 Size: 675 27 498 Fragments arranged by size: 675 498 27 BsmI GAATG_Cn' Cuts at: 0 49 1200 Size: 49 1151 BsmBI CGTCTCn'nnnn_ Cuts at: 0 566 1200 Size: 566 634 Bsp1286I G_dGCh'C Cuts at: 0 244 863 922 989 1200 Size: 244 619 59 67 211 Fragments arranged by size: 619 244 211 67 59 BsrDI GCAATG_nn' Cuts at: 0 672 1200 Size: 672 528 BsrGI T'GTAC_A Cuts at: 0 455 974 1200 Size: 455 519 226 Fragments arranged by size: 519 455 226 BstYI r'GATC_y Cuts at: 0 75 485 1200 Size: 75 410 715 Fragments arranged by size: 715 410 75 Bsu36I CC'TnA_GG Cuts at: 0 1048 1200 Size: 1048 152 BtsI GCAGTG_nn' Cuts at: 0 693 1200 Size: 693 507 DraI TTT'AAA Cuts at: 0 1038 1200 Size: 1038 162 DraIII CAC_nnn'GTG Cuts at: 0 879 1200 Size: 879 321 EarI CTCTTCn'nnn_ Cuts at: 0 1166 1200 Size: 1166 34 EcoICRI GAG'CTC Cuts at: 0 920 1200 Size: 920 280 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 153 945 1200 Size: 108 45 792 255 Fragments arranged by size: 792 255 108 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 414 1200 Size: 414 786 EcoO109I rG'GnC_Cy Cuts at: 0 722 1200 Size: 722 478 EcoRI G'AATT_C Cuts at: 0 1138 1200 Size: 1138 62 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 198 1200 Size: 198 1002 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 477 509 532 564 1200 Size: 477 32 23 32 636 Fragments arranged by size: 636 477 32 32 23 HincII GTy'rAC Cuts at: 0 582 1200 Size: 582 618 HpaI GTT'AAC Cuts at: 0 582 1200 Size: 582 618 KpnI G_GTAC'C Cuts at: 0 356 1200 Size: 356 844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 448 1200 Size: 448 752 MslI CAynn'nnrTG Cuts at: 0 982 1200 Size: 982 218 MspA1I CmG'CkG Cuts at: 0 358 762 1059 1200 Size: 358 404 297 141 Fragments arranged by size: 404 358 297 141 PflMI CCAn_nnn'nTGG Cuts at: 0 1113 1200 Size: 1113 87 PfoI T'CCnGG_A Cuts at: 0 124 922 1200 Size: 124 798 278 Fragments arranged by size: 798 278 124 PmlI CAC'GTG Cuts at: 0 291 1200 Size: 291 909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 203 235 952 984 1200 Size: 203 32 717 32 216 Fragments arranged by size: 717 216 203 32 32 PpuMI rG'GwC_Cy Cuts at: 0 722 1200 Size: 722 478 PstI C_TGCA'G Cuts at: 0 897 1200 Size: 897 303 PvuII CAG'CTG Cuts at: 0 762 1200 Size: 762 438 SacI G_AGCT'C Cuts at: 0 922 1200 Size: 922 278 SfcI C'TryA_G Cuts at: 0 893 1200 Size: 893 307 SpeI A'CTAG_T Cuts at: 0 298 1200 Size: 298 902 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 59 1200 Size: 59 1141 TatI w'GTAC_w Cuts at: 0 455 974 1200 Size: 455 519 226 Fragments arranged by size: 519 455 226 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 475 507 509 541 1200 Size: 475 32 2 32 659 Fragments arranged by size: 659 475 32 32 2 ZraI GAC'GTC Cuts at: 0 441 768 900 1200 Size: 441 327 132 300 Fragments arranged by size: 441 327 300 132 Enzymes that do cut: AatII Acc65I AcuI AlwNI ApaLI ApoI AseI BanI BanII BbsI BcgI BciVI Bme1580I BpmI BsaAI BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BsrDI BsrGI BstYI Bsu36I BtsI DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HpaI KpnI MmeI MslI MspA1I PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SfcI SpeI TaqII TatI TstI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BseRI BsgI BsiEI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DrdI EaeI EagI EciI EcoRV FseI FspI FspAI HaeII HindIII KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI