(Circular) (Six-base) MAPSORT of: pFN21AE4477.seq Check: 6841 from: 1 to: 6190 pFN21AE4477 6190 bp With 182 enzymes: SgfI * March 28, 2012 14:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 3533 1937 Size: 268 1328 4594 Fragments arranged by size: 4594 1328 268 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4253 5637 5905 1632 Size: 2621 1384 268 1917 Fragments arranged by size: 2621 1917 1384 268 AfeI AGC'GCT Cuts at: 2939 2939 Size: 6190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 3294 5089 6066 1203 Size: 2091 1795 977 1327 Fragments arranged by size: 2091 1795 1327 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3257 1184 Size: 2073 4117 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3404 3436 3404 Size: 32 6158 AlwNI CAG_nnn'CTG Cuts at: 2710 3349 3933 5505 2710 Size: 639 584 1572 3395 Fragments arranged by size: 3395 1572 639 584 ApaI G_GGCC'C Cuts at: 2113 2121 3429 2113 Size: 8 1308 4874 Fragments arranged by size: 4874 1308 8 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 2101 3499 3725 3877 5986 1493 Size: 608 1398 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 608 226 152 AscI GG'CGCG_CC Cuts at: 2732 2732 Size: 6190 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2112 2195 3029 3329 3428 3515 Size: 73 163 6 83 834 300 99 87 Cuts at: 3515 1870 Size: 4545 Fragments arranged by size: 4545 834 300 163 99 87 83 73 6 AvrII C'CTAG_G Cuts at: 2096 2096 Size: 6190 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2413 2505 2638 2660 Size: 325 217 627 404 222 92 133 22 Cuts at: 2660 3352 3385 3511 4966 618 Size: 692 33 126 1455 1842 Fragments arranged by size: 1842 1455 692 627 404 325 222 217 133 126 92 33 22 BanII G_rGCy'C Cuts at: 1391 2113 2121 3417 3429 3509 1391 Size: 722 8 1296 12 80 4072 Fragments arranged by size: 4072 1296 722 80 12 8 BbeI G_GCGC'C Cuts at: 2642 3389 2642 Size: 747 5443 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5219 BbvCI CC'TCA_GC Cuts at: 2409 2409 Size: 6190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4381 4415 1903 Size: 34 50 34 2360 34 3678 Fragments arranged by size: 3678 2360 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 4801 136 Size: 107 122 71 631 605 364 2765 1525 Fragments arranged by size: 2765 1525 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 2775 3607 2775 Size: 832 5358 Bme1580I G_kGCm'C Cuts at: 1790 2113 2121 2196 2663 3357 3429 4251 5407 Size: 323 8 75 467 694 72 822 1156 Cuts at: 5407 1790 Size: 2573 Fragments arranged by size: 2573 1156 822 694 467 323 75 72 8 BmgBI CAC'GTC Cuts at: 1318 2465 3091 3118 1318 Size: 1147 626 27 4390 Fragments arranged by size: 4390 1147 626 27 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3442 3921 4879 Size: 416 53 83 399 24 2025 479 958 Cuts at: 4879 442 Size: 1753 Fragments arranged by size: 2025 1753 958 479 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3172 3055 Size: 117 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2447 2876 3023 3431 4850 1239 Size: 1061 147 429 147 408 1419 2579 Fragments arranged by size: 2579 1419 1061 429 408 147 147 Bpu10I CC'TnA_GC Cuts at: 2409 2409 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3401 4315 5180 5478 3401 Size: 914 865 298 4113 Fragments arranged by size: 4113 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 2873 2969 4853 882 Size: 617 852 522 96 1884 2219 Fragments arranged by size: 2219 1884 852 617 522 96 BsaAI yAC'GTr Cuts at: 493 1204 2365 3247 3297 3981 493 Size: 711 1161 882 50 684 2702 Fragments arranged by size: 2702 1161 882 711 684 50 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2580 2639 2945 3279 Size: 53 83 186 936 1047 59 306 334 Cuts at: 3279 3386 3997 4379 5014 275 Size: 107 611 382 635 1451 Fragments arranged by size: 1451 1047 936 635 611 382 334 306 186 107 83 59 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 3404 3434 1178 Size: 30 165 30 1180 30 791 30 3934 Fragments arranged by size: 3934 1180 791 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2948 1186 Size: 1762 4428 BseYI C'CCAG_C Cuts at: 2227 2232 2694 2818 2838 3046 5393 5889 2227 Size: 5 462 124 20 208 2347 496 2528 Fragments arranged by size: 2528 2347 496 462 208 124 20 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2723 3239 1163 Size: 573 987 516 4114 Fragments arranged by size: 4114 987 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2654 3198 3640 4401 5429 Size: 275 186 39 614 544 442 761 1028 Cuts at: 5429 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 614 544 442 275 186 39 BsiHKAI G_wGCw'C Cuts at: 2607 3336 3417 4251 4336 5407 2607 Size: 729 81 834 85 1071 3390 Fragments arranged by size: 3390 1071 834 729 85 81 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2121 2196 2607 2663 3336 3357 Size: 399 323 8 75 411 56 673 21 Cuts at: 3357 3417 3429 3509 4251 4336 5407 1391 Size: 60 12 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 411 399 323 85 80 75 60 56 21 12 8 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 3282 4079 5809 3282 Size: 797 1730 3663 Fragments arranged by size: 3663 1730 797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3545 844 Size: 2701 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3280 4685 4859 65 Size: 1183 2032 1405 174 1396 Fragments arranged by size: 2032 1405 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2331 2653 3152 3309 3368 4834 Size: 110 328 193 322 499 157 59 1466 Cuts at: 4834 5021 1700 Size: 187 2869 Fragments arranged by size: 2869 1466 499 328 322 193 187 157 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 2611 2732 2762 2814 2962 2964 1925 Size: 686 121 30 52 148 2 5151 Fragments arranged by size: 5151 686 148 121 52 30 2 BssSI C'ACGA_G Cuts at: 2077 4250 5262 2077 Size: 2173 1012 3005 Fragments arranged by size: 3005 2173 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3340 794 Size: 2546 3644 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 6190 BstYI r'GATC_y Cuts at: 1972 3520 3887 4272 4289 5741 5827 5839 6185 Size: 1548 367 385 17 1452 86 12 346 Cuts at: 6185 1972 Size: 1977 Fragments arranged by size: 1977 1548 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2238 2487 2727 2751 2901 3061 3077 Size: 552 1173 249 240 24 150 160 16 Cuts at: 3077 513 Size: 3626 Fragments arranged by size: 3626 1173 552 249 240 160 150 24 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 3041 3464 3706 4500 4520 3041 Size: 423 242 794 20 4711 Fragments arranged by size: 4711 794 423 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2359 2394 529 Size: 35 1795 35 4325 Fragments arranged by size: 4325 1795 35 35 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2333 2367 Size: 54 1425 50 275 18 207 296 34 Cuts at: 2367 2472 2490 2651 2748 3637 4526 6155 8 Size: 105 18 161 97 889 889 1629 43 Fragments arranged by size: 1629 1425 889 889 296 275 207 161 105 97 54 50 43 34 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 2651 3637 1537 Size: 275 225 614 986 4090 Fragments arranged by size: 4090 986 614 275 225 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2630 4120 1353 Size: 719 558 1490 3423 Fragments arranged by size: 3423 1490 719 558 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2816 4777 5151 5297 387 Size: 1064 138 1227 1961 374 146 1280 Fragments arranged by size: 1961 1280 1227 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3415 3415 Size: 6190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2447 2876 3023 3431 4253 4850 Size: 393 668 147 429 147 408 822 597 Cuts at: 4850 5637 5905 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 787 668 597 429 408 393 268 147 147 EcoNI CCTnn'n_nnAGG Cuts at: 2930 2930 Size: 6190 EcoO109I rG'GnC_Cy Cuts at: 2117 2118 2117 Size: 1 6189 EcoRI G'AATT_C Cuts at: 2101 3499 3877 2101 Size: 1398 378 4414 Fragments arranged by size: 4414 1398 378 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 2312 2447 2642 2807 2941 3389 5337 1678 Size: 634 135 195 165 134 448 1948 2531 Fragments arranged by size: 2531 1948 634 448 195 165 135 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4831 4863 4905 4937 1373 Size: 32 135 32 3259 32 42 32 2626 Fragments arranged by size: 3259 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KasI G'GCGC_C Cuts at: 2638 3385 2638 Size: 747 5443 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5304 5488 1305 Size: 3999 184 2007 Fragments arranged by size: 3999 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2750 10 Size: 54 1425 343 537 381 3450 Fragments arranged by size: 3450 1425 537 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4148 4507 4666 518 Size: 648 2982 359 159 2042 Fragments arranged by size: 2982 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 2574 2707 2729 2746 3136 3349 3606 Size: 413 555 133 22 17 390 213 257 Cuts at: 3606 4283 5431 5676 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 555 413 390 257 245 213 133 22 17 NaeI GCC'GGC Cuts at: 2140 3154 3370 2140 Size: 1014 216 4960 Fragments arranged by size: 4960 1014 216 NarI GG'CG_CC Cuts at: 2639 3386 2639 Size: 747 5443 NcoI C'CATG_G Cuts at: 513 1065 3077 513 Size: 552 2012 3626 Fragments arranged by size: 3626 2012 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NgoMIV G'CCGG_C Cuts at: 2138 3152 3368 2138 Size: 1014 216 4960 Fragments arranged by size: 4960 1014 216 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 2037 3637 2037 Size: 1600 4590 NspI r_CATG'y Cuts at: 1132 1229 3548 5093 1132 Size: 97 2319 1545 2229 Fragments arranged by size: 2319 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 1108 Size: 1131 5059 PfoI T'CCnGG_A Cuts at: 3408 3408 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PmlI CAC'GTG Cuts at: 3247 3297 3247 Size: 50 6140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4259 4291 5772 5804 3404 Size: 32 823 32 1481 32 3790 Fragments arranged by size: 3790 1481 823 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 2109 2117 3425 2109 Size: 8 1308 4874 Fragments arranged by size: 4874 1308 8 PspXI vC'TCGA_Gb Cuts at: 3029 3029 Size: 6190 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3434 3466 3434 Size: 32 6158 PstI C_TGCA'G Cuts at: 830 2088 2220 2679 3345 3542 830 Size: 1258 132 459 666 197 3478 Fragments arranged by size: 3478 1258 666 459 197 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2707 3136 2707 Size: 429 5761 SacI G_AGCT'C Cuts at: 3417 3417 Size: 6190 SacII CC_GC'GG Cuts at: 2730 2730 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2084 2216 2675 3341 3538 4673 5354 Size: 219 1039 132 459 666 197 1135 681 Cuts at: 5354 5545 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1039 681 666 459 219 197 191 132 SfoI GGC'GCC Cuts at: 2640 3387 2640 Size: 747 5443 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 2108 2114 3430 3517 1872 Size: 236 6 1316 87 4545 Fragments arranged by size: 4545 1316 236 87 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3029 3416 4294 5195 5457 820 Size: 197 926 1086 387 878 901 262 1553 Fragments arranged by size: 1553 1086 926 901 878 387 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2096 3077 3288 3629 513 Size: 552 1031 981 211 341 3074 Fragments arranged by size: 3074 1031 981 552 341 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 4436 6148 Size: 274 80 33 51 1443 1245 1214 1712 Cuts at: 6148 96 Size: 138 Fragments arranged by size: 1712 1443 1245 1214 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 2889 2921 6150 6182 2583 Size: 32 274 32 3229 32 2591 Fragments arranged by size: 3229 2591 274 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 2377 2302 Size: 75 6115 XhoI C'TCGA_G Cuts at: 1943 3029 1943 Size: 1086 5104 XmaI C'CCGG_G Cuts at: 1870 2106 2112 3428 3515 1870 Size: 236 6 1316 87 4545 Fragments arranged by size: 4545 1316 236 87 6 XmnI GAAnn'nnTTC Cuts at: 4319 4319 Size: 6190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI RsrII SanDI SapI SexAI SfiI SrfI SwaI